Blue:RNAseq Primer List: Difference between revisions
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>B1lake |
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==totoRNA primer sequence== | |||
* The 5' adaptors list | ==totoRNA 5' primer sequence== | ||
* The 5' TSO adaptors list | |||
{| {{table}} border=1 | {| {{table}} border=1 | ||
Line 19: | Line 20: | ||
| TSO.r04||iso_B_TSO_N10||iso-dCiso-dGiso-dCACACTCTTTCCCTACACGACGNNNUNNNNUNNNrGrGrG||/5Me-isodC//iisodG//iMe-isodC/ACACTCTTTCCCTACACGACGNNN/ideoxyU/NNNN/ideoxyU/NNN||\||rGrGrG | | TSO.r04||iso_B_TSO_N10||iso-dCiso-dGiso-dCACACTCTTTCCCTACACGACGNNNUNNNNUNNNrGrGrG||/5Me-isodC//iisodG//iMe-isodC/ACACTCTTTCCCTACACGACGNNN/ideoxyU/NNNN/ideoxyU/NNN||\||rGrGrG | ||
|- | |- | ||
| PB_PCR.F||PB_PCR.F||AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACG|||||| | | TSO.r05||IsoL_B_TSO_N8||T-iSp18-ACACTCTTTCCCTACACGACGNNNNUNNNNrGrG+G||T/iSp18/ACACTCTTTCCCTACACGACGNNNN/ideoxyU/NNNNrGrG+G|||\||rGrG+G | ||
|- | |||
| TSO.r04_N6||iso_B_TSO_N6||isodC-isodG-isodC-ACACTCTTTCCCTACACGACGNNNUNNNrGrGrG||/5Me-isodC//iisodG//iMe-isodC/ACACTCTTTCCCTACACGACGNNN/ideoxyU/NNNrGrGrG|||\||rGrGrG | |||
|- | |||
| TSO.r06||iso_SMARTer II A||isodC-isodG-isodC-AAGCAGTGGTATCAACGCAGAGTACrGrGrG||/5Me-isodC//iisodG//iMe-isodC/AAGCAGTGGTATCAACGCAGAGTACrGrGrG|||\|||rGrGrG | |||
|- | |||
| |||||||||| | |||
|- | |||
| PB_PCR.F||PB_PCR.F||AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACG||||\|| | |||
|- | |||
| PB_PCR.F*||PB_PCR.Fmod||AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACG||A*A*T*GATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACG||\|| | |||
|- | |||
| P1-STRT||P1-STRT||AATGATACGGCGACCACCGAGATCTAAGCAGTGGTATCAACGCAGAGT||||\|| | |||
|- | |||
| ILMN_PCR.F*||ILMN_PCR.Fmod||AATGATACGGCGACCACCGAGATC||A*A*T*GATACGGCGACCACCGAGATC||\|| | |||
|} | |} | ||
==totoRNA 3' primer sequence== | |||
* The 3' T20 adaptors list | |||
{| {{table}} border=1 | |||
| align="center" style="background:#f0f0f0;"|'''Tube ID''' | |||
| align="center" style="background:#f0f0f0;"|'''Primer full name''' | |||
| align="center" style="background:#f0f0f0;"|'''Primer full sequence''' | |||
| align="center" style="background:#f0f0f0;"|'''PE_R''' | |||
| align="center" style="background:#f0f0f0;"|'''Barcode''' | |||
| align="center" style="background:#f0f0f0;"|'''T20V''' | |||
|- | |||
| T20VN.r01||T20VN_PE_R||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTVN||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT||||TTTTTTTTTTTTTTTTTTTTVN | |||
|- | |||
| T20V.id01||T20V.BC8.PE_R.Indx1||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTCTGAGACTTTTTTTTTTTTTTTTTTTTV||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT||TCTGAGAC||TTTTTTTTTTTTTTTTTTTTV | |||
|- | |||
| T20V.id02||T20V.BC8.PE_R.Indx2||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTGCCCAAGTTTTTTTTTTTTTTTTTTTTV||||TGCCCAAG|| | |||
|- | |||
| T20V.id03||T20V.BC8.PE_R.Indx3||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGACTCGCTTTTTTTTTTTTTTTTTTTTTV||||GACTCGCT|| | |||
|- | |||
| T20V.id04||T20V.BC8.PE_R.Indx4||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCCACTCCATTTTTTTTTTTTTTTTTTTTV||||CCACTCCA|| | |||
|- | |||
| T20V.id05||T20V.BC8.PE_R.Indx5||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGAACAGGGTTTTTTTTTTTTTTTTTTTTV||||GAACAGGG|| | |||
|- | |||
| T20V.id06||T20V.BC8.PE_R.Indx6||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCTGCTGATTTTTTTTTTTTTTTTTTTTTV||||CTGCTGAT|| | |||
|- | |||
| T20V.id07||T20V.BC8.PE_R.Indx7||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCTAGTATCTTTTTTTTTTTTTTTTTTTTV||||CTAGTATC|| | |||
|- | |||
| T20V.id08||T20V.BC8.PE_R.Indx8||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGGCTGAAATTTTTTTTTTTTTTTTTTTTV||||GGCTGAAA|| | |||
|- | |||
| T20V.id09||T20V.BC8.PE_R.Indx9||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGAGTGCTTTTTTTTTTTTTTTTTTTTTTV||||GAGTGCTT|| | |||
|- | |||
| T20V.id10||T20V.BC8.PE_R.Indx10||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTATACGCCATTTTTTTTTTTTTTTTTTTTV||||ATACGCCA|| | |||
|- | |||
| T20V.id11||T20V.BC8.PE_R.Indx11||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTATGCGTTATTTTTTTTTTTTTTTTTTTTV||||ATGCGTTA|| | |||
|- | |||
| T20V.id12||T20V.BC8.PE_R.Indx12||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTCACCCTTTTTTTTTTTTTTTTTTTTTTV||||TCACCCTT|| | |||
|- | |||
| T20V.id13||T20V.BC8.PE_R.Indx13||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCCAACCGCTTTTTTTTTTTTTTTTTTTTV||||CCAACCGC|| | |||
|- | |||
| T20V.id14||T20V.BC8.PE_R.Indx14||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGGATTACCTTTTTTTTTTTTTTTTTTTTV||||GGATTACC|| | |||
|- | |||
| T20V.id15||T20V.BC8.PE_R.Indx15||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCAGAGACATTTTTTTTTTTTTTTTTTTTV||||CAGAGACA|| | |||
|- | |||
| T20V.id16||T20V.BC8.PE_R.Indx16||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCAATAGTGTTTTTTTTTTTTTTTTTTTTV||||CAATAGTG|| | |||
|- | |||
| T20V.id17||T20V.BC8.PE_R.Indx17||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGGTGGCCATTTTTTTTTTTTTTTTTTTTV||||GGTGGCCA|| | |||
|- | |||
| T20V.id18||T20V.BC8.PE_R.Indx18||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGGAGGGAGTTTTTTTTTTTTTTTTTTTTV||||GGAGGGAG|| | |||
|- | |||
| T20V.id19||T20V.BC8.PE_R.Indx19||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGTTGTCCTTTTTTTTTTTTTTTTTTTTTV||||GTTGTCCT|| | |||
|- | |||
| T20V.id20||T20V.BC8.PE_R.Indx20||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTTCGACCTTTTTTTTTTTTTTTTTTTTTV||||TTCGACC|| | |||
|- | |||
| T20V.id21||T20V.BC8.PE_R.Indx21||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGCTTATTGTTTTTTTTTTTTTTTTTTTTV||||GCTTATTG|| | |||
|- | |||
| T20V.id22||T20V.BC8.PE_R.Indx22||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCAACCTTTTTTTTTTTTTTTTTTTTTTTV||||CAACCTTT|| | |||
|- | |||
| T20V.id23||T20V.BC8.PE_R.Indx23||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCTTAGGTATTTTTTTTTTTTTTTTTTTTV||||CTTAGGTA|| | |||
|- | |||
| T20V.id24||T20V.BC8.PE_R.Indx24||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGCTCACCATTTTTTTTTTTTTTTTTTTTV||||GCTCACCA|| | |||
|- | |||
| T20V.id25||T20V.BC8.PE_R.Indx25||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTAGAAATGCTTTTTTTTTTTTTTTTTTTTV||||AGAAATGC|| | |||
|- | |||
| T20V.id26||T20V.BC8.PE_R.Indx26||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCTTACTGATTTTTTTTTTTTTTTTTTTTV||||TACTGATT|| | |||
|- | |||
| T20V.id27||T20V.BC8.PE_R.Indx27||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCTCACGGGTTTTTTTTTTTTTTTTTTTTV||||CTCACGGG|| | |||
|- | |||
| T20V.id28||T20V.BC8.PE_R.Indx28||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCACTGTCATTTTTTTTTTTTTTTTTTTTV|||||| | |||
|- | |||
