Daniel Jacobsen: Difference between revisions

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[[Daniel:Notebook/GenomeMiner|Genome Miner]]
[[Daniel:Notebook/GenomeMiner|Genome Miner]]
[[Daniel:Notebook/Haplotyping|Haplotyping]]
[[Daniel:Notebook/Deconvolution|Methylation Deconvolution Project]]
[[Daniel:Notebook/RNAFISH|RNA FISH Project]]
[[Daniel:Notebook/RNAFACS|RNA FISH-FACS based sorting]]
[[Daniel:Notebook/ComboLock|Combo-Lock]]
[[Daniel:Notebook/PosSequencing|Positional Tissue Sequencing]]


==Protocols==
==Protocols==
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{|class="wikitable"
{|class="wikitable"
|-align="center"
|-align="center"
|width="120"|'''Column Protocols'''
|width="120"|'''Purification Protocols'''
|width="120"|'''CERC Protocols'''
|width="120"|'''CERC Protocols'''
|width="120"|'''Gel Protocols'''
|width="120"|'''Gel Protocols'''
|width="120"|'''FISH Protocols'''
|width="120"|'''FISH Protocols'''
|width="120"|'''Misc Protocols'''
|width="120"|'''Misc Protocols'''
|width="150"|'''Combo-Lock Protocols'''


|-align="center"
|-align="center"
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|[[Daniel:Protocols/FreezeCells|Freeze Cells (CERC)]]
|[[Daniel:Protocols/FreezeCells|Freeze Cells (CERC)]]
|[[Daniel:Protocols/TBUgel|Urea Gel Quantification]]
|[[Daniel:Protocols/TBUgel|Urea Gel Quantification]]
|[[Daniel:Protocols/FISH|FISH (Fluorescence in situ Hybridization)]]
|[[Daniel:Protocols/CPTSeq|CPT-Seq]]
|[[Daniel:Protocols/AbOligoLink|Antibody-Oligo Linkage]]
|-align="center"
|[[Daniel:Protocols/ssDNA_Column|ssDNA Column (Zymo)]]
|[[Daniel:Protocols/SplitCells|Splitting Cells]]
|
|
|[[Daniel:Protocols/AlexaDyeCouple|Alexa Fluorophore Dye Coupling]]
|[[Daniel:Protocols/qPCR|qPCR]]
|[[Daniel:Protocols/CProbeLigate|C Probe Hybridization]]
|-align="center"
|[[Daniel:Protocols/CentriSep|Centri-Sep Protocol (Princeton Separations)]]
|[[Daniel:Protocols/HiMWDNAExtract|High MW DNA Extraction]]
|
|
|[[Daniel:Protocols/TissueFISH|FISH on Tissue Sections]]
|[[Daniel:Protocols/NuclearAdhesion|Nuclear Adhesion to Slides]]
|[[Daniel:Protocols/AbHybridization|Antibody Hybridization]]


|}
|-align="center"
|[[Daniel:Protocols/EtOHPrecip|Ethanol Precipitation]]
|
|
|[[Daniel:Protocols/RNAScope|RNA Scope]]
|[[Daniel:Protocols/SlidePAGE|PAGE Gel Creation on Slides]]
|[[Daniel:Protocols/LatchPadlock|Latch and Padlock Hybridization]]


|-align="center"
|[[Daniel:Protocols/MagBeads|Magnetic Beads Purification]]
|
|
|[[Daniel:Protocols/RNASort|RNA FISH Cell Sorting]]
|[[Daniel:Protocols/EmulsionPCR|Emulsion PCR]]
|valign="center"|[[Daniel:Protocols/RCA|Circularization/Rolling Circle Amplification]]




|-align="center"
|[[Daniel:Protocols/AmiconUltra|Amicon Ultra Centrifugal Filters]]
|
|
|[[Daniel:Protocols/Stellaris|Stellaris Probes]]
|[[Daniel:Protocols/Qubit|Qubit Quantification]]
|


|}


==Cell Lines==


{|class="wikitable"
|-align="center"
|width="120"|[http://www.atcc.org/products/all/CRL-5908.aspx#generalinformation NCI-h1975]
|width="120"|[[Daniel:CellLines/U87MG|U-87MG Cell Line]]
|width="120"|[[Daniel:CellLines/T98G|T98-G]]
|width="120"|[[Daniel:CellLines/BE2C|BE2-C]]
|width="120"|


{|class="wikitable"|
|-align="center"
|-align="center"
|width="120"|[[Daniel:Protocols/EtOHPrecip|Ethanol Precipitation]]
|
|width="120"|[[Daniel:Protocols/SplitCells|Splitting Cells]]
|
|width="120"|[[Daniel:Protocols/Qiaquick_Column|Qiaquick Column Purification]]
|
|width="120"|[[Daniel:Protocols/TBUgel|Urea Gel Quantification]]
|
|width="120"|[[Daniel:Protocols/AlexaDyeCouple|Alexa Fluorophore Dye Coupling]]
|
|width="120"|[[Daniel:Protocols/CentriSep|Centri-Sep Protocol (Princeton Separations)]]
 
|}
|}
[[Daniel:Protocols/FISH|FISH (Fluorescence in situ Hybridization)]]
[[Daniel:Protocols/ssDNA_Column|ssDNA Column (Zymo)]]
[[Daniel:Protocols/FreezeCells|Freeze Cells (CERC)]]


==Reagents==
==Reagents==

Latest revision as of 17:27, 13 May 2017

Notebook[edit]

High Resolution Chromosome Painting

Genome Miner

Haplotyping

Methylation Deconvolution Project

RNA FISH Project

RNA FISH-FACS based sorting

Combo-Lock

Positional Tissue Sequencing

Protocols[edit]

Purification Protocols CERC Protocols Gel Protocols FISH Protocols Misc Protocols Combo-Lock Protocols
Qiaquick Column Purification Freeze Cells (CERC) Urea Gel Quantification FISH (Fluorescence in situ Hybridization) CPT-Seq Antibody-Oligo Linkage
ssDNA Column (Zymo) Splitting Cells Alexa Fluorophore Dye Coupling qPCR C Probe Hybridization
Centri-Sep Protocol (Princeton Separations) High MW DNA Extraction FISH on Tissue Sections Nuclear Adhesion to Slides Antibody Hybridization
Ethanol Precipitation RNA Scope PAGE Gel Creation on Slides Latch and Padlock Hybridization
Magnetic Beads Purification RNA FISH Cell Sorting Emulsion PCR Circularization/Rolling Circle Amplification


Amicon Ultra Centrifugal Filters Stellaris Probes Qubit Quantification

Cell Lines[edit]

NCI-h1975 U-87MG Cell Line T98-G BE2-C

Reagents[edit]

DpnII

Lambda Exonuclease

Lab Meeting Presentations[edit]

High Res Chromosome Painting

Freezer Boxes[edit]

Stored Probes

Templates[edit]

Gel Template

Probe Template

Training[edit]

Lab Safety/IIPP Training Certificate: File:Lab saftey jacobsen.pdf
Stem Cell Ethics Training Certificate: File:Stem Cell Ethics Jacobsen.pdf
Annual Hazards Safety Training Certificate: File:Jacobsen AnnualSafetyTraining2013.pdf
Laboratory Safety Fundamentals Training Certificate:  File:JacobsenLabSafetyFundamentals.pdf
Bloodborne Pathogens Training Certificate: File:JacobsenDaniel BBP Training.pdf