Hosuk:LabNotes/2013-5-21: Difference between revisions
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====Confocal imaging with the samples at 05/20==== | ====Confocal imaging with the samples at [[Hosuk:LabNotes/2013-5-20|05/20]]==== | ||
*Cy3-probes that I currently use seemed to be excited higher at 488nm than 552nm, which is weird. | |||
*I've try lambda scanning to figure out where is emission spectrum range from the dye, | |||
*and the emission spectrum was around 570-590nm which is Cy3 range. | |||
*and the emission exited 488nm laser was stronger than 552nm. | |||
*Very anti intuition though, but I could see rolonies in cells. | |||
*And still there were too many big spots, which has to be removed! | |||
*Need to wash more. | |||
*I'll try normal protocol - from RT to RCA. | |||
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=====20x Oil, Position 3, Zoom = 1.00, resolution = 2048 x 2048===== | |||
*Raw images : [[Media:Experiment.lif_S4_50pmole_20x_p2au_z1.00_pos05_res2k_z0_ch00.tif|Ex:552nm]], [[Media:Experiment.lif_S4_50pmole_20x_p2au_z1.00_pos05_res2k_z0_ch02.tif|Ex:488nm]], [[Media:Experiment.lif_S4_50pmole_20x_p2au_z1.00_pos05_res2k_z0.tif|Merged]] | |||
[[File:S4_Confocal_20xOil_pos3_zoom1.00_res2k_2.png|800px]] | |||
=====20x Oil, Position 6, Zoom = 3.50, resolution = 512 x 512===== | =====20x Oil, Position 6, Zoom = 3.50, resolution = 512 x 512===== | ||
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[[File:S4_Confocal_20xOil_pos6_zoom3.50.png|800px]] | [[File:S4_Confocal_20xOil_pos6_zoom3.50.png|800px]] | ||
=====20x Oil, Position 6, Zoom = 3.50, resolution = 2048 x 2048===== | |||
*Raw images : [[Media:Experiment.lif_S4_50pmole_20x_p2au_z3.0_pos06_res2k_z0_ch00.tif|Ex:552nm]], [[Media:Experiment.lif_S4_50pmole_20x_p2au_z3.0_pos06_res2k_z0_ch02.tif|Ex:488nm]], [[Media:Experiment.lif_S4_50pmole_20x_p2au_z3.0_pos06_res2k_z0.tif|Merged]] | |||
[[File:S4_Confocal_20xOil_pos6_zoom3.50_res2k.png|800px]] |
Latest revision as of 02:49, 23 May 2013
Confocal imaging with the samples at 05/20[edit]
- Cy3-probes that I currently use seemed to be excited higher at 488nm than 552nm, which is weird.
- I've try lambda scanning to figure out where is emission spectrum range from the dye,
- and the emission spectrum was around 570-590nm which is Cy3 range.
- and the emission exited 488nm laser was stronger than 552nm.
- Very anti intuition though, but I could see rolonies in cells.
- And still there were too many big spots, which has to be removed!
- Need to wash more.
- I'll try normal protocol - from RT to RCA.
20x Oil, Position 3, Zoom = 1.00, resolution = 512 x 512[edit]
File:S4 Confocal 20xOil pos3 zoom1.00.png
20x Oil, Position 3, Zoom = 1.00, resolution = 2048 x 2048[edit]
File:S4 Confocal 20xOil pos3 zoom1.00 res2k 2.png
20x Oil, Position 6, Zoom = 3.50, resolution = 512 x 512[edit]
File:S4 Confocal 20xOil pos6 zoom3.50.png