Hosuk:LabNotesMDA/2013-7-22: Difference between revisions

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*[[File:qPCR_Result_MDAv9.1_2013-07-22_fig1.png|400px]]
*[[File:qPCR_Result_MDAv9.1_2013-07-22_fig1.png|450px]]
*[[File:qPCR_Result_MDAv9.1_2013-07-22_fig2_Table.png|300px]]
*[[File:qPCR_Result_MDAv9.1_2013-07-22_fig2_Table.png|250px]]
*[[File:qPCR_Result_MDAv9.1_2013-07-22_fig3_History.png|650px]]
*[[File:qPCR_Result_MDAv9.1_2013-07-22_fig3_History.png|650px]]

Latest revision as of 18:52, 26 July 2013


MDA with PGP1F, 9th, Dev v9.1[edit]

  • Use new device (v9.1)
  • Same experiment to 07/18, but try to run MDA in ALS chamber after transferring solution into compartments.


Experiment[edit]

  • NS valve didn’t work properly but the reaction was kept going. (8hr reaction)


Result[edit]

  • Not bad, considering bad experiment condition
  • Ch25 is the reaction in ALS chamber.
    • The amount of amplicon was estimated 483.26pg/uL, which is 724.89pg in total.
    • While transferring ALS+Cell solution into compartment with air, the remained solution was kept drying (evaporation by air)
    • Thus using H2O would be better than air.