Daniel:Protocols/Qiaquick Column: Difference between revisions
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>Djacobse (Created page with "=Qiaquick Column Purification= Qiagen: Catalog #28104 (50) or #28106 (250) ==Protocol== 1. Add 5X PB (binding buffer) to sample 2. Load sample onto column and spin for 1...") |
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=Qiaquick Column Purification= | =Qiaquick Column Purification= | ||
Qiagen: Catalog #28104 (50) or #28106 (250) | [[Daniel Jacobsen|Back to Main]] | ||
'''Qiagen: Catalog #28104 (50) or #28106 (250)''' | |||
==Protocol== | ==Protocol== | ||
<ol> | |||
<li>Add 5X PB (binding buffer) to sample</li> | |||
<li>Load sample onto column and spin for 1 minute at 14000 rpm; discard flow through</li> | |||
<li>Add 750 uL PE (wash buffer) to column and spin for 1 minute at 14000 rpm; discard flow through</li> | |||
<li>Dry spin column for 1 minute at 14000 rpm; discard flow through</li> | |||
<li>Let stand with cover open in fume hood for ~5 minutes</li> | |||
<li>Transfer column to a new 1.5 mL eppendorf tube</li> | |||
<li>Add ≥30 uL EB to column</li> | |||
<li>Let stand 1 minute</li> | |||
<li>Spin for 1 minute at 14000 rpm</li> | |||
</ol> |
Latest revision as of 16:40, 28 February 2017
Qiaquick Column Purification[edit]
Qiagen: Catalog #28104 (50) or #28106 (250)
Protocol[edit]
- Add 5X PB (binding buffer) to sample
- Load sample onto column and spin for 1 minute at 14000 rpm; discard flow through
- Add 750 uL PE (wash buffer) to column and spin for 1 minute at 14000 rpm; discard flow through
- Dry spin column for 1 minute at 14000 rpm; discard flow through
- Let stand with cover open in fume hood for ~5 minutes
- Transfer column to a new 1.5 mL eppendorf tube
- Add ≥30 uL EB to column
- Let stand 1 minute
- Spin for 1 minute at 14000 rpm