| T20V.id29||T20V.BC8.PE_R.Indx29||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTTCTTGGGTTTTTTTTTTTTTTTTTTTTV|||||| | |||
|- | |||
| T20V.id30||T20V.BC8.PE_R.Indx30||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTACCGGCTTTTTTTTTTTTTTTTTTTTTTV|||||| | |||
|- | |||
| T20V.id31||T20V.BC8.PE_R.Indx31||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTAACCACATTTTTTTTTTTTTTTTTTTTV|||||| | |||
|- | |||
| T20V.id32||T20V.BC8.PE_R.Indx32||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTACGCGCGTTTTTTTTTTTTTTTTTTTTV|||||| | |||
|- | |||
| T20V.id33||T20V.BC8.PE_R.Indx33||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCCTCGATGTTTTTTTTTTTTTTTTTTTTV|||||| | |||
|- | |||
| T20V.id34||T20V.BC8.PE_R.Indx34||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTAGAGCTCATTTTTTTTTTTTTTTTTTTTV|||||| | |||
|- | |||
| T20V.id35||T20V.BC8.PE_R.Indx35||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTATGGTCCTTTTTTTTTTTTTTTTTTTTV|||||| | |||
|- | |||
| T20V.id36||T20V.BC8.PE_R.Indx36||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTGTCCGGCTTTTTTTTTTTTTTTTTTTTV|||||| | |||
|- | |||
| T20V.id37||T20V.BC8.PE_R.Indx37||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTAAGGAATCTTTTTTTTTTTTTTTTTTTTV|||||| | |||
|- | |||
| T20V.id38||T20V.BC8.PE_R.Indx38||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCATCGTAGTTTTTTTTTTTTTTTTTTTTV|||||| | |||
|- | |||
| T20V.id39||T20V.BC8.PE_R.Indx39||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGTGTTGAGTTTTTTTTTTTTTTTTTTTTV|||||| | |||
|- | |||
| T20V.id40||T20V.BC8.PE_R.Indx40||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTATGTATCCTTTTTTTTTTTTTTTTTTTTV|||||| | |||
|- | |||
| T20V.id41||T20V.BC8.PE_R.Indx41||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTGACAGGTTTTTTTTTTTTTTTTTTTTTV|||||| | |||
|- | |||
| T20V.id42||T20V.BC8.PE_R.Indx42||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTACGCCAATTTTTTTTTTTTTTTTTTTTV|||||| | |||
|- | |||
| T20V.id43||T20V.BC8.PE_R.Indx43||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGAAGGCAATTTTTTTTTTTTTTTTTTTTV|||||| | |||
|- | |||
| T20V.id44||T20V.BC8.PE_R.Indx44||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCACCCGAGTTTTTTTTTTTTTTTTTTTTV|||||| | |||
|- | |||
| T20V.id45||T20V.BC8.PE_R.Indx45||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGGTGTGTCTTTTTTTTTTTTTTTTTTTTV|||||| | |||
|- | |||
| T20V.id46||T20V.BC8.PE_R.Indx46||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTCTTGTGCTTTTTTTTTTTTTTTTTTTTV|||||| | |||
|- | |||
| T20V.id47||T20V.BC8.PE_R.Indx47||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTAGTACCTGTTTTTTTTTTTTTTTTTTTTV|||||| | |||
|- | |||
| T20V.id48||T20V.BC8.PE_R.Indx48||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTAGCGTCCTTTTTTTTTTTTTTTTTTTTTV|||||| | |||
|- | |||
| T20V.id49||T20V.BC8.PE_R.Indx49||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTGAACTGTTTTTTTTTTTTTTTTTTTTTV|||||| | |||
|- | |||
| T20V.id50||T20V.BC8.PE_R.Indx50||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCAATGCTCTTTTTTTTTTTTTTTTTTTTV|||||| | |||
|- | |||
| T20V.id51||T20V.BC8.PE_R.Indx51||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGTTAGGCGTTTTTTTTTTTTTTTTTTTTV|||||| | |||
|- | |||
| T20V.id52||T20V.BC8.PE_R.Indx52||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTAGACGCTTTTTTTTTTTTTTTTTTTTTV|||||| | |||
|- | |||
| T20V.id53||T20V.BC8.PE_R.Indx53||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCTGGTGTGTTTTTTTTTTTTTTTTTTTTV|||||| | |||
|- | |||
| T20V.id54||T20V.BC8.PE_R.Indx54||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTAGCGGTAATTTTTTTTTTTTTTTTTTTTV|||||| | |||
|- | |||
| T20V.id55||T20V.BC8.PE_R.Indx55||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCCTTAAGTTTTTTTTTTTTTTTTTTTTTV|||||| | |||
|- | |||
| T20V.id56||T20V.BC8.PE_R.Indx56||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGCGGTTTCTTTTTTTTTTTTTTTTTTTTV|||||| | |||
|- | |||
| T20V.id57||T20V.BC8.PE_R.Indx57||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTAAGCCTAGTTTTTTTTTTTTTTTTTTTTV|||||| | |||
|- | |||
| T20V.id58||T20V.BC8.PE_R.Indx58||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTAGCCAGGTTTTTTTTTTTTTTTTTTTTV|||||| | |||
|- | |||
| T20V.id59||T20V.BC8.PE_R.Indx59||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGTGGTAAATTTTTTTTTTTTTTTTTTTTV|||||| | |||
|- | |||
| T20V.id60||T20V.BC8.PE_R.Indx60||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCACATAGGTTTTTTTTTTTTTTTTTTTTV|||||| | |||
|- | |||
| T20V.id61||T20V.BC8.PE_R.Indx61||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGCCGGTGATTTTTTTTTTTTTTTTTTTTV|||||| | |||
|- | |||
| T20V.id62||T20V.BC8.PE_R.Indx62||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCCTAAACCTTTTTTTTTTTTTTTTTTTTV|||||| | |||
|- | |||
| T20V.id63||T20V.BC8.PE_R.Indx63||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCGTGTCCCTTTTTTTTTTTTTTTTTTTTV|||||| | |||
|- | |||
| T20V.id64||T20V.BC8.PE_R.Indx64||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTAGGGTAGGTTTTTTTTTTTTTTTTTTTTV|||||| | |||
|- | |||
| T20V.id65||T20V.BC8.PE_R.Indx65||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGGGAGATGTTTTTTTTTTTTTTTTTTTTV|||||| | |||
|- | |||
| T20V.id66||T20V.BC8.PE_R.Indx66||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTAGGAAGTATTTTTTTTTTTTTTTTTTTTV|||||| | |||
|- | |||
| T20V.id67||T20V.BC8.PE_R.Indx67||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGGGCATGCTTTTTTTTTTTTTTTTTTTTV|||||| | |||
|- | |||
| T20V.id68||T20V.BC8.PE_R.Indx68||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGTAGGTACTTTTTTTTTTTTTTTTTTTTV|||||| | |||
|- | |||
| T20V.id69||T20V.BC8.PE_R.Indx69||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGCTGGATTTTTTTTTTTTTTTTTTTTTTV|||||| | |||
|- | |||
| T20V.id70||T20V.BC8.PE_R.Indx70||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTGCTCCCTTTTTTTTTTTTTTTTTTTTTV|||||| | |||
|- | |||
| T20V.id71||T20V.BC8.PE_R.Indx71||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTAAGCGCCCTTTTTTTTTTTTTTTTTTTTV|||||| | |||
|- | |||
| T20V.id72||T20V.BC8.PE_R.Indx72||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCGACGGATTTTTTTTTTTTTTTTTTTTTV|||||| | |||
|- | |||
| T20V.id73||T20V.BC8.PE_R.Indx73||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGGATAGCGTTTTTTTTTTTTTTTTTTTTV|||||| | |||
|- | |||
| T20V.id74||T20V.BC8.PE_R.Indx74||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGCCCAGTGTTTTTTTTTTTTTTTTTTTTV|||||| | |||
|- | |||
| T20V.id75||T20V.BC8.PE_R.Indx75||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGTGAAGACTTTTTTTTTTTTTTTTTTTTV|||||| | |||
|- | |||
| T20V.id76||T20V.BC8.PE_R.Indx76||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTAATCAGGATTTTTTTTTTTTTTTTTTTTV|||||| | |||
|- | |||
| T20V.id77||T20V.BC8.PE_R.Indx77||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTATGCTTCTTTTTTTTTTTTTTTTTTTTTV|||||| | |||
