Dan:Notebook/HiResChrPaint/2013-4-10: Difference between revisions
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Fit curve for ladder data | |||
[[Image:LambdaExo Digest GelQuant 041013.png|400px]] | |||
Final Results: | |||
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext> | |||
|- align="center" | |||
| width="65" height="12" | Sample | |||
| width="74" | ng/uL | |||
| width="65" | ng remaining | |||
|- align="center" | |||
| height="12" | V4S1 | |||
| align="right" | 5.19 | |||
| align="right" | 46.71 | |||
|- align="center" | |||
| height="12" | V4S2 | |||
| align="right" | 28.29 | |||
| align="right" | 254.61 | |||
|- align="center" | |||
| height="12" | V4S3 | |||
| align="right" | 27.33 | |||
| align="right" | 245.97 | |||
|- align="center" | |||
| height="12" | V4S4 | |||
| align="right" | 9.50 | |||
| align="right" | 85.5 | |||
|- align="center" | |||
| height="12" | V6S1 | |||
| Not Quantified | |||
| NA | |||
|- align="center" | |||
| height="12" | V6S2 | |||
| align="right" | 22.95 | |||
| align="right" | 206.55 | |||
|- align="center" | |||
| height="12" | V6S3 | |||
| align="right" | 24.12 | |||
| align="right" | 217.08 | |||
|- align="center" | |||
| height="12" | V6S4 | |||
| align="right" | 27.82 | |||
| align="right" | 250.38 | |||
|} | |} | ||
[[Daniel:Notebook/HiResChrPaint|Back to Calendar]] | [[Daniel:Notebook/HiResChrPaint|Back to Calendar]] |
Latest revision as of 19:21, 12 April 2013
LambdaExo Digestion Gel Quantification[edit]
Urea gel to quantify Lambda Exonuclease digestion from 4/5/2013
Urea Gel Protocol[edit]
Prerun gel for at least 20 minutes at 250V If the gel stops running before samples are ready just repeat Set up samples according to table below
Lane | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 |
Sample | 0.5L | 1L | 2L | V4S1 | V4S2 | V4S3 | V4S4 | V6S1 | V6S2 | V6S3 | V6S4 | 0.5L |
uL Sample | 0.5 | 1 | 2 | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 |
uL ddH20 | 5.5 | 5 | 4 | 5.5 | 5.5 | 5.5 | 5.5 | 5.5 | 5.5 | 5.5 | 5.5 | 5.5 |
uL 2x Urea Buffer | 6 | 6 | 6 | 6 | 6 | 6 | 6 | 6 | 6 | 6 | 6 | 6 |
Ran gel at 250V for 25 minutes
Gel Images[edit]
File:LambdaExo Digest 041013.jpg File:LambdaExo Digest Sampleid 041013.jpg
Quantification[edit]
Name | Sample | Volume | Minus Back | ng | sample ng | ng/uL |
INT*mm2 | ||||||
U1 | 100 bp 0.5 uL | 3734.900111 | 1179.300035 | 1.25 | ||
U2 | 200 bp 0.5 uL | 5710.70017 | 3216.350096 | 2.5 | ||
U3 | 100 bp 1 uL | 5355.74016 | 2704.900081 | 2.5 | ||
U4 | 200 bp 1 uL | 9250.050276 | 6494.800194 | 5 | ||
U5 | 100 bp 2 uL | 7312.240218 | 4959.430148 | 5 | ||
U6 | 200 bp 2 uL | 14064.53042 | 9710.270289 | 10 | ||
U7 | 100 bp 0.5 uL | 2633.210078 | 832.4200248 | 1.25 | ||
U8 | 200 bp 0.5 uL | 2938.250088 | 1623.040048 | 2.5 | ||
U9 | V4S1 | 8283.920247 | 2663.760079 | 2.60 | 5.19 | |
U10 | V4S2 | 29179.31087 | 15496.58046 | 14.15 | 28.29 | |
U11 | V4S3 | 26099.06078 | 14960.41045 | 13.66 | 27.33 | |
U12 | V4S4 | 10789.25032 | 5057.100151 | 4.75 | 9.50 | |
U13 | V6S2 | 18800.71056 | 12526.75037 | 11.47 | 22.95 | |
U14 | V6S3 | 21088.09063 | 13177.76039 | 12.06 | 24.12 | |
U15 | V6S4 | 22420.46067 | 15236.43045 | 13.91 | 27.82 | |
U16 | 2555.600076 | |||||
U17 | 2494.350074 | |||||
U18 | 2650.840079 | |||||
U19 | 2755.250082 | |||||
U20 | 2352.81007 | |||||
U21 | 4354.26013 | |||||
U22 | 1800.790054 | |||||
U23 | 1315.210039 | |||||
U24 | 5620.160167 | |||||
U25 | 13682.73041 | |||||
U26 | 11138.65033 | |||||
U27 | 5732.150171 | |||||
U28 | 6273.960187 | |||||
U29 | 7910.330236 | |||||
U30 | 7184.030214 |
Fit curve for ladder data
File:LambdaExo Digest GelQuant 041013.png
Final Results:
Sample | ng/uL | ng remaining |
V4S1 | 5.19 | 46.71 |
V4S2 | 28.29 | 254.61 |
V4S3 | 27.33 | 245.97 |
V4S4 | 9.50 | 85.5 |
V6S1 | Not Quantified | NA |
V6S2 | 22.95 | 206.55 |
V6S3 | 24.12 | 217.08 |
V6S4 | 27.82 | 250.38 |