Hosuk:LabNotes/2013-10-9: Difference between revisions
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>Hosuki78 (Created page with "*LabNote ===1st Rolonies and ACTB Gene detection in 1st Rolonies - Tile scan, 2k x 2k resolution images with Confocal === ====Parameters==== *488nm laser...") |
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*[[Hosuk:Lab_Notes|LabNote]] | *[[Hosuk:Lab_Notes|LabNote]] | ||
===1st Rolonies and ACTB Gene detection in 1st Rolonies | ===1st Rolonies and ACTB Gene detection in 1st Rolonies === | ||
*Tile scan, 2k x 2k resolution images with Confocal | |||
====Parameters==== | ====Parameters==== | ||
*488nm laser for ACTB target probe (ATTO488), 638nm for 1st Rolonies (Cy5) | *488nm laser for ACTB target probe (ATTO488), 638nm for 1st Rolonies (Cy5) | ||
*20x obj., zoom = 1.00, Pinhole = 2.00 | *20x obj., zoom = 1.00, Pinhole = 2.00 | ||
*Resolution format : 2048 x 2048 Format | *Resolution format : 2048 x 2048 Format (8192 x 8192 --> Pixel size : 70.96nm x 70.96nm) | ||
*Field of view : | *Field of view : 581.25um x 581.25um | ||
*Pixel size : 284nm x 284nm | *Pixel size : 284nm x 284nm | ||
*PMT1 (ATTO488) : Gain 670V, Offset -2.00% | *PMT1 (ATTO488) : Gain 670V, Offset -2.00% | ||
*PMT2 (Cy5) : Gain 550V, Offset -2.00% | *PMT2 (Cy5) : Gain 550V, Offset -2.00% | ||
*Z stack : step size : 0.54um, Total 10.87um scan (21 images) | *Z stack : step size : 0.54um, Total 10.87um scan (21 images) | ||
*Tile size = 8 x 6 | *Tile size = 8 x 6 --> 48 tiles | ||
*Total scanning time : 3hr. 10min | *Total scanning time : 3hr. 10min | ||
====Result==== | |||
*Analysis is on going |
Latest revision as of 22:30, 10 October 2013
1st Rolonies and ACTB Gene detection in 1st Rolonies[edit]
- Tile scan, 2k x 2k resolution images with Confocal
Parameters[edit]
- 488nm laser for ACTB target probe (ATTO488), 638nm for 1st Rolonies (Cy5)
- 20x obj., zoom = 1.00, Pinhole = 2.00
- Resolution format : 2048 x 2048 Format (8192 x 8192 --> Pixel size : 70.96nm x 70.96nm)
- Field of view : 581.25um x 581.25um
- Pixel size : 284nm x 284nm
- PMT1 (ATTO488) : Gain 670V, Offset -2.00%
- PMT2 (Cy5) : Gain 550V, Offset -2.00%
- Z stack : step size : 0.54um, Total 10.87um scan (21 images)
- Tile size = 8 x 6 --> 48 tiles
- Total scanning time : 3hr. 10min
Result[edit]
- Analysis is on going