|- | |||
| T20V.id78||T20V.BC8.PE_R.Indx78||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCCAAGCAGTTTTTTTTTTTTTTTTTTTTV|||||| | |||
|- | |||
| T20V.id79||T20V.BC8.PE_R.Indx79||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGAAGTCGTTTTTTTTTTTTTTTTTTTTTV|||||| | |||
|- | |||
| T20V.id80||T20V.BC8.PE_R.Indx80||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTCGCCGCATTTTTTTTTTTTTTTTTTTTV|||||| | |||
|- | |||
| T20V.id81||T20V.BC8.PE_R.Indx81||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTCAGTTACTTTTTTTTTTTTTTTTTTTTV|||||| | |||
|- | |||
| T20V.id82||T20V.BC8.PE_R.Indx82||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTAACGTGCTTTTTTTTTTTTTTTTTTTTV|||||| | |||
|- | |||
| T20V.id83||T20V.BC8.PE_R.Indx83||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTGTAGGATTTTTTTTTTTTTTTTTTTTTV|||||| | |||
|- | |||
| T20V.id84||T20V.BC8.PE_R.Indx84||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTAGCACATCTTTTTTTTTTTTTTTTTTTTV|||||| | |||
|- | |||
| T20V.id85||T20V.BC8.PE_R.Indx85||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGTGTCTTGTTTTTTTTTTTTTTTTTTTTV|||||| | |||
|- | |||
| T20V.id86||T20V.BC8.PE_R.Indx86||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTGCAACCATTTTTTTTTTTTTTTTTTTTV|||||| | |||
|- | |||
| T20V.id87||T20V.BC8.PE_R.Indx87||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCACTTGTCTTTTTTTTTTTTTTTTTTTTV|||||| | |||
|- | |||
| T20V.id88||T20V.BC8.PE_R.Indx88||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGAGTTTGGTTTTTTTTTTTTTTTTTTTTV|||||| | |||
|- | |||
| T20V.id89||T20V.BC8.PE_R.Indx89||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGTGCTGGATTTTTTTTTTTTTTTTTTTTV|||||| | |||
|- | |||
| T20V.id90||T20V.BC8.PE_R.Indx90||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGAGAGCGCTTTTTTTTTTTTTTTTTTTTV|||||| | |||
|- | |||
| T20V.id91||T20V.BC8.PE_R.Indx91||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCCGAACATTTTTTTTTTTTTTTTTTTTTV|||||| | |||
|- | |||
| T20V.id92||T20V.BC8.PE_R.Indx92||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTACTATGCGTTTTTTTTTTTTTTTTTTTTV|||||| | |||
|- | |||
| T20V.id93||T20V.BC8.PE_R.Indx93||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTTAGCACTTTTTTTTTTTTTTTTTTTTTV|||||| | |||
|- | |||
| T20V.id94||T20V.BC8.PE_R.Indx94||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTATGTCCATTTTTTTTTTTTTTTTTTTTTV|||||| | |||
|- | |||
| T20V.id95||T20V.BC8.PE_R.Indx95||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCCCTGCCTTTTTTTTTTTTTTTTTTTTTV|||||| | |||
|- | |||
| T20V.id96||T20V.BC8.PE_R.Indx96||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTTGTCAGCTTTTTTTTTTTTTTTTTTTTV|||||| | |||
|- | |||
| |||||||||| | |||
|- | |||
| N2.id1||PCR_R.N2Ind1||CAAGCAGAAGACGGCATACGAGATCGTGATCTCGGCATTCCTGCTGAACCGCTCTT||CAAGCAGAAGACGGCATACGAGAT||CGTGAT||CTCGGCATTCCTGCTGAACCGCTCTT | |||
|- | |||
| N2.id2||PCR_R.N2Ind2||CAAGCAGAAGACGGCATACGAGATACATCGCTCGGCATTCCTGCTGAACCGCTCTT||CAAGCAGAAGACGGCATACGAGAT||ACATCG||CTCGGCATTCCTGCTGAACCGCTCTT | |||
|- | |||
| N2.id3||PCR_R.N2Ind3||CAAGCAGAAGACGGCATACGAGATGCCTAACTCGGCATTCCTGCTGAACCGCTCTT||CAAGCAGAAGACGGCATACGAGAT||GCCTAA||CTCGGCATTCCTGCTGAACCGCTCTT | |||
|- | |||
| N2.id4||PCR_R.N2Ind4||CAAGCAGAAGACGGCATACGAGATTGGTCACTCGGCATTCCTGCTGAACCGCTCTT||CAAGCAGAAGACGGCATACGAGAT||TGGTCA||CTCGGCATTCCTGCTGAACCGCTCTT | |||
|- | |||
| N2.id5||PCR_R.N2Ind5||CAAGCAGAAGACGGCATACGAGATCACTGTCTCGGCATTCCTGCTGAACCGCTCTT||CAAGCAGAAGACGGCATACGAGAT||CACTGT||CTCGGCATTCCTGCTGAACCGCTCTT | |||
|- | |||
| N2.id6||PCR_R.N2Ind6||CAAGCAGAAGACGGCATACGAGATATTGGCCTCGGCATTCCTGCTGAACCGCTCT||CAAGCAGAAGACGGCATACGAGAT||ATTGGC||CTCGGCATTCCTGCTGAACCGCTCTT | |||
|- | |||
| N2.id7||PCR_R.N2Ind7||CAAGCAGAAGACGGCATACGAGATGATCTGCTCGGCATTCCTGCTGAACCGCTCTT||CAAGCAGAAGACGGCATACGAGAT||GATCTG||CTCGGCATTCCTGCTGAACCGCTCTT | |||
|- | |||
| N2.id8||PCR_R.N2Ind8||CAAGCAGAAGACGGCATACGAGATTCAAGTCTCGGCATTCCTGCTGAACCGCTCTT||CAAGCAGAAGACGGCATACGAGAT||TCAAGT||CTCGGCATTCCTGCTGAACCGCTCTT | |||
|- | |||
| N2.id9||PCR_R.N2Ind9||CAAGCAGAAGACGGCATACGAGATCTGATCCTCGGCATTCCTGCTGAACCGCTCTT||CAAGCAGAAGACGGCATACGAGAT||CTGATC||CTCGGCATTCCTGCTGAACCGCTCTT | |||
|- | |||
| N2.id10||PCR_R.N2Ind10||CAAGCAGAAGACGGCATACGAGATAAGCTACTCGGCATTCCTGCTGAACCGCTCTT||CAAGCAGAAGACGGCATACGAGAT||AAGCTA||CTCGGCATTCCTGCTGAACCGCTCTT | |||
|- | |||
| N2.id11||PCR_R.N2Ind11||CAAGCAGAAGACGGCATACGAGATGTAGCCCTCGGCATTCCTGCTGAACCGCTCTT||CAAGCAGAAGACGGCATACGAGAT||GTAGCC||CTCGGCATTCCTGCTGAACCGCTCTT | |||
|- | |||
| N2.id12||PCR_R.N2Ind12||CAAGCAGAAGACGGCATACGAGATTACAAGCTCGGCATTCCTGCTGAACCGCTCTT||CAAGCAGAAGACGGCATACGAGAT||TACAAG||CTCGGCATTCCTGCTGAACCGCTCTT | |||
|- | |||
| N2.id13||PCR_R.N2Ind13||||CAAGCAGAAGACGGCATACGAGAT||||CTCGGCATTCCTGCTGAACCGCTCTT | |||
|- | |||
| N2.id14||PCR_R.N2Ind14||||CAAGCAGAAGACGGCATACGAGAT||||CTCGGCATTCCTGCTGAACCGCTCTT | |||
|- | |||
| N2.id81||PCR_R.N2Ind81||CAAGCAGAAGACGGCATACGAGATCCAGGTCTCGGCATTCCTGCTGAACCGCTCTT||CAAGCAGAAGACGGCATACGAGAT||CCAGGT||CTCGGCATTCCTGCTGAACCGCTCTT | |||
|- | |||
| N2.id82||PCR_R.N2Ind82||CAAGCAGAAGACGGCATACGAGATCGTTTGCTCGGCATTCCTGCTGAACCGCTCTT||CAAGCAGAAGACGGCATACGAGAT||CGTTTG||CTCGGCATTCCTGCTGAACCGCTCTT | |||
|- | |||
| N2.id83||PCR_R.N2Ind83||CAAGCAGAAGACGGCATACGAGATGACCACCTCGGCATTCCTGCTGAACCGCTCTT||CAAGCAGAAGACGGCATACGAGAT||GACCAC||CTCGGCATTCCTGCTGAACCGCTCTT | |||
|- | |||
| N2.id84||PCR_R.N2Ind84||CAAGCAGAAGACGGCATACGAGATACAAGACTCGGCATTCCTGCTGAACCGCTCTT||CAAGCAGAAGACGGCATACGAGAT||ACAAGA||CTCGGCATTCCTGCTGAACCGCTCTT | |||
|- | |||
| N2.id85||PCR_R.N2Ind85||CAAGCAGAAGACGGCATACGAGATACCGCACTCGGCATTCCTGCTGAACCGCTCTT||CAAGCAGAAGACGGCATACGAGAT||ACCGCA||CTCGGCATTCCTGCTGAACCGCTCTT | |||
|- | |||
| N2.id86||PCR_R.N2Ind86||CAAGCAGAAGACGGCATACGAGATTGGGTACTCGGCATTCCTGCTGAACCGCTCTT||CAAGCAGAAGACGGCATACGAGAT||TGGGTA||CTCGGCATTCCTGCTGAACCGCTCTT | |||
|- | |||
| N2.id87||PCR_R.N2Ind87||CAAGCAGAAGACGGCATACGAGATATTCCGCTCGGCATTCCTGCTGAACCGCTCTT||CAAGCAGAAGACGGCATACGAGAT||ATTCCG||CTCGGCATTCCTGCTGAACCGCTCTT | |||
|- | |||
| N2.id88||PCR_R.N2Ind88||CAAGCAGAAGACGGCATACGAGATGAATGTCTCGGCATTCCTGCTGAACCGCTCTT||CAAGCAGAAGACGGCATACGAGAT||GAATGT||CTCGGCATTCCTGCTGAACCGCTCTT | |||
|- | |||
| N2.id89||PCR_R.N2Ind89||CAAGCAGAAGACGGCATACGAGATGCTGATCTCGGCATTCCTGCTGAACCGCTCTT||CAAGCAGAAGACGGCATACGAGAT||GCTGAT||CTCGGCATTCCTGCTGAACCGCTCTT | |||
|- | |||
| N2.id90||PCR_R.N2Ind90||CAAGCAGAAGACGGCATACGAGATAGTGCTCTCGGCATTCCTGCTGAACCGCTCTT||CAAGCAGAAGACGGCATACGAGAT||AGTGCT||CTCGGCATTCCTGCTGAACCGCTCTT | |||
|- | |||
| N2.id91||PCR_R.N2Ind91||CAAGCAGAAGACGGCATACGAGATCAGGGACTCGGCATTCCTGCTGAACCGCTCTT||CAAGCAGAAGACGGCATACGAGAT||CAGGGA||CTCGGCATTCCTGCTGAACCGCTCTT | |||
|- | |||
| N2.id92||PCR_R.N2Ind92||CAAGCAGAAGACGGCATACGAGATCATGCGCTCGGCATTCCTGCTGAACCGCTCTT||CAAGCAGAAGACGGCATACGAGAT||CATGCG||CTCGGCATTCCTGCTGAACCGCTCTT | |||
|- | |||
