Daniel:Notebook/HiResChrPaint/2013-4-26: Difference between revisions

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  2. Add 200 uL nuclease free H20 to mixture (total 300 uL)
  2. Add 200 uL nuclease free H20 to mixture (total 300 uL)
  3. Add 900 uL 100% EtOH, 30 uL NaOAc, 2 uL glycoblue (2/300X)
  3. Add 900 uL 100% EtOH, 30 uL NaOAc, 2 uL glycoblue (2/300X)
  4. Incubate overnight at -80C
  4. Incubate overnight at -80C; Continued on [[Daniel:Notebook/HiResChrPaint/2013-4-27|4/27/2013]]

Latest revision as of 23:40, 27 April 2013

Probe Prep Test (Started 4/22/2013)[edit]

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Lambda Exonuclease Digest[edit]

1. Prepared samples according to following table
Sample ng/uL uL sample uL 10x buffer uL Lambda Exo uL ddH20 Total
V4S3A 102.2 30 5 2 13 50
V4S3B 86.8 30 5 2 13 50
V6S3A 80.1 30 5 2 13 50
V6S3B 77.9 30 5 2 13 50
2. Incubate for 1 hour at 37 C
3. Heat shock 75 C 10 minutes
4. Ethanol Precipitation

Ethanol Precipitation[edit]

1. Pooled samples A and B (total 100 uL)
2. Add 200 uL nuclease free H20 to mixture (total 300 uL)
3. Add 900 uL 100% EtOH, 30 uL NaOAc, 2 uL glycoblue (2/300X)
4. Incubate overnight at -80C; Continued on 4/27/2013