Daniel:Notebook/HiResChrPaint/2014-2-22: Difference between revisions

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Had to put this on hold.  See [[Daniel:Notebook/HiResChrPaint/2014-2-23|tomorrow]]
Had to put this on hold.  See [[Daniel:Notebook/HiResChrPaint/2014-2-23|tomorrow]]
[[Category:HRCP]] [[Category:20140208]] [[Category:Hybridization]]

Latest revision as of 18:26, 2 July 2014

Multi-Color FISH (Started 02/08/2014)[edit]

Back to Calendar

Hybridization[edit]

Sample Matrix


Day 1

  1. Allow stored samples to warm to room temperature
  2. Incubate in 2X SSCT +50% (v/v) formamide for 2.5 minutes at 92 C in pre-warmed media
  3. Incubate in 2X SSCT + 50% formamide at 60C for 20 minutes
  4. Remove samples and allow to cool to room temperature
  5. Add 25 uL hybridization cocktail to center
  6. Add 22x22 #1.5 coverslip; seal with rubber cement
  7. Allow the rubber cement to air-dry for 5 minutes at room temperature
  8. Denature for 2.5 minutes at 92C; keep humid
  9. Transfer slides to a humidified chamber and hybridize overnight at 37C or 42C

Had to put this on hold. See tomorrow