| N2.id93||PCR_R.N2Ind93||CAAGCAGAAGACGGCATACGAGATTGCCTACTCGGCATTCCTGCTGAACCGCTCTT||CAAGCAGAAGACGGCATACGAGAT||TGCCTA||CTCGGCATTCCTGCTGAACCGCTCTT | |||
|- | |||
| N2.id94||PCR_R.N2Ind94||CAAGCAGAAGACGGCATACGAGATCTATACCTCGGCATTCCTGCTGAACCGCTCTT||CAAGCAGAAGACGGCATACGAGAT||CTATAC||CTCGGCATTCCTGCTGAACCGCTCTT | |||
|- | |||
| N2.id95||PCR_R.N2Ind95||CAAGCAGAAGACGGCATACGAGATCCGAGTCTCGGCATTCCTGCTGAACCGCTCTT||CAAGCAGAAGACGGCATACGAGAT||CCGAGT||CTCGGCATTCCTGCTGAACCGCTCTT | |||
|- | |||
| N2.id96||PCR_R.N2Ind96||CAAGCAGAAGACGGCATACGAGATACCTGCCTCGGCATTCCTGCTGAACCGCTCTT||CAAGCAGAAGACGGCATACGAGAT||ACCTGC||CTCGGCATTCCTGCTGAACCGCTCTT | |||
|- | |||
| |||||||||| | |||
|- | |||
| PGR_PCR_R||||CAAGCAGAAGACGGCATACGAGATCTCGGCATTCCTGCTGAACCGCTCTT||||CGAGAT|| | |||
|- | |||
| PGR_PCR_Rb||PGR_PCR_Rmod||CAAGCAGAAGACGGCATACGAGATCTCGGCATTCCTGCTGAACCGCTCTT||/5Phos/CAAGCAGAAGACGGCATACGAGATCTCGGCATTCCTGCTGAACCGCTCTT|||| | |||
|- | |||
| ILMN_PCR_Rb||ILMN_PCR_Rmod||CAAGCAGAAGACGGCATACGAGAT||/5Phos/CAAGCAGAAGACGGCATACGAGAT|||| | |||
|- | |||
|} | |||
==IVT 5' primer sequence== | |||
* The 5' adaptors list | |||
{| {{table}} border=1 | |||
| align="center" style="background:#f0f0f0;"|'''Primer full name''' | |||
| align="center" style="background:#f0f0f0;"|'''Primer full sequence''' | |||
| align="center" style="background:#f0f0f0;"|'''PB_PCR_F Complementary Seq''' | |||
| align="center" style="background:#f0f0f0;"|'''Random Sequence''' | |||
|- | |||
| RL.IVT.N6||ACACTCTTTCCCTACACGACGNNNNNN||ACACTCTTTCCCTACACGACG||NNNNNN | |||
|- | |||
| RL.IVT.N9||ACACTCTTTCCCTACACGACGNNNNNNNNN||ACACTCTTTCCCTACACGACG||NNNNNNNNN | |||
|- | |||
|} | |||
==IVT 3' primer sequence== | |||
* The 3' adaptors list | |||
{| {{table}} border=1 | |||
| align="center" style="background:#f0f0f0;"|'''Primer full name''' | |||
| align="center" style="background:#f0f0f0;"|'''Primer full sequence''' | |||
| align="center" style="background:#f0f0f0;"|'''T20 Complementary Seq''' | |||
| align="center" style="background:#f0f0f0;"|'''Novel T7 Seq''' | |||
|- | |||
| RL.Add.T7||CTTTAATTATGCTGAGTGATATCCCCGTAAGGACGACTTGGCGAGAA||GCATTCCTGCTGAACCGCTCTT||CTTTAATTATGCTGAGTGATATCCC | |||
|- | |||
|} | |||
== MARS-Seq/STRT-Seq/Smart-Seq2/Quartz-Seq Primer Sequences == | |||
{| {{table}} border=1 | |||
| align="center" style="background:#f0f0f0;"|'''Protocol''' | |||
| align="center" style="background:#f0f0f0;"|'''Primer full name''' | |||
| align="center" style="background:#f0f0f0;"|'''Primer full sequence''' | |||
| align="center" style="background:#f0f0f0;"|'''Modification''' | |||
| align="center" style="background:#f0f0f0;"|'''Barcode Seq''' | |||
|- | |||
| MARS-Seq||MARS_T20N_id01||CGATTGAGGCCGGTAATACGACTCACTATAGGGGCGACGTGTGCTCTTCCGATCTCGTGATNNNNTTTTTTTTTTTTTTTTTTTTN||N/A||CGTGAT | |||
|- | |||
| MARS-Seq||MARS_T20N_id02||CGATTGAGGCCGGTAATACGACTCACTATAGGGGCGACGTGTGCTCTTCCGATCTACATCGNNNNTTTTTTTTTTTTTTTTTTTTN||N/A||ACATCG | |||
|- | |||
| MARS-Seq||MARS_T20N_id03||CGATTGAGGCCGGTAATACGACTCACTATAGGGGCGACGTGTGCTCTTCCGATCTGCCTAANNNNTTTTTTTTTTTTTTTTTTTTN||N/A||GCCTAA | |||
|- | |||
| MARS-Seq||MARS_T20N_id04||CGATTGAGGCCGGTAATACGACTCACTATAGGGGCGACGTGTGCTCTTCCGATCTTGGTCANNNNTTTTTTTTTTTTTTTTTTTTN||N/A||TGGTCA | |||
|- | |||
| MARS-Seq||MARS_T20N_id05||CGATTGAGGCCGGTAATACGACTCACTATAGGGGCGACGTGTGCTCTTCCGATCTCACTGTNNNNTTTTTTTTTTTTTTTTTTTTN||N/A||CACTGT | |||
|- | |||
| MARS-Seq||MARS_T20N_id06||CGATTGAGGCCGGTAATACGACTCACTATAGGGGCGACGTGTGCTCTTCCGATCTATTGGCNNNNTTTTTTTTTTTTTTTTTTTTN||N/A||ATTGGC | |||
|- | |||
| MARS-Seq||MARS_T20N_id07||CGATTGAGGCCGGTAATACGACTCACTATAGGGGCGACGTGTGCTCTTCCGATCTGATCTGNNNNTTTTTTTTTTTTTTTTTTTTN||N/A||GATCTG | |||
|- | |||
| MARS-Seq||MARS_T20N_id08||CGATTGAGGCCGGTAATACGACTCACTATAGGGGCGACGTGTGCTCTTCCGATCTTCAAGTNNNNTTTTTTTTTTTTTTTTTTTTN||N/A||TCAAGT | |||
|- | |||
| MARS-Seq||MARS_T20N_id09||CGATTGAGGCCGGTAATACGACTCACTATAGGGGCGACGTGTGCTCTTCCGATCTCTGATCNNNNTTTTTTTTTTTTTTTTTTTTN||N/A||CTGATC | |||
|- | |||
| MARS-Seq||MARS_T20N_id10||CGATTGAGGCCGGTAATACGACTCACTATAGGGGCGACGTGTGCTCTTCCGATCTAAGCTANNNNTTTTTTTTTTTTTTTTTTTTN||N/A||AAGCTA | |||
|- | |||
| MARS-Seq||MARS_RT_2||TCTAGCCTTCTCGCAGCACATC||N/A|| | |||
|- | |||
| MARS-Seq||P5_Rd1_PCR_F||AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT||N/A|| | |||
|- | |||
| MARS-Seq||P7_Rd2_PCR_R||CAAGCAGAAGACGGCATACGAGATGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT||N/A|| | |||
|- | |||
| MARS-Seq||MARS_lig_NNNX4_ix1||/5Phos/GACTNNNAGATCGGAAGAGCGTCGTGTAG/3SpC3/||5Phos/3SpC3||GACT | |||
|- | |||
| MARS-Seq||MARS_lig_NNNX4_ix2||/5Phos/CATGNNNAGATCGGAAGAGCGTCGTGTAG/3SpC3/||5Phos/3SpC3||CATG | |||
|- | |||
| MARS-Seq||MARS_lig_NNNX4_ix3||/5Phos/CCAANNNAGATCGGAAGAGCGTCGTGTAG/3SpC3/||5Phos/3SpC3||CCAA | |||
|- | |||
| MARS-Seq||MARS_lig_NNNX4_ix4||/5Phos/CTGTNNNAGATCGGAAGAGCGTCGTGTAG/3SpC3/||5Phos/3SpC3||CTGT | |||
|- | |||
| MARS-Seq||MARS_lig_NNNX4_ix5||/5Phos/GTAGNNNAGATCGGAAGAGCGTCGTGTAG/3SpC3/||5Phos/3SpC3||GTAG | |||
|- | |||
| MARS-Seq||MARS_lig_NNNX4_ix6||/5Phos/TGATNNNAGATCGGAAGAGCGTCGTGTAG/3SpC3/||5Phos/3SpC3||TGAT | |||
|- | |||
| Smart-seq2||Smart2-T30VN||AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTVN||N/A|| | |||
|- | |||
| Smart-seq2||Smart2_ISPCR||AAGCAGTGGTATCAACGCAGAGT||N/A|| | |||
|- | |||
| Smart-Seq2||Smart2-TSO||AAGCAGTGGTATCAACGCAGAGTACATrGrG+G||rGrG+G (LNA)|| | |||
|- | |||
| UMI-STRT-Seq||C1-P1-RNA-TSO||Bio-AAUGAUACGGCGACCACCGAUNNNNNGGG||RNA|| | |||
|- | |||
| UMI-STRT-Seq||C1-P1-T31||Bio-AATGATACGGCGACCACCGATCGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT|||| | |||
|- | |||
| UMI-STRT-Seq||C1-P1-PCR-2||Bio-GAATGATACGGCGACCACCGAT|||| | |||
|- | |||
| UMI-STRT-Seq||C1-TN5-U||PHO-CTGTCTCTTATACACATCTGACGC|||| | |||
|- | |||
| Quartz-Seq||RT primer (WTA)||TATAGAATTCGCGGCCGCTCGCGATAATACGACTCACTATAGGGCGTTTTTTTTTTTTTTTTTTTTTTTT||N/A|| | |||
|- | |||
| Quartz-Seq||Tagging primer||TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTT||N/A|| | |||
|- | |||
| Quartz-Seq||Suppression primer||(NH2)-GTATAGAATTCGCGGCCGCTCGCGAT|||| | |||
|- | |||
|} | |||
==NexteraXT Primers== | |||
{| {{table}} border=1 | |||
| align="center" style="background:#f0f0f0;"|'''Primer''' | |||
| align="center" style="background:#f0f0f0;"|'''Sequence''' | |||
| align="center" style="background:#f0f0f0;"|'''Index Sequence''' | |||
|- | |||
| NexteraXT.N7id01||CAAGCAGAAGACGGCATACGAGATCGTGATGTCTCGTGGGCTCGG||CGTGAT | |||
|- | |||
| NexteraXT.N7id02||CAAGCAGAAGACGGCATACGAGATACATCGGTCTCGTGGGCTCGG||ACATCG | |||
|- | |||
| NexteraXT.N7id03||CAAGCAGAAGACGGCATACGAGATCGACTCGTCTCGTGGGCTCGG||CGACTC | |||
|- | |||
| NexteraXT.N7id04||CAAGCAGAAGACGGCATACGAGATTGGTCAGTCTCGTGGGCTCGG||TGGTCA | |||
|- | |||
| NexteraXT.N7id05||CAAGCAGAAGACGGCATACGAGATCACTGTGTCTCGTGGGCTCGG||CACTGT | |||
|- | |||
