Hosuk:LabNotes/2013-10-17: Difference between revisions
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>Hosuki78 (Created page with "*LabNote ===Rolonies with fresh cells (96well plate)=== ====Goal==== *Produce rolonies with (relatively) fresh cells *Try invitrogen a-dUTP (from Dan) ...") |
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*Produce rolonies with (relatively) fresh cells | *Produce rolonies with (relatively) fresh cells | ||
*Try invitrogen a-dUTP (from Dan) and compare to the current (brown vial) anaspect's one | *Try invitrogen a-dUTP (from Dan) and compare to the current (brown vial) anaspect's one | ||
*Check if there are many ACTB from these fresh cell samples. | |||
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*Got PGP1F from Alan, the cells were thaw two days ago | *Got PGP1F from Alan, the cells were thaw two days ago | ||
*Split cells (10/16) on 8 wells in 96 well plate, 2 Mattek dishes | *Split cells (10/16) on 8 wells in 96 well plate, 2 Mattek dishes | ||
**40uL per each well (+100uL media), 1mL per each Mattek dish | |||
*Fix cells (10/17) and proceed RT (9:30 pm) | *Fix cells (10/17) and proceed RT (9:30 pm) |
Latest revision as of 04:37, 18 October 2013
Rolonies with fresh cells (96well plate)[edit]
Goal[edit]
- Produce rolonies with (relatively) fresh cells
- Try invitrogen a-dUTP (from Dan) and compare to the current (brown vial) anaspect's one
- Check if there are many ACTB from these fresh cell samples.
Process[edit]
- Got PGP1F from Alan, the cells were thaw two days ago
- Split cells (10/16) on 8 wells in 96 well plate, 2 Mattek dishes
- 40uL per each well (+100uL media), 1mL per each Mattek dish
- Fix cells (10/17) and proceed RT (9:30 pm)