Daniel:Notebook/HiResChrPaint/2013-9-30: Difference between revisions

From ZhangLabWiki
Jump to navigation Jump to search
>Djacobse
(Created page with "=Fibroblast Culture= Back to Calendar Split passage 9 cells into 3 culture dishes, also prepped 5 plates fo...")
 
>Djacobse
 
(One intermediate revision by the same user not shown)
Line 11: Line 11:
  3. Aspirated media and added 1 mL fresh DMEM
  3. Aspirated media and added 1 mL fresh DMEM
  4. Incubated overnight
  4. Incubated overnight
=FISH ([[Daniel:Notebook/HiResChrPaint/2013-9-27|Started 9/27/2013]])=
==Microscopy==
Performed microscopy on samples from 9/27 using Olympus microscope upstairs.
===Results===
'''Control'''
{|class="wikitable"
|-
|[[Image:Dan_exp_overlay_bfdapigfp_40x_FISH_20130930.png|800px]]
|-
|40x magnification; BF,DAPI,GFP
|}
{|class="wikitable"
|-
|[[Image:Dan_exp_overlay_bfdapitxred_10x_FISH_20130930.png|500px]]
|[[Image:Dan_exp_overlay_bfdapitxred_20x_FISH_20130930.png|500px]]
|-
|10x magnification; BF,DAPI,TXRED
|20x magnification; BF,DAPI,TXRED
|-
|[[Image:Dan_exp_overlay_bfdapitxred_40xFISH_20130930.png|500px]]
|[[Image:Overlay-all_FISH_20130930.png|500px]]
|-
|40x magnification; BF,DAPI,TXRED
|40x magnification; BF,DAPI,TXRED,GFP
|}

Latest revision as of 19:08, 3 October 2013

Fibroblast Culture[edit]

Back to Calendar

Split passage 9 cells into 3 culture dishes, also prepped 5 plates for fixing/hybridization.

Prepping cells for hybridization[edit]

1. Added 100 uL of cell suspension (after step 5) to center of petri dish
2. Incubated for ~9 hours
3. Aspirated media and added 1 mL fresh DMEM
4. Incubated overnight

FISH (Started 9/27/2013)[edit]

Microscopy[edit]

Performed microscopy on samples from 9/27 using Olympus microscope upstairs.

Results[edit]

Control

File:Dan exp overlay bfdapigfp 40x FISH 20130930.png
40x magnification; BF,DAPI,GFP


File:Dan exp overlay bfdapitxred 10x FISH 20130930.png File:Dan exp overlay bfdapitxred 20x FISH 20130930.png
10x magnification; BF,DAPI,TXRED 20x magnification; BF,DAPI,TXRED
File:Dan exp overlay bfdapitxred 40xFISH 20130930.png File:Overlay-all FISH 20130930.png
40x magnification; BF,DAPI,TXRED 40x magnification; BF,DAPI,TXRED,GFP