Hosuk:LabNotes/2014-1-9: Difference between revisions

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>Hosuki78
(Created page with "*LabNote ===RT primer annealing specific position in mRNA : 2nd try, in MatTek Dish=== *PGP1F P13 in MatTek Dish : #1 for RT Primer D220, #2 for RT Primer...")
 
>Hosuki78
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===RT primer annealing specific position in mRNA : 2nd try, in MatTek Dish===
===RT primer annealing specific position in mRNA : 2nd try, in MatTek Dish===
*PGP1F P13 in MatTek Dish : #1 for RT Primer D220, #2 for RT Primer D1000 (01/07~01/09)
*PGP1F P13 in MatTek Dish : #1 for RT Primer D220, #2 for RT Primer D1000 (01/07~01/09)
====Result ====
====Result ====
*1st Rolony detection probe (Cy5) : count = 5135
*1st Rolony detection probe (Cy5) : count = 5135
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*Overlap count of RAB7A : count = 14
*Overlap count of RAB7A : count = 14
**''''Almost no RAB7A target in 1st Rolony…WHY?…'''
**''''Almost no RAB7A target in 1st Rolony…WHY?…'''
*Raw images
**[[Media:MIP_RTPrimer_RAB7A-2_Step2_1stRolony-Cy5.tif|D1000, 1st Rolony]]
**[[Media:MIP_RTPrimer_RAB7A-2_Step1_RAB7A-ATTO550.tif|D1000, RAB7A target detection]]
*[[File:MIP_RTPrimer_1stRolony_RAB7A_detection.png|800px]]

Latest revision as of 00:28, 20 January 2014

RT primer annealing specific position in mRNA : 2nd try, in MatTek Dish[edit]

  • PGP1F P13 in MatTek Dish : #1 for RT Primer D220, #2 for RT Primer D1000 (01/07~01/09)


Result[edit]

  • 1st Rolony detection probe (Cy5) : count = 5135
  • RAB7A detection probe (ATTO550) : count = 64
  • Overlap count of RAB7A : count = 14
    • 'Almost no RAB7A target in 1st Rolony…WHY?…