| NexteraXT.N7id06||CAAGCAGAAGACGGCATACGAGATATTGGCGTCTCGTGGGCTCGG||ATTGGC | |||
|- | |||
| NexteraXT.N7id07||CAAGCAGAAGACGGCATACGAGATGATCTGGTCTCGTGGGCTCGG||GATCTG | |||
|- | |||
| NexteraXT.N7id08||CAAGCAGAAGACGGCATACGAGATTCAAGTGTCTCGTGGGCTCGG||TCAAGT | |||
|- | |||
| NexteraXT.N7id09||CAAGCAGAAGACGGCATACGAGATGAGCTCGTCTCGTGGGCTCGG||GAGCTC | |||
|- | |||
| NexteraXT.N7id10||CAAGCAGAAGACGGCATACGAGATAAGCTAGTCTCGTGGGCTCGG||AAGCTA | |||
|- | |||
| NexteraXT.N7id11||CAAGCAGAAGACGGCATACGAGATGTAGCCGTCTCGTGGGCTCGG||GTAGCC | |||
|- | |||
| NexteraXT.N7id12||CAAGCAGAAGACGGCATACGAGATTACAAGGTCTCGTGGGCTCGG||TACAAG | |||
|- | |||
| NexteraXT.N7id13||CAAGCAGAAGACGGCATACGAGATTCATGGGTCTCGTGGGCTCGG||TCATGG | |||
|- | |||
| NexteraXT.N7id14||CAAGCAGAAGACGGCATACGAGATTGTCTTGTCTCGTGGGCTCGG||TGTCTT | |||
|- | |||
| NexteraXT.N7id15||CAAGCAGAAGACGGCATACGAGATAGGAAGGTCTCGTGGGCTCGG||AGGAAG | |||
|- | |||
| NexteraXT.N7id16||CAAGCAGAAGACGGCATACGAGATAACCCCGTCTCGTGGGCTCGG||AACCCC | |||
|- | |||
| NexteraXT.N7id17||CAAGCAGAAGACGGCATACGAGATGATGAGGTCTCGTGGGCTCGG||GATGAG | |||
|- | |||
| NexteraXT.N7id18||CAAGCAGAAGACGGCATACGAGATTGAACTGTCTCGTGGGCTCGG||TGAACT | |||
|- | |||
| NexteraXT.N7id19||CAAGCAGAAGACGGCATACGAGATTGCGTCGTCTCGTGGGCTCGG||TGCGTC | |||
|- | |||
| NexteraXT.N7id20||CAAGCAGAAGACGGCATACGAGATGACAGGGTCTCGTGGGCTCGG||GACAGG | |||
|- | |||
| NexteraXT.N7id21||CAAGCAGAAGACGGCATACGAGATGGGTTGGTCTCGTGGGCTCGG||GGGTTG | |||
|- | |||
| NexteraXT.N7id22||CAAGCAGAAGACGGCATACGAGATTCCGAGGTCTCGTGGGCTCGG||TCCGAG | |||
|- | |||
| NexteraXT.N7id23||CAAGCAGAAGACGGCATACGAGATTTTCGAGTCTCGTGGGCTCGG||TTTCGA | |||
|- | |||
| NexteraXT.N7id24||CAAGCAGAAGACGGCATACGAGATGCGAATGTCTCGTGGGCTCGG||GCGAAT | |||
|- | |||
| NexteraXT.S501||AATGATACGGCGACCACCGAGATCTACACTAGATCGCTCGTCGGCAGCGTC||TAGATCGC | |||
|- | |||
|} | |||
==Sequencing Primers== | |||
{| {{table}} border=1 | |||
| align="center" style="background:#f0f0f0;"|'''Primer full name''' | |||
| align="center" style="background:#f0f0f0;"|'''Primer full sequence''' | |||
| align="center" style="background:#f0f0f0;"|'''Protocol''' | |||
| align="center" style="background:#f0f0f0;"|'''Read''' | |||
|- | |||
| totoRNAseq Read 1 v2 || CCACCGAGATCTACACTCTTTCCCTACACGACG || totoRNAseq || Read1 | |||
|- | |||
| N2RevSeq2 || CTCGGCATTCCTGCTGAACCGCTCTTCCGATCT || totoRNAseq || Read2 | |||
|- | |||
| N2IndSeq || AAGAGCGGTTCAGCAGGAATGCCGAG || totoRNAseq || N2 index Read | |||
|- | |||
| STRT-SEQ v3 || GATCTAAGCAGTGGTATCAACGCAGAGTACGGG || totoRNAseq || Read1 | |||
|- | |||
|} | |||
==Zhang Lab Indexes== | |||
[https://docs.google.com/spreadsheets/d/1F2L2NpqYCXYwYPk1WQdJDkj3yZKMJftYfeYUHtiXCL4/pubhtml# Index List] |
Latest revision as of 00:48, 30 July 2014
Adaptor Structure[edit]
totoRNA 5' primer sequence[edit]
- The 5' TSO adaptors list
Tube ID | Primer full name | Primer full sequence | PE_F-N10 | Barcode | TSO |
TSO.r04 | iso_B_TSO_N10 | iso-dCiso-dGiso-dCACACTCTTTCCCTACACGACGNNNUNNNNUNNNrGrGrG | /5Me-isodC//iisodG//iMe-isodC/ACACTCTTTCCCTACACGACGNNN/ideoxyU/NNNN/ideoxyU/NNN | \ | rGrGrG |
TSO.r05 | IsoL_B_TSO_N8 | T-iSp18-ACACTCTTTCCCTACACGACGNNNNUNNNNrGrG+G | T/iSp18/ACACTCTTTCCCTACACGACGNNNN/ideoxyU/NNNNrGrG+G | \ | rGrG+G |
TSO.r04_N6 | iso_B_TSO_N6 | isodC-isodG-isodC-ACACTCTTTCCCTACACGACGNNNUNNNrGrGrG | /5Me-isodC//iisodG//iMe-isodC/ACACTCTTTCCCTACACGACGNNN/ideoxyU/NNNrGrGrG | \ | rGrGrG |
TSO.r06 | iso_SMARTer II A | isodC-isodG-isodC-AAGCAGTGGTATCAACGCAGAGTACrGrGrG | /5Me-isodC//iisodG//iMe-isodC/AAGCAGTGGTATCAACGCAGAGTACrGrGrG | \ | rGrGrG |
PB_PCR.F | PB_PCR.F | AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACG | \ | ||
PB_PCR.F* | PB_PCR.Fmod | AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACG | A*A*T*GATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACG | \ | |
P1-STRT | P1-STRT | AATGATACGGCGACCACCGAGATCTAAGCAGTGGTATCAACGCAGAGT | \ | ||
ILMN_PCR.F* | ILMN_PCR.Fmod | AATGATACGGCGACCACCGAGATC | A*A*T*GATACGGCGACCACCGAGATC | \ |
totoRNA 3' primer sequence[edit]
- The 3' T20 adaptors list
Tube ID | Primer full name | Primer full sequence | PE_R | Barcode | T20V |
T20VN.r01 | T20VN_PE_R | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTVN | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT | TTTTTTTTTTTTTTTTTTTTVN | |
T20V.id01 | T20V.BC8.PE_R.Indx1 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTCTGAGACTTTTTTTTTTTTTTTTTTTTV | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT | TCTGAGAC | TTTTTTTTTTTTTTTTTTTTV |
T20V.id02 | T20V.BC8.PE_R.Indx2 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTGCCCAAGTTTTTTTTTTTTTTTTTTTTV | TGCCCAAG | ||
T20V.id03 | T20V.BC8.PE_R.Indx3 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGACTCGCTTTTTTTTTTTTTTTTTTTTTV | GACTCGCT | ||
T20V.id04 | T20V.BC8.PE_R.Indx4 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCCACTCCATTTTTTTTTTTTTTTTTTTTV | CCACTCCA | ||
T20V.id05 | T20V.BC8.PE_R.Indx5 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGAACAGGGTTTTTTTTTTTTTTTTTTTTV | GAACAGGG | ||
T20V.id06 | T20V.BC8.PE_R.Indx6 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCTGCTGATTTTTTTTTTTTTTTTTTTTTV | CTGCTGAT | ||
T20V.id07 | T20V.BC8.PE_R.Indx7 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCTAGTATCTTTTTTTTTTTTTTTTTTTTV | CTAGTATC | ||
T20V.id08 | T20V.BC8.PE_R.Indx8 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGGCTGAAATTTTTTTTTTTTTTTTTTTTV | GGCTGAAA | ||
T20V.id09 | T20V.BC8.PE_R.Indx9 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGAGTGCTTTTTTTTTTTTTTTTTTTTTTV | GAGTGCTT | ||
T20V.id10 | T20V.BC8.PE_R.Indx10 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTATACGCCATTTTTTTTTTTTTTTTTTTTV | ATACGCCA | ||
T20V.id11 | T20V.BC8.PE_R.Indx11 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTATGCGTTATTTTTTTTTTTTTTTTTTTTV | ATGCGTTA | ||
T20V.id12 | T20V.BC8.PE_R.Indx12 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTCACCCTTTTTTTTTTTTTTTTTTTTTTV | TCACCCTT | ||
T20V.id13 | T20V.BC8.PE_R.Indx13 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCCAACCGCTTTTTTTTTTTTTTTTTTTTV | CCAACCGC | ||
T20V.id14 | T20V.BC8.PE_R.Indx14 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGGATTACCTTTTTTTTTTTTTTTTTTTTV | GGATTACC | ||
T20V.id15 | T20V.BC8.PE_R.Indx15 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCAGAGACATTTTTTTTTTTTTTTTTTTTV | CAGAGACA | ||
T20V.id16 | T20V.BC8.PE_R.Indx16 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCAATAGTGTTTTTTTTTTTTTTTTTTTTV | CAATAGTG | ||
T20V.id17 | T20V.BC8.PE_R.Indx17 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGGTGGCCATTTTTTTTTTTTTTTTTTTTV | GGTGGCCA | ||
T20V.id18 | T20V.BC8.PE_R.Indx18 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGGAGGGAGTTTTTTTTTTTTTTTTTTTTV | GGAGGGAG | ||
T20V.id19 | T20V.BC8.PE_R.Indx19 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGTTGTCCTTTTTTTTTTTTTTTTTTTTTV | GTTGTCCT | ||
