Hosuk:LabNotes/2014-1-9: Difference between revisions
Jump to navigation
Jump to search
>Hosuki78 (Created page with "*LabNote ===RT primer annealing specific position in mRNA : 2nd try, in MatTek Dish=== *PGP1F P13 in MatTek Dish : #1 for RT Primer D220, #2 for RT Primer...") |
>Hosuki78 No edit summary |
||
(One intermediate revision by the same user not shown) | |||
Line 3: | Line 3: | ||
===RT primer annealing specific position in mRNA : 2nd try, in MatTek Dish=== | ===RT primer annealing specific position in mRNA : 2nd try, in MatTek Dish=== | ||
*PGP1F P13 in MatTek Dish : #1 for RT Primer D220, #2 for RT Primer D1000 (01/07~01/09) | *PGP1F P13 in MatTek Dish : #1 for RT Primer D220, #2 for RT Primer D1000 (01/07~01/09) | ||
====Result ==== | ====Result ==== | ||
*1st Rolony detection probe (Cy5) : count = 5135 | *1st Rolony detection probe (Cy5) : count = 5135 | ||
Line 8: | Line 10: | ||
*Overlap count of RAB7A : count = 14 | *Overlap count of RAB7A : count = 14 | ||
**''''Almost no RAB7A target in 1st Rolony…WHY?…''' | **''''Almost no RAB7A target in 1st Rolony…WHY?…''' | ||
*Raw images | |||
**[[Media:MIP_RTPrimer_RAB7A-2_Step2_1stRolony-Cy5.tif|D1000, 1st Rolony]] | |||
**[[Media:MIP_RTPrimer_RAB7A-2_Step1_RAB7A-ATTO550.tif|D1000, RAB7A target detection]] | |||
*[[File:MIP_RTPrimer_1stRolony_RAB7A_detection.png|800px]] |
Latest revision as of 00:28, 20 January 2014
RT primer annealing specific position in mRNA : 2nd try, in MatTek Dish[edit]
- PGP1F P13 in MatTek Dish : #1 for RT Primer D220, #2 for RT Primer D1000 (01/07~01/09)
Result[edit]
- 1st Rolony detection probe (Cy5) : count = 5135
- RAB7A detection probe (ATTO550) : count = 64
- Overlap count of RAB7A : count = 14
- 'Almost no RAB7A target in 1st Rolony…WHY?…