Daniel:Notebook/HiResChrPaint/2013-6-10: Difference between revisions

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==TBU Gel Quantification==
==TBU Gel Quantification==


[[Daniel:Protocols/TBUGel|Protocol 2]]
[[Daniel:Protocols/TBUgel|Protocol 2]]


  1. Sample matrix
  1. Sample matrix
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Volume Report: [[File:GelQuant_061013.xls]]
Volume Report: [[File:GelQuant_061013.xls]]


[[Image:GelQuant_061013.jpg|600px]]
{| class="wikitable"
|-
| [[Image:GelQuant_061013.jpg|400px]] || [[Image:GelQuant_051513_Volume.jpg|400px|Volumes used for quantification]]
|}
 
===Nanodrop Results===
 
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext>
|- style="font-weight:bold" align="center" valign="bottom"
| width="69" height="12" | Sample
| width="69" | ng/uL
| width="69" | ug in 10 uL
 
|-  align="center" valign="bottom"
|style="font-weight:bold" height="12" | V4S2
| align="center" | 119.9
| align="center" | 1.2
 
|-  align="center" valign="bottom"
|style="font-weight:bold" height="12" | V6S2
| align="center" | 122.9
| align="center" | 1.2


[[Image:GelQuant_051513_Volume.jpg]]
|-  align="center" valign="bottom"
|style="font-weight:bold" height="12" | V4S2*
| align="center" | 158.7
| align="center" | 1.6
 
|-  align="center" valign="bottom"
|style="font-weight:bold" height="12" | V6S2*
| align="center" | 153.4
| align="center" | 1.5
 
|}
Note: *denotes no aa-dUTP

Latest revision as of 22:53, 2 July 2013

Probe Prep 3 (Started 5/29/2013)[edit]

Back to Calendar

TBU Gel Quantification[edit]

Protocol 2

1. Sample matrix
Lane 2 3 4 5 6 7 8 9 10 11
Sample Low Mass Ladder Low Mass Ladder V4S2 V6S2 V4S2* V6S2* V4S2 V6S2 V4S2* V6S2*
uL sample 0.5 1 0.5 0.5 0.5 0.5 2** 2** 2** 2**
uL TBE 1.5 1 9.5 9.5 9.5 9.5 1 1 1 1
uL 2X Urea Buffer 2 2 10 10 10 10 1 1 1 1
Dilution NA NA 1:10 1:10 1:10 1:10 1:20 1:20 1:20 1:20
Loaded 4 4 4 4 4 4 4 4 4 4
2. Ran gel for 18 minutes at 250 V
3. Stained for 25 minutes with 3 uL SYBR Gold

Results[edit]

Volume Report: File:GelQuant 061013.xls

File:GelQuant 061013.jpg Volumes used for quantification

Nanodrop Results[edit]

Sample ng/uL ug in 10 uL
V4S2 119.9 1.2
V6S2 122.9 1.2
V4S2* 158.7 1.6
V6S2* 153.4 1.5

Note: *denotes no aa-dUTP