T20V.id20 | T20V.BC8.PE_R.Indx20 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTTCGACCTTTTTTTTTTTTTTTTTTTTTV | TTCGACC | ||
T20V.id21 | T20V.BC8.PE_R.Indx21 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGCTTATTGTTTTTTTTTTTTTTTTTTTTV | GCTTATTG | ||
T20V.id22 | T20V.BC8.PE_R.Indx22 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCAACCTTTTTTTTTTTTTTTTTTTTTTTV | CAACCTTT | ||
T20V.id23 | T20V.BC8.PE_R.Indx23 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCTTAGGTATTTTTTTTTTTTTTTTTTTTV | CTTAGGTA | ||
T20V.id24 | T20V.BC8.PE_R.Indx24 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGCTCACCATTTTTTTTTTTTTTTTTTTTV | GCTCACCA | ||
T20V.id25 | T20V.BC8.PE_R.Indx25 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTAGAAATGCTTTTTTTTTTTTTTTTTTTTV | AGAAATGC | ||
T20V.id26 | T20V.BC8.PE_R.Indx26 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCTTACTGATTTTTTTTTTTTTTTTTTTTV | TACTGATT | ||
T20V.id27 | T20V.BC8.PE_R.Indx27 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCTCACGGGTTTTTTTTTTTTTTTTTTTTV | CTCACGGG | ||
T20V.id28 | T20V.BC8.PE_R.Indx28 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCACTGTCATTTTTTTTTTTTTTTTTTTTV | |||
T20V.id29 | T20V.BC8.PE_R.Indx29 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTTCTTGGGTTTTTTTTTTTTTTTTTTTTV | |||
T20V.id30 | T20V.BC8.PE_R.Indx30 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTACCGGCTTTTTTTTTTTTTTTTTTTTTTV | |||
T20V.id31 | T20V.BC8.PE_R.Indx31 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTAACCACATTTTTTTTTTTTTTTTTTTTV | |||
T20V.id32 | T20V.BC8.PE_R.Indx32 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTACGCGCGTTTTTTTTTTTTTTTTTTTTV | |||
T20V.id33 | T20V.BC8.PE_R.Indx33 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCCTCGATGTTTTTTTTTTTTTTTTTTTTV | |||
T20V.id34 | T20V.BC8.PE_R.Indx34 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTAGAGCTCATTTTTTTTTTTTTTTTTTTTV | |||
T20V.id35 | T20V.BC8.PE_R.Indx35 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTATGGTCCTTTTTTTTTTTTTTTTTTTTV | |||
T20V.id36 | T20V.BC8.PE_R.Indx36 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTGTCCGGCTTTTTTTTTTTTTTTTTTTTV | |||
T20V.id37 | T20V.BC8.PE_R.Indx37 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTAAGGAATCTTTTTTTTTTTTTTTTTTTTV | |||
T20V.id38 | T20V.BC8.PE_R.Indx38 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCATCGTAGTTTTTTTTTTTTTTTTTTTTV | |||
T20V.id39 | T20V.BC8.PE_R.Indx39 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGTGTTGAGTTTTTTTTTTTTTTTTTTTTV | |||
T20V.id40 | T20V.BC8.PE_R.Indx40 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTATGTATCCTTTTTTTTTTTTTTTTTTTTV | |||
T20V.id41 | T20V.BC8.PE_R.Indx41 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTGACAGGTTTTTTTTTTTTTTTTTTTTTV | |||
T20V.id42 | T20V.BC8.PE_R.Indx42 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTACGCCAATTTTTTTTTTTTTTTTTTTTV | |||
T20V.id43 | T20V.BC8.PE_R.Indx43 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGAAGGCAATTTTTTTTTTTTTTTTTTTTV | |||
T20V.id44 | T20V.BC8.PE_R.Indx44 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCACCCGAGTTTTTTTTTTTTTTTTTTTTV | |||
T20V.id45 | T20V.BC8.PE_R.Indx45 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGGTGTGTCTTTTTTTTTTTTTTTTTTTTV | |||
T20V.id46 | T20V.BC8.PE_R.Indx46 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTCTTGTGCTTTTTTTTTTTTTTTTTTTTV | |||
T20V.id47 | T20V.BC8.PE_R.Indx47 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTAGTACCTGTTTTTTTTTTTTTTTTTTTTV | |||
T20V.id48 | T20V.BC8.PE_R.Indx48 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTAGCGTCCTTTTTTTTTTTTTTTTTTTTTV | |||
T20V.id49 | T20V.BC8.PE_R.Indx49 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTGAACTGTTTTTTTTTTTTTTTTTTTTTV | |||
T20V.id50 | T20V.BC8.PE_R.Indx50 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCAATGCTCTTTTTTTTTTTTTTTTTTTTV | |||
T20V.id51 | T20V.BC8.PE_R.Indx51 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGTTAGGCGTTTTTTTTTTTTTTTTTTTTV | |||
T20V.id52 | T20V.BC8.PE_R.Indx52 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTAGACGCTTTTTTTTTTTTTTTTTTTTTV | |||
T20V.id53 | T20V.BC8.PE_R.Indx53 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCTGGTGTGTTTTTTTTTTTTTTTTTTTTV | |||
T20V.id54 | T20V.BC8.PE_R.Indx54 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTAGCGGTAATTTTTTTTTTTTTTTTTTTTV | |||
T20V.id55 | T20V.BC8.PE_R.Indx55 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCCTTAAGTTTTTTTTTTTTTTTTTTTTTV | |||
T20V.id56 | T20V.BC8.PE_R.Indx56 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGCGGTTTCTTTTTTTTTTTTTTTTTTTTV | |||
T20V.id57 | T20V.BC8.PE_R.Indx57 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTAAGCCTAGTTTTTTTTTTTTTTTTTTTTV | |||
T20V.id58 | T20V.BC8.PE_R.Indx58 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTAGCCAGGTTTTTTTTTTTTTTTTTTTTV | |||
T20V.id59 | T20V.BC8.PE_R.Indx59 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGTGGTAAATTTTTTTTTTTTTTTTTTTTV | |||
T20V.id60 | T20V.BC8.PE_R.Indx60 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCACATAGGTTTTTTTTTTTTTTTTTTTTV | |||
T20V.id61 | T20V.BC8.PE_R.Indx61 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGCCGGTGATTTTTTTTTTTTTTTTTTTTV | |||
T20V.id62 | T20V.BC8.PE_R.Indx62 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCCTAAACCTTTTTTTTTTTTTTTTTTTTV | |||
T20V.id63 | T20V.BC8.PE_R.Indx63 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCGTGTCCCTTTTTTTTTTTTTTTTTTTTV | |||
T20V.id64 | T20V.BC8.PE_R.Indx64 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTAGGGTAGGTTTTTTTTTTTTTTTTTTTTV | |||
T20V.id65 | T20V.BC8.PE_R.Indx65 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGGGAGATGTTTTTTTTTTTTTTTTTTTTV | |||
T20V.id66 | T20V.BC8.PE_R.Indx66 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTAGGAAGTATTTTTTTTTTTTTTTTTTTTV | |||
T20V.id67 | T20V.BC8.PE_R.Indx67 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGGGCATGCTTTTTTTTTTTTTTTTTTTTV | |||
T20V.id68 | T20V.BC8.PE_R.Indx68 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGTAGGTACTTTTTTTTTTTTTTTTTTTTV | |||
T20V.id69 | T20V.BC8.PE_R.Indx69 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGCTGGATTTTTTTTTTTTTTTTTTTTTTV | |||
T20V.id70 | T20V.BC8.PE_R.Indx70 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTGCTCCCTTTTTTTTTTTTTTTTTTTTTV | |||
T20V.id71 | T20V.BC8.PE_R.Indx71 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTAAGCGCCCTTTTTTTTTTTTTTTTTTTTV | |||
T20V.id72 | T20V.BC8.PE_R.Indx72 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCGACGGATTTTTTTTTTTTTTTTTTTTTV | |||
T20V.id73 | T20V.BC8.PE_R.Indx73 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGGATAGCGTTTTTTTTTTTTTTTTTTTTV | |||
