Matt:LabNotes/2013-5-16: Difference between revisions

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>Mzcai
(Created page with "Continued from: http://genome-tech.ucsd.edu/LabNotes/index.php/Matt:LabNotes/2013-5-15 ===Column Purification=== *Purified 6 tubes with 12 columns (100ul each) Nanodrop conc:")
 
>Mzcai
 
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===Column Purification===
===Column Purification===
*Purified 6 tubes with 12 columns (100ul each)
*Purified 6 tubes of 200ul enzyme digested probes with 12 columns (100ul in each column)
*Eluted 30ul each (180ul total per probe set)


Nanodrop conc:
Nanodrop ssDNA conc: <br>
0 gap: 58.78 ng/ul * 180ul = 10.58ug <br>
20 gap: 55.96 ng/ul * 180ul = 10.07ug <br>
 
Continued on: http://genome-tech.ucsd.edu/LabNotes/index.php/Matt:LabNotes/2013-5-17

Latest revision as of 20:03, 17 May 2013

Continued from: http://genome-tech.ucsd.edu/LabNotes/index.php/Matt:LabNotes/2013-5-15

Column Purification[edit]

  • Purified 6 tubes of 200ul enzyme digested probes with 12 columns (100ul in each column)
  • Eluted 30ul each (180ul total per probe set)

Nanodrop ssDNA conc:
0 gap: 58.78 ng/ul * 180ul = 10.58ug
20 gap: 55.96 ng/ul * 180ul = 10.07ug

Continued on: http://genome-tech.ucsd.edu/LabNotes/index.php/Matt:LabNotes/2013-5-17