Hosuk:LabNotesMDA/2013-8-27: Difference between revisions
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[[File:qPCR_Result_MDAv9.1_2013-08-26_QTable.png|550px]] | |||
[[File:qPCR_Result_MDAv9.1_2013-08-26_HistoryRecent.png|500px]] | [[File:qPCR_Result_MDAv9.1_2013-08-26_HistoryRecent.png|500px]] | ||
====Next plan==== | |||
*Tagmentation | |||
**Use 3uL extraction. | |||
**2uL instead was much easier and precise due to the line in pipette tip (10uL tip). | |||
**2uL sample + 3uL ALS(400 uM KOH)+ 3uL NS |
Latest revision as of 19:49, 27 August 2013
MDA with PGP1F, 14th, Dev v9.1[edit]
- Use mineral oil in valve lines, Water in valves for cell capture and mineral oil for rest of valves
- 10hr reaction
Result[edit]
- Result : Not bad…but
- Pushing water doesn’t look good. Chromosome wasn’t pushed completely.
- Ch 1, 2, 3 had not enough template, and MDA product.
- Pushing air and run MDA in denature chamber…
File:QPCR Result MDAv9.1 2013-08-26 QTable.png File:QPCR Result MDAv9.1 2013-08-26 HistoryRecent.png
Next plan[edit]
- Tagmentation
- Use 3uL extraction.
- 2uL instead was much easier and precise due to the line in pipette tip (10uL tip).
- 2uL sample + 3uL ALS(400 uM KOH)+ 3uL NS