Hosuk:LabNotes/2013-10-17: Difference between revisions

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(Created page with "*LabNote ===Rolonies with fresh cells (96well plate)=== ====Goal==== *Produce rolonies with (relatively) fresh cells *Try invitrogen a-dUTP (from Dan) ...")
 
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*Produce rolonies with (relatively) fresh cells
*Produce rolonies with (relatively) fresh cells
*Try invitrogen a-dUTP (from Dan) and compare to the current (brown vial) anaspect's one
*Try invitrogen a-dUTP (from Dan) and compare to the current (brown vial) anaspect's one
*Check if there are many ACTB from these fresh cell samples.




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*Got PGP1F from Alan, the cells were thaw two days ago
*Got PGP1F from Alan, the cells were thaw two days ago
*Split cells (10/16) on 8 wells in 96 well plate, 2 Mattek  dishes
*Split cells (10/16) on 8 wells in 96 well plate, 2 Mattek  dishes
**40uL per each well (+100uL media), 1mL per each Mattek dish
*Fix cells (10/17) and proceed RT (9:30 pm)
*Fix cells (10/17) and proceed RT (9:30 pm)

Latest revision as of 04:37, 18 October 2013


Rolonies with fresh cells (96well plate)[edit]

Goal[edit]

  • Produce rolonies with (relatively) fresh cells
  • Try invitrogen a-dUTP (from Dan) and compare to the current (brown vial) anaspect's one
  • Check if there are many ACTB from these fresh cell samples.


Process[edit]

  • Got PGP1F from Alan, the cells were thaw two days ago
  • Split cells (10/16) on 8 wells in 96 well plate, 2 Mattek dishes
    • 40uL per each well (+100uL media), 1mL per each Mattek dish
  • Fix cells (10/17) and proceed RT (9:30 pm)