Daniel Jacobsen: Difference between revisions
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==Notebook== | ==Notebook== | ||
[[Daniel:Notebook/HiResChrPaint|High Resolution Chromosome Painting]] | [[Daniel:Notebook/HiResChrPaint|High Resolution Chromosome Painting]] | ||
[[Daniel:Notebook/GenomeMiner|Genome Miner]] | |||
[[Daniel:Notebook/Haplotyping|Haplotyping]] | |||
[[Daniel:Notebook/Deconvolution|Methylation Deconvolution Project]] | |||
[[Daniel:Notebook/RNAFISH|RNA FISH Project]] | |||
[[Daniel:Notebook/RNAFACS|RNA FISH-FACS based sorting]] | |||
[[Daniel:Notebook/ComboLock|Combo-Lock]] | |||
[[Daniel:Notebook/PosSequencing|Positional Tissue Sequencing]] | |||
==Protocols== | ==Protocols== | ||
[[Daniel:Protocols/EtOHPrecip|Ethanol Precipitation]] | |||
{|class="wikitable" | |||
|-align="center" | |||
|width="120"|'''Purification Protocols''' | |||
|width="120"|'''CERC Protocols''' | |||
|width="120"|'''Gel Protocols''' | |||
|width="120"|'''FISH Protocols''' | |||
|width="120"|'''Misc Protocols''' | |||
|width="150"|'''Combo-Lock Protocols''' | |||
|-align="center" | |||
|[[Daniel:Protocols/Qiaquick_Column|Qiaquick Column Purification]] | |||
|[[Daniel:Protocols/FreezeCells|Freeze Cells (CERC)]] | |||
|[[Daniel:Protocols/TBUgel|Urea Gel Quantification]] | |||
|[[Daniel:Protocols/FISH|FISH (Fluorescence in situ Hybridization)]] | |||
|[[Daniel:Protocols/CPTSeq|CPT-Seq]] | |||
|[[Daniel:Protocols/AbOligoLink|Antibody-Oligo Linkage]] | |||
|-align="center" | |||
|[[Daniel:Protocols/ssDNA_Column|ssDNA Column (Zymo)]] | |||
|[[Daniel:Protocols/SplitCells|Splitting Cells]] | |||
| | |||
|[[Daniel:Protocols/AlexaDyeCouple|Alexa Fluorophore Dye Coupling]] | |||
|[[Daniel:Protocols/qPCR|qPCR]] | |||
|[[Daniel:Protocols/CProbeLigate|C Probe Hybridization]] | |||
|-align="center" | |||
|[[Daniel:Protocols/CentriSep|Centri-Sep Protocol (Princeton Separations)]] | |||
|[[Daniel:Protocols/HiMWDNAExtract|High MW DNA Extraction]] | |||
| | |||
|[[Daniel:Protocols/TissueFISH|FISH on Tissue Sections]] | |||
|[[Daniel:Protocols/NuclearAdhesion|Nuclear Adhesion to Slides]] | |||
|[[Daniel:Protocols/AbHybridization|Antibody Hybridization]] | |||
|-align="center" | |||
|[[Daniel:Protocols/EtOHPrecip|Ethanol Precipitation]] | |||
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|[[Daniel:Protocols/RNAScope|RNA Scope]] | |||
|[[Daniel:Protocols/SlidePAGE|PAGE Gel Creation on Slides]] | |||
|[[Daniel:Protocols/LatchPadlock|Latch and Padlock Hybridization]] | |||
|-align="center" | |||
|[[Daniel:Protocols/MagBeads|Magnetic Beads Purification]] | |||
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|[[Daniel:Protocols/RNASort|RNA FISH Cell Sorting]] | |||
|[[Daniel:Protocols/EmulsionPCR|Emulsion PCR]] | |||
|valign="center"|[[Daniel:Protocols/RCA|Circularization/Rolling Circle Amplification]] | |||
|-align="center" | |||
|[[Daniel:Protocols/AmiconUltra|Amicon Ultra Centrifugal Filters]] | |||
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|[[Daniel:Protocols/Stellaris|Stellaris Probes]] | |||
|[[Daniel:Protocols/Qubit|Qubit Quantification]] | |||
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|} | |||
==Cell Lines== | |||
{|class="wikitable" | |||
|-align="center" | |||
|width="120"|[http://www.atcc.org/products/all/CRL-5908.aspx#generalinformation NCI-h1975] | |||
|width="120"|[[Daniel:CellLines/U87MG|U-87MG Cell Line]] | |||
|width="120"|[[Daniel:CellLines/T98G|T98-G]] | |||
|width="120"|[[Daniel:CellLines/BE2C|BE2-C]] | |||
|width="120"| | |||
|-align="center" | |||
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|} | |||
==Reagents== | |||
[[Daniel:Reagents/DpnII|DpnII]] | |||
[[Daniel:Reagents/LambdaExonuclease|Lambda Exonuclease]] | |||
==Lab Meeting Presentations== | |||
[[Daniel:LabMeetings/HiResChrPaint|High Res Chromosome Painting]] | |||
==Freezer Boxes== | |||
[[Daniel:ProbeList|Stored Probes]] | |||
==Templates== | |||
[[Template:GelLanes|Gel Template]] | |||
[[Template:ProbeEntry|Probe Template]] | |||
==Training== | |||
Lab Safety/IIPP Training Certificate: [[File:Lab_saftey_jacobsen.pdf]] | |||
Stem Cell Ethics Training Certificate: [[File:Stem_Cell_Ethics_Jacobsen.pdf]] | |||
Annual Hazards Safety Training Certificate: [[File:Jacobsen_AnnualSafetyTraining2013.pdf]] | |||
Laboratory Safety Fundamentals Training Certificate: [[File:JacobsenLabSafetyFundamentals.pdf]] | |||
Bloodborne Pathogens Training Certificate: [[File:JacobsenDaniel_BBP_Training.pdf]] |
Latest revision as of 17:27, 13 May 2017
Notebook[edit]
High Resolution Chromosome Painting
Methylation Deconvolution Project
Protocols[edit]
Cell Lines[edit]
NCI-h1975 | U-87MG Cell Line | T98-G | BE2-C | |
Reagents[edit]
Lab Meeting Presentations[edit]
Freezer Boxes[edit]
Templates[edit]
Training[edit]
Lab Safety/IIPP Training Certificate: File:Lab saftey jacobsen.pdf Stem Cell Ethics Training Certificate: File:Stem Cell Ethics Jacobsen.pdf Annual Hazards Safety Training Certificate: File:Jacobsen AnnualSafetyTraining2013.pdf Laboratory Safety Fundamentals Training Certificate: File:JacobsenLabSafetyFundamentals.pdf Bloodborne Pathogens Training Certificate: File:JacobsenDaniel BBP Training.pdf