Hosuk:LabNotes/2013-5-21: Difference between revisions

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====Confocal imaging with the samples at 05/20====
====Confocal imaging with the samples at [[Hosuk:LabNotes/2013-5-20|05/20]]====
*Cy3-probes that I currently use seemed to be excited higher at 488nm than 552nm, which is weird.
*I've try lambda scanning to figure out where is emission spectrum range from the dye,
*and the emission spectrum was around 570-590nm which is Cy3 range.
*and the emission exited 488nm laser was stronger than 552nm.
*Very anti intuition though, but I could see rolonies in cells.
*And still there were too many big spots, which has to be removed!
*Need to wash more.
 
 
*I'll try normal protocol - from RT to RCA.
 




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=====20x Oil, Position 3, Zoom = 1.00, resolution = 2048 x 2048=====
*Raw images : [[Media:Experiment.lif_S4_50pmole_20x_p2au_z1.00_pos05_res2k_z0_ch00.tif|Ex:552nm]], [[Media:Experiment.lif_S4_50pmole_20x_p2au_z1.00_pos05_res2k_z0_ch02.tif|Ex:488nm]], [[Media:Experiment.lif_S4_50pmole_20x_p2au_z1.00_pos05_res2k_z0.tif|Merged]]
[[File:S4_Confocal_20xOil_pos3_zoom1.00_res2k_2.png|800px]]


=====20x Oil, Position 6, Zoom = 3.50, resolution = 512 x 512=====
=====20x Oil, Position 6, Zoom = 3.50, resolution = 512 x 512=====
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[[File:S4_Confocal_20xOil_pos6_zoom3.50.png|800px]]
[[File:S4_Confocal_20xOil_pos6_zoom3.50.png|800px]]
=====20x Oil, Position 6, Zoom = 3.50, resolution = 2048 x 2048=====
*Raw images : [[Media:Experiment.lif_S4_50pmole_20x_p2au_z3.0_pos06_res2k_z0_ch00.tif|Ex:552nm]], [[Media:Experiment.lif_S4_50pmole_20x_p2au_z3.0_pos06_res2k_z0_ch02.tif|Ex:488nm]], [[Media:Experiment.lif_S4_50pmole_20x_p2au_z3.0_pos06_res2k_z0.tif|Merged]]
[[File:S4_Confocal_20xOil_pos6_zoom3.50_res2k.png|800px]]

Latest revision as of 02:49, 23 May 2013

Confocal imaging with the samples at 05/20[edit]

  • Cy3-probes that I currently use seemed to be excited higher at 488nm than 552nm, which is weird.
  • I've try lambda scanning to figure out where is emission spectrum range from the dye,
  • and the emission spectrum was around 570-590nm which is Cy3 range.
  • and the emission exited 488nm laser was stronger than 552nm.
  • Very anti intuition though, but I could see rolonies in cells.
  • And still there were too many big spots, which has to be removed!
  • Need to wash more.


  • I'll try normal protocol - from RT to RCA.



20x Oil, Position 3, Zoom = 1.00, resolution = 512 x 512[edit]

File:S4 Confocal 20xOil pos3 zoom1.00.png


20x Oil, Position 3, Zoom = 1.00, resolution = 2048 x 2048[edit]

File:S4 Confocal 20xOil pos3 zoom1.00 res2k 2.png

20x Oil, Position 6, Zoom = 3.50, resolution = 512 x 512[edit]

File:S4 Confocal 20xOil pos6 zoom3.50.png


20x Oil, Position 6, Zoom = 3.50, resolution = 2048 x 2048[edit]

File:S4 Confocal 20xOil pos6 zoom3.50 res2k.png