Hosuk:LabNotesMDA/2013-7-22: Difference between revisions
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*[[File:qPCR_Result_MDAv9.1_2013-07-22_fig1.png|450px]] | *[[File:qPCR_Result_MDAv9.1_2013-07-22_fig1.png|450px]] | ||
*[[File:qPCR_Result_MDAv9.1_2013-07-22_fig2_Table.png| | *[[File:qPCR_Result_MDAv9.1_2013-07-22_fig2_Table.png|250px]] | ||
*[[File:qPCR_Result_MDAv9.1_2013-07-22_fig3_History.png| | *[[File:qPCR_Result_MDAv9.1_2013-07-22_fig3_History.png|650px]] |
Latest revision as of 18:52, 26 July 2013
MDA with PGP1F, 9th, Dev v9.1[edit]
- Use new device (v9.1)
- Same experiment to 07/18, but try to run MDA in ALS chamber after transferring solution into compartments.
Experiment[edit]
- NS valve didn’t work properly but the reaction was kept going. (8hr reaction)
Result[edit]
- Not bad, considering bad experiment condition
- Ch25 is the reaction in ALS chamber.
- The amount of amplicon was estimated 483.26pg/uL, which is 724.89pg in total.
- While transferring ALS+Cell solution into compartment with air, the remained solution was kept drying (evaporation by air)
- Thus using H2O would be better than air.