Daniel:Notebook/HiResChrPaint/2014-2-17: Difference between revisions

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==Cot1 Labeling ([[Daniel:Notebook/HiResChrPaint/2014-2-14|Started 2/14/2014]])==
==Cot1 Labeling ([[Daniel:Notebook/HiResChrPaint/2014-2-14|Started 2/14/2014]])==
#Measure concentration of all samples in nanodrop
#Run on [[Daniel:Protocols/TBEgel|TBE gel]] to ensure correct size distribution
#Pool all high MW samples and all low MW samples


===Results===
===Results===
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'''Nanodrop Results'''
'''Nanodrop Results'''


{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext>
|- style="font-size:12pt" align="center"
| align="center" width="65" height="15" | &nbsp;
|style="font-weight:bold" width="65" | Low MW A
|style="font-weight:bold" width="65" | Low MW B
|style="font-weight:bold" width="65" | Low MW C
|style="font-weight:bold" width="65" | Low MW D
|style="font-weight:bold" width="65" | HIgh MW A
|style="font-weight:bold" width="65" | HIgh MW B
|style="font-weight:bold" width="65" | HIgh MW C
|style="font-weight:bold" width="65" | HIgh MW D
|- style="font-size:12pt" align="center"
|style="font-weight:bold" height="15" | ng/uL
| align="center" | 14
| align="center" | 8.1
| align="center" | 21.5
| align="center" | 23.6
| align="center" | 23.6
| align="center" | 0.6
| align="center" | 16.3
| align="center" | 24.8
|- style="font-size:12pt" align="center"
|style="font-weight:bold" height="15" | ug in 20 uL
| align="center" | 0.3
| align="center" | 0.2
| align="center" | 0.4
| align="center" | 0.5
| align="center" | 0.5
| align="center" | 0.0
| align="center" | 0.3
| align="center" | 0.5
|- style="font-size:12pt" align="center"
|style="font-weight:bold" height="15" | ug total
| align="center" | &nbsp;
| align="center" | &nbsp;
| align="center" | &nbsp;
| align="center" | 1.3
| align="center" | &nbsp;
| align="center" | &nbsp;
| align="center" | &nbsp;
| align="center" | 1.3
|}


'''Gel Image'''
'''Gel Image'''
{{GelLanes|Lane3=Low Mass Ladder|Lane4=High MW|Lane5=High MW|Lane6=High MW|Lane7=High MW|Lane8=Low MW|Lane9=Low MW|Lane10=Low MW|Lane12=Low MW}}


[[Image:2014-02-17-Cot1-HiLoMW.jpg|500px]]
[[Image:2014-02-17-Cot1-HiLoMW.jpg|500px]]
[[Category:HRCP]] [[Category:20140208]]

Latest revision as of 18:23, 2 July 2014

Multi-Color FISH (Started 02/08/2014)[edit]

Back to Calendar

Cot1 Labeling (Started 2/14/2014)[edit]

  1. Measure concentration of all samples in nanodrop
  2. Run on TBE gel to ensure correct size distribution
  3. Pool all high MW samples and all low MW samples

Results[edit]

Nanodrop Results

  Low MW A Low MW B Low MW C Low MW D HIgh MW A HIgh MW B HIgh MW C HIgh MW D
ng/uL 14 8.1 21.5 23.6 23.6 0.6 16.3 24.8
ug in 20 uL 0.3 0.2 0.4 0.5 0.5 0.0 0.3 0.5
ug total       1.3       1.3

Gel Image


Lane 1 2 3 4 5 6 7 8 9 10 11 12
Sample Low Mass Ladder High MW High MW High MW High MW Low MW Low MW Low MW Low MW

File:2014-02-17-Cot1-HiLoMW.jpg