Daniel:Notebook/HiResChrPaint/2014-2-24: Difference between revisions
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>Djacobse (Created page with "=Multi-Color FISH (Started 02/08/2014)= Back to Calendar ==Hybridization ([[Daniel:Notebook/HiRe...") |
>Djacobse No edit summary |
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''Day 2'' | ''Day 2'' | ||
#The next day, remove coverslip carefully and wash in pre-warmed 2X SSCT at 60C for 15 minutes | |||
#Incubate in 2X SSCT at room temperature for 10 minutes | |||
#Incubate in 0.2X SSC at room temperature for 10 minutes | |||
#Dry with kimwipes; do not let the surface containing the cells touch the kimwipes | |||
#Add 15 uL DRAQ5 (diluted 1:1000) to center of dish | |||
#Cover with 22x22 #1.5 coverslip; seal with nail polish | |||
##Allow at least 30 minutes for nail polish to dry | |||
##Slides are quite stable for several months at 4C | |||
#Waited 2 hours before going to microscope room | |||
==Microscopy== | |||
Imaged samples B,C, and D, but didn't have time for sample A (V6S3-546 only). See [[Daniel:Notebook/HiResChrPaint/2014-2-25|tomorrow]] for continued microscopy results. | |||
[[Category:HRCP]] [[Category:20140208]] [[Category:Hybridization]] |
Latest revision as of 18:27, 2 July 2014
Multi-Color FISH (Started 02/08/2014)[edit]
Hybridization (Started 02/23/2014)[edit]
Day 2
- The next day, remove coverslip carefully and wash in pre-warmed 2X SSCT at 60C for 15 minutes
- Incubate in 2X SSCT at room temperature for 10 minutes
- Incubate in 0.2X SSC at room temperature for 10 minutes
- Dry with kimwipes; do not let the surface containing the cells touch the kimwipes
- Add 15 uL DRAQ5 (diluted 1:1000) to center of dish
- Cover with 22x22 #1.5 coverslip; seal with nail polish
- Allow at least 30 minutes for nail polish to dry
- Slides are quite stable for several months at 4C
- Waited 2 hours before going to microscope room
Microscopy[edit]
Imaged samples B,C, and D, but didn't have time for sample A (V6S3-546 only). See tomorrow for continued microscopy results.