T20V.id74 | T20V.BC8.PE_R.Indx74 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGCCCAGTGTTTTTTTTTTTTTTTTTTTTV | |||
T20V.id75 | T20V.BC8.PE_R.Indx75 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGTGAAGACTTTTTTTTTTTTTTTTTTTTV | |||
T20V.id76 | T20V.BC8.PE_R.Indx76 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTAATCAGGATTTTTTTTTTTTTTTTTTTTV | |||
T20V.id77 | T20V.BC8.PE_R.Indx77 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTATGCTTCTTTTTTTTTTTTTTTTTTTTTV | |||
T20V.id78 | T20V.BC8.PE_R.Indx78 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCCAAGCAGTTTTTTTTTTTTTTTTTTTTV | |||
T20V.id79 | T20V.BC8.PE_R.Indx79 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGAAGTCGTTTTTTTTTTTTTTTTTTTTTV | |||
T20V.id80 | T20V.BC8.PE_R.Indx80 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTCGCCGCATTTTTTTTTTTTTTTTTTTTV | |||
T20V.id81 | T20V.BC8.PE_R.Indx81 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTCAGTTACTTTTTTTTTTTTTTTTTTTTV | |||
T20V.id82 | T20V.BC8.PE_R.Indx82 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTAACGTGCTTTTTTTTTTTTTTTTTTTTV | |||
T20V.id83 | T20V.BC8.PE_R.Indx83 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTGTAGGATTTTTTTTTTTTTTTTTTTTTV | |||
T20V.id84 | T20V.BC8.PE_R.Indx84 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTAGCACATCTTTTTTTTTTTTTTTTTTTTV | |||
T20V.id85 | T20V.BC8.PE_R.Indx85 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGTGTCTTGTTTTTTTTTTTTTTTTTTTTV | |||
T20V.id86 | T20V.BC8.PE_R.Indx86 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTGCAACCATTTTTTTTTTTTTTTTTTTTV | |||
T20V.id87 | T20V.BC8.PE_R.Indx87 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCACTTGTCTTTTTTTTTTTTTTTTTTTTV | |||
T20V.id88 | T20V.BC8.PE_R.Indx88 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGAGTTTGGTTTTTTTTTTTTTTTTTTTTV | |||
T20V.id89 | T20V.BC8.PE_R.Indx89 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGTGCTGGATTTTTTTTTTTTTTTTTTTTV | |||
T20V.id90 | T20V.BC8.PE_R.Indx90 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGAGAGCGCTTTTTTTTTTTTTTTTTTTTV | |||
T20V.id91 | T20V.BC8.PE_R.Indx91 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCCGAACATTTTTTTTTTTTTTTTTTTTTV | |||
T20V.id92 | T20V.BC8.PE_R.Indx92 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTACTATGCGTTTTTTTTTTTTTTTTTTTTV | |||
T20V.id93 | T20V.BC8.PE_R.Indx93 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTTAGCACTTTTTTTTTTTTTTTTTTTTTV | |||
T20V.id94 | T20V.BC8.PE_R.Indx94 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTATGTCCATTTTTTTTTTTTTTTTTTTTTV | |||
T20V.id95 | T20V.BC8.PE_R.Indx95 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCCCTGCCTTTTTTTTTTTTTTTTTTTTTV | |||
T20V.id96 | T20V.BC8.PE_R.Indx96 | /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTTGTCAGCTTTTTTTTTTTTTTTTTTTTV | |||
N2.id1 | PCR_R.N2Ind1 | CAAGCAGAAGACGGCATACGAGATCGTGATCTCGGCATTCCTGCTGAACCGCTCTT | CAAGCAGAAGACGGCATACGAGAT | CGTGAT | CTCGGCATTCCTGCTGAACCGCTCTT |
N2.id2 | PCR_R.N2Ind2 | CAAGCAGAAGACGGCATACGAGATACATCGCTCGGCATTCCTGCTGAACCGCTCTT | CAAGCAGAAGACGGCATACGAGAT | ACATCG | CTCGGCATTCCTGCTGAACCGCTCTT |
N2.id3 | PCR_R.N2Ind3 | CAAGCAGAAGACGGCATACGAGATGCCTAACTCGGCATTCCTGCTGAACCGCTCTT | CAAGCAGAAGACGGCATACGAGAT | GCCTAA | CTCGGCATTCCTGCTGAACCGCTCTT |
N2.id4 | PCR_R.N2Ind4 | CAAGCAGAAGACGGCATACGAGATTGGTCACTCGGCATTCCTGCTGAACCGCTCTT | CAAGCAGAAGACGGCATACGAGAT | TGGTCA | CTCGGCATTCCTGCTGAACCGCTCTT |
N2.id5 | PCR_R.N2Ind5 | CAAGCAGAAGACGGCATACGAGATCACTGTCTCGGCATTCCTGCTGAACCGCTCTT | CAAGCAGAAGACGGCATACGAGAT | CACTGT | CTCGGCATTCCTGCTGAACCGCTCTT |
N2.id6 | PCR_R.N2Ind6 | CAAGCAGAAGACGGCATACGAGATATTGGCCTCGGCATTCCTGCTGAACCGCTCT | CAAGCAGAAGACGGCATACGAGAT | ATTGGC | CTCGGCATTCCTGCTGAACCGCTCTT |
N2.id7 | PCR_R.N2Ind7 | CAAGCAGAAGACGGCATACGAGATGATCTGCTCGGCATTCCTGCTGAACCGCTCTT | CAAGCAGAAGACGGCATACGAGAT | GATCTG | CTCGGCATTCCTGCTGAACCGCTCTT |
N2.id8 | PCR_R.N2Ind8 | CAAGCAGAAGACGGCATACGAGATTCAAGTCTCGGCATTCCTGCTGAACCGCTCTT | CAAGCAGAAGACGGCATACGAGAT | TCAAGT | CTCGGCATTCCTGCTGAACCGCTCTT |
N2.id9 | PCR_R.N2Ind9 | CAAGCAGAAGACGGCATACGAGATCTGATCCTCGGCATTCCTGCTGAACCGCTCTT | CAAGCAGAAGACGGCATACGAGAT | CTGATC | CTCGGCATTCCTGCTGAACCGCTCTT |
N2.id10 | PCR_R.N2Ind10 | CAAGCAGAAGACGGCATACGAGATAAGCTACTCGGCATTCCTGCTGAACCGCTCTT | CAAGCAGAAGACGGCATACGAGAT | AAGCTA | CTCGGCATTCCTGCTGAACCGCTCTT |
N2.id11 | PCR_R.N2Ind11 | CAAGCAGAAGACGGCATACGAGATGTAGCCCTCGGCATTCCTGCTGAACCGCTCTT | CAAGCAGAAGACGGCATACGAGAT | GTAGCC | CTCGGCATTCCTGCTGAACCGCTCTT |
N2.id12 | PCR_R.N2Ind12 | CAAGCAGAAGACGGCATACGAGATTACAAGCTCGGCATTCCTGCTGAACCGCTCTT | CAAGCAGAAGACGGCATACGAGAT | TACAAG | CTCGGCATTCCTGCTGAACCGCTCTT |
N2.id13 | PCR_R.N2Ind13 | CAAGCAGAAGACGGCATACGAGAT | CTCGGCATTCCTGCTGAACCGCTCTT | ||
N2.id14 | PCR_R.N2Ind14 | CAAGCAGAAGACGGCATACGAGAT | CTCGGCATTCCTGCTGAACCGCTCTT | ||
N2.id81 | PCR_R.N2Ind81 | CAAGCAGAAGACGGCATACGAGATCCAGGTCTCGGCATTCCTGCTGAACCGCTCTT | CAAGCAGAAGACGGCATACGAGAT | CCAGGT | CTCGGCATTCCTGCTGAACCGCTCTT |
N2.id82 | PCR_R.N2Ind82 | CAAGCAGAAGACGGCATACGAGATCGTTTGCTCGGCATTCCTGCTGAACCGCTCTT | CAAGCAGAAGACGGCATACGAGAT | CGTTTG | CTCGGCATTCCTGCTGAACCGCTCTT |
N2.id83 | PCR_R.N2Ind83 | CAAGCAGAAGACGGCATACGAGATGACCACCTCGGCATTCCTGCTGAACCGCTCTT | CAAGCAGAAGACGGCATACGAGAT | GACCAC | CTCGGCATTCCTGCTGAACCGCTCTT |
N2.id84 | PCR_R.N2Ind84 | CAAGCAGAAGACGGCATACGAGATACAAGACTCGGCATTCCTGCTGAACCGCTCTT | CAAGCAGAAGACGGCATACGAGAT | ACAAGA | CTCGGCATTCCTGCTGAACCGCTCTT |
N2.id85 | PCR_R.N2Ind85 | CAAGCAGAAGACGGCATACGAGATACCGCACTCGGCATTCCTGCTGAACCGCTCTT | CAAGCAGAAGACGGCATACGAGAT | ACCGCA | CTCGGCATTCCTGCTGAACCGCTCTT |
N2.id86 | PCR_R.N2Ind86 | CAAGCAGAAGACGGCATACGAGATTGGGTACTCGGCATTCCTGCTGAACCGCTCTT | CAAGCAGAAGACGGCATACGAGAT | TGGGTA | CTCGGCATTCCTGCTGAACCGCTCTT |
N2.id87 | PCR_R.N2Ind87 | CAAGCAGAAGACGGCATACGAGATATTCCGCTCGGCATTCCTGCTGAACCGCTCTT | CAAGCAGAAGACGGCATACGAGAT | ATTCCG | CTCGGCATTCCTGCTGAACCGCTCTT |
N2.id88 | PCR_R.N2Ind88 | CAAGCAGAAGACGGCATACGAGATGAATGTCTCGGCATTCCTGCTGAACCGCTCTT | CAAGCAGAAGACGGCATACGAGAT | GAATGT | CTCGGCATTCCTGCTGAACCGCTCTT |
N2.id89 | PCR_R.N2Ind89 | CAAGCAGAAGACGGCATACGAGATGCTGATCTCGGCATTCCTGCTGAACCGCTCTT | CAAGCAGAAGACGGCATACGAGAT | GCTGAT | CTCGGCATTCCTGCTGAACCGCTCTT |
N2.id90 | PCR_R.N2Ind90 | CAAGCAGAAGACGGCATACGAGATAGTGCTCTCGGCATTCCTGCTGAACCGCTCTT | CAAGCAGAAGACGGCATACGAGAT | AGTGCT | CTCGGCATTCCTGCTGAACCGCTCTT |
N2.id91 | PCR_R.N2Ind91 | CAAGCAGAAGACGGCATACGAGATCAGGGACTCGGCATTCCTGCTGAACCGCTCTT | CAAGCAGAAGACGGCATACGAGAT | CAGGGA | CTCGGCATTCCTGCTGAACCGCTCTT |
N2.id92 | PCR_R.N2Ind92 | CAAGCAGAAGACGGCATACGAGATCATGCGCTCGGCATTCCTGCTGAACCGCTCTT | CAAGCAGAAGACGGCATACGAGAT | CATGCG | CTCGGCATTCCTGCTGAACCGCTCTT |
N2.id93 | PCR_R.N2Ind93 | CAAGCAGAAGACGGCATACGAGATTGCCTACTCGGCATTCCTGCTGAACCGCTCTT | CAAGCAGAAGACGGCATACGAGAT | TGCCTA | CTCGGCATTCCTGCTGAACCGCTCTT |
N2.id94 | PCR_R.N2Ind94 | CAAGCAGAAGACGGCATACGAGATCTATACCTCGGCATTCCTGCTGAACCGCTCTT | CAAGCAGAAGACGGCATACGAGAT | CTATAC | CTCGGCATTCCTGCTGAACCGCTCTT |
N2.id95 | PCR_R.N2Ind95 | CAAGCAGAAGACGGCATACGAGATCCGAGTCTCGGCATTCCTGCTGAACCGCTCTT | CAAGCAGAAGACGGCATACGAGAT | CCGAGT | CTCGGCATTCCTGCTGAACCGCTCTT |
N2.id96 | PCR_R.N2Ind96 | CAAGCAGAAGACGGCATACGAGATACCTGCCTCGGCATTCCTGCTGAACCGCTCTT | CAAGCAGAAGACGGCATACGAGAT | ACCTGC | CTCGGCATTCCTGCTGAACCGCTCTT |
PGR_PCR_R | CAAGCAGAAGACGGCATACGAGATCTCGGCATTCCTGCTGAACCGCTCTT | CGAGAT | |||
PGR_PCR_Rb | PGR_PCR_Rmod | CAAGCAGAAGACGGCATACGAGATCTCGGCATTCCTGCTGAACCGCTCTT | /5Phos/CAAGCAGAAGACGGCATACGAGATCTCGGCATTCCTGCTGAACCGCTCTT | ||
ILMN_PCR_Rb | ILMN_PCR_Rmod | CAAGCAGAAGACGGCATACGAGAT | /5Phos/CAAGCAGAAGACGGCATACGAGAT |
IVT 5' primer sequence[edit]
- The 5' adaptors list
Primer full name | Primer full sequence | PB_PCR_F Complementary Seq | Random Sequence |
RL.IVT.N6 | ACACTCTTTCCCTACACGACGNNNNNN | ACACTCTTTCCCTACACGACG | NNNNNN |
RL.IVT.N9 | ACACTCTTTCCCTACACGACGNNNNNNNNN | ACACTCTTTCCCTACACGACG | NNNNNNNNN |
IVT 3' primer sequence[edit]
- The 3' adaptors list
Primer full name | Primer full sequence | T20 Complementary Seq | Novel T7 Seq |
RL.Add.T7 | CTTTAATTATGCTGAGTGATATCCCCGTAAGGACGACTTGGCGAGAA | GCATTCCTGCTGAACCGCTCTT | CTTTAATTATGCTGAGTGATATCCC |
MARS-Seq/STRT-Seq/Smart-Seq2/Quartz-Seq Primer Sequences[edit]
Protocol | Primer full name | Primer full sequence | Modification | Barcode Seq |
MARS-Seq | MARS_T20N_id01 | CGATTGAGGCCGGTAATACGACTCACTATAGGGGCGACGTGTGCTCTTCCGATCTCGTGATNNNNTTTTTTTTTTTTTTTTTTTTN | N/A | CGTGAT |
MARS-Seq | MARS_T20N_id02 | CGATTGAGGCCGGTAATACGACTCACTATAGGGGCGACGTGTGCTCTTCCGATCTACATCGNNNNTTTTTTTTTTTTTTTTTTTTN | N/A | ACATCG |
MARS-Seq | MARS_T20N_id03 | CGATTGAGGCCGGTAATACGACTCACTATAGGGGCGACGTGTGCTCTTCCGATCTGCCTAANNNNTTTTTTTTTTTTTTTTTTTTN | N/A | GCCTAA |
MARS-Seq | MARS_T20N_id04 | CGATTGAGGCCGGTAATACGACTCACTATAGGGGCGACGTGTGCTCTTCCGATCTTGGTCANNNNTTTTTTTTTTTTTTTTTTTTN | N/A | TGGTCA |
MARS-Seq | MARS_T20N_id05 | CGATTGAGGCCGGTAATACGACTCACTATAGGGGCGACGTGTGCTCTTCCGATCTCACTGTNNNNTTTTTTTTTTTTTTTTTTTTN | N/A | CACTGT |
MARS-Seq | MARS_T20N_id06 | CGATTGAGGCCGGTAATACGACTCACTATAGGGGCGACGTGTGCTCTTCCGATCTATTGGCNNNNTTTTTTTTTTTTTTTTTTTTN | N/A | ATTGGC |
MARS-Seq | MARS_T20N_id07 | CGATTGAGGCCGGTAATACGACTCACTATAGGGGCGACGTGTGCTCTTCCGATCTGATCTGNNNNTTTTTTTTTTTTTTTTTTTTN | N/A | GATCTG |
MARS-Seq | MARS_T20N_id08 | CGATTGAGGCCGGTAATACGACTCACTATAGGGGCGACGTGTGCTCTTCCGATCTTCAAGTNNNNTTTTTTTTTTTTTTTTTTTTN | N/A | TCAAGT |
MARS-Seq | MARS_T20N_id09 | CGATTGAGGCCGGTAATACGACTCACTATAGGGGCGACGTGTGCTCTTCCGATCTCTGATCNNNNTTTTTTTTTTTTTTTTTTTTN | N/A | CTGATC |
MARS-Seq | MARS_T20N_id10 | CGATTGAGGCCGGTAATACGACTCACTATAGGGGCGACGTGTGCTCTTCCGATCTAAGCTANNNNTTTTTTTTTTTTTTTTTTTTN | N/A | AAGCTA |
MARS-Seq | MARS_RT_2 | TCTAGCCTTCTCGCAGCACATC | N/A | |
MARS-Seq | P5_Rd1_PCR_F | AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT | N/A | |
MARS-Seq | P7_Rd2_PCR_R | CAAGCAGAAGACGGCATACGAGATGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT | N/A | |
MARS-Seq | MARS_lig_NNNX4_ix1 | /5Phos/GACTNNNAGATCGGAAGAGCGTCGTGTAG/3SpC3/ | 5Phos/3SpC3 | GACT |
MARS-Seq | MARS_lig_NNNX4_ix2 | /5Phos/CATGNNNAGATCGGAAGAGCGTCGTGTAG/3SpC3/ | 5Phos/3SpC3 | CATG |
MARS-Seq | MARS_lig_NNNX4_ix3 | /5Phos/CCAANNNAGATCGGAAGAGCGTCGTGTAG/3SpC3/ | 5Phos/3SpC3 | CCAA |
MARS-Seq | MARS_lig_NNNX4_ix4 | /5Phos/CTGTNNNAGATCGGAAGAGCGTCGTGTAG/3SpC3/ | 5Phos/3SpC3 | CTGT |
MARS-Seq | MARS_lig_NNNX4_ix5 | /5Phos/GTAGNNNAGATCGGAAGAGCGTCGTGTAG/3SpC3/ | 5Phos/3SpC3 | GTAG |
MARS-Seq | MARS_lig_NNNX4_ix6 | /5Phos/TGATNNNAGATCGGAAGAGCGTCGTGTAG/3SpC3/ | 5Phos/3SpC3 | TGAT |
Smart-seq2 | Smart2-T30VN | AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTVN | N/A | |
Smart-seq2 | Smart2_ISPCR | AAGCAGTGGTATCAACGCAGAGT | N/A | |
Smart-Seq2 | Smart2-TSO | AAGCAGTGGTATCAACGCAGAGTACATrGrG+G | rGrG+G (LNA) | |
UMI-STRT-Seq | C1-P1-RNA-TSO | Bio-AAUGAUACGGCGACCACCGAUNNNNNGGG | RNA | |
UMI-STRT-Seq | C1-P1-T31 | Bio-AATGATACGGCGACCACCGATCGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | ||
UMI-STRT-Seq | C1-P1-PCR-2 | Bio-GAATGATACGGCGACCACCGAT | ||
UMI-STRT-Seq | C1-TN5-U | PHO-CTGTCTCTTATACACATCTGACGC | ||
Quartz-Seq | RT primer (WTA) | TATAGAATTCGCGGCCGCTCGCGATAATACGACTCACTATAGGGCGTTTTTTTTTTTTTTTTTTTTTTTT | N/A | |
Quartz-Seq | Tagging primer | TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTT | N/A | |
Quartz-Seq | Suppression primer | (NH2)-GTATAGAATTCGCGGCCGCTCGCGAT |
NexteraXT Primers[edit]
Primer | Sequence | Index Sequence |
NexteraXT.N7id01 | CAAGCAGAAGACGGCATACGAGATCGTGATGTCTCGTGGGCTCGG | CGTGAT |
NexteraXT.N7id02 | CAAGCAGAAGACGGCATACGAGATACATCGGTCTCGTGGGCTCGG | ACATCG |
NexteraXT.N7id03 | CAAGCAGAAGACGGCATACGAGATCGACTCGTCTCGTGGGCTCGG | CGACTC |
NexteraXT.N7id04 | CAAGCAGAAGACGGCATACGAGATTGGTCAGTCTCGTGGGCTCGG | TGGTCA |
NexteraXT.N7id05 | CAAGCAGAAGACGGCATACGAGATCACTGTGTCTCGTGGGCTCGG | CACTGT |
NexteraXT.N7id06 | CAAGCAGAAGACGGCATACGAGATATTGGCGTCTCGTGGGCTCGG | ATTGGC |
NexteraXT.N7id07 | CAAGCAGAAGACGGCATACGAGATGATCTGGTCTCGTGGGCTCGG | GATCTG |
NexteraXT.N7id08 | CAAGCAGAAGACGGCATACGAGATTCAAGTGTCTCGTGGGCTCGG | TCAAGT |
NexteraXT.N7id09 | CAAGCAGAAGACGGCATACGAGATGAGCTCGTCTCGTGGGCTCGG | GAGCTC |
NexteraXT.N7id10 | CAAGCAGAAGACGGCATACGAGATAAGCTAGTCTCGTGGGCTCGG | AAGCTA |
NexteraXT.N7id11 | CAAGCAGAAGACGGCATACGAGATGTAGCCGTCTCGTGGGCTCGG | GTAGCC |
NexteraXT.N7id12 | CAAGCAGAAGACGGCATACGAGATTACAAGGTCTCGTGGGCTCGG | TACAAG |
NexteraXT.N7id13 | CAAGCAGAAGACGGCATACGAGATTCATGGGTCTCGTGGGCTCGG | TCATGG |
NexteraXT.N7id14 | CAAGCAGAAGACGGCATACGAGATTGTCTTGTCTCGTGGGCTCGG | TGTCTT |
NexteraXT.N7id15 | CAAGCAGAAGACGGCATACGAGATAGGAAGGTCTCGTGGGCTCGG | AGGAAG |
NexteraXT.N7id16 | CAAGCAGAAGACGGCATACGAGATAACCCCGTCTCGTGGGCTCGG | AACCCC |
NexteraXT.N7id17 | CAAGCAGAAGACGGCATACGAGATGATGAGGTCTCGTGGGCTCGG | GATGAG |
NexteraXT.N7id18 | CAAGCAGAAGACGGCATACGAGATTGAACTGTCTCGTGGGCTCGG | TGAACT |
NexteraXT.N7id19 | CAAGCAGAAGACGGCATACGAGATTGCGTCGTCTCGTGGGCTCGG | TGCGTC |
NexteraXT.N7id20 | CAAGCAGAAGACGGCATACGAGATGACAGGGTCTCGTGGGCTCGG | GACAGG |
NexteraXT.N7id21 | CAAGCAGAAGACGGCATACGAGATGGGTTGGTCTCGTGGGCTCGG | GGGTTG |
NexteraXT.N7id22 | CAAGCAGAAGACGGCATACGAGATTCCGAGGTCTCGTGGGCTCGG | TCCGAG |
NexteraXT.N7id23 | CAAGCAGAAGACGGCATACGAGATTTTCGAGTCTCGTGGGCTCGG | TTTCGA |
NexteraXT.N7id24 | CAAGCAGAAGACGGCATACGAGATGCGAATGTCTCGTGGGCTCGG | GCGAAT |
NexteraXT.S501 | AATGATACGGCGACCACCGAGATCTACACTAGATCGCTCGTCGGCAGCGTC | TAGATCGC |
Sequencing Primers[edit]
Primer full name | Primer full sequence | Protocol | Read |
totoRNAseq Read 1 v2 | CCACCGAGATCTACACTCTTTCCCTACACGACG | totoRNAseq | Read1 |
N2RevSeq2 | CTCGGCATTCCTGCTGAACCGCTCTTCCGATCT | totoRNAseq | Read2 |
N2IndSeq | AAGAGCGGTTCAGCAGGAATGCCGAG | totoRNAseq | N2 index Read |
STRT-SEQ v3 | GATCTAAGCAGTGGTATCAACGCAGAGTACGGG | totoRNAseq | Read1 |