Song Chen:Daily/NOTES/2014-2-22: Difference between revisions

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>Chensong
(Created page with "===Cell density and DE differentiation=== Setting: Expose 1209 ms; Gain 5; Sharp 20; CERC epi-fluorescent microscope. {|{{table}} |<b>1x seeding density</b> |<b>3x seeding den...")
 
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===Cell density and DE differentiation===
===Cell density and DE differentiation===
Setting: Expose 1209 ms; Gain 5; Sharp 20; CERC epi-fluorescent microscope.
Setting: Expose 1000 ms, PFBH 406, 10x obj,
<font color=red>SOX17</font><b>/</b><font color=blue>DAPI</font>
{|{{table}}
{|{{table}}
|<b>1x seeding density</b>
|<b>1x seeding density</b>
Line 6: Line 7:
|<b>10x seeding density</b>
|<b>10x seeding density</b>
|-
|-
|[[File:022214_H9DE_1x_SOX17.jpg|300px]]
|[[File:022214_H9DE_1x_SOX17.png|300px]]
|[[File:022214_H9DE_3x_SOX17.jpg|300px]]
|[[File:022214_H9DE_3x_SOX17.png|300px]]
|[[File:022214_H9DE_10x_SOX17.jpg|300px]]
|[[File:022214_H9DE_10x_SOX17.png|300px]]
|-
|-
|[[File:022214_H9DE_1x_DAPI.jpg|300px]]
|[[File:022214_H9DE_1x_DAPI.png|300px]]
|[[File:022214_H9DE_3x_DAPI.jpg|300px]]
|[[File:022214_H9DE_3x_DAPI.png|300px]]
|[[File:022214_H9DE_10x_DAPI.jpg|300px]]
|[[File:022214_H9DE_10x_DAPI.png|300px]]
|}
|}


DE differentiation condition: D1 ActA, D2-4 ActA+0.2%FBS
DE differentiation condition: D1 ActA100, D2-4 ActA100+0.2%FBS
 
1x seeding density: D6-D7 hES passage at 1:12-1:15 ratio
1x seeding density: D6-D7 hES passage at 1:12-1:15 ratio
High seeding density results in high efficient DE differentiation
High seeding density results in high efficient DE differentiation


<br/><b>[[Song:Daily_Notebook|Return]]</b>
<br/><b>[[Song:Daily_Notebook|Return]]</b>

Latest revision as of 02:37, 25 February 2014

Cell density and DE differentiation[edit]

Setting: Expose 1000 ms, PFBH 406, 10x obj, SOX17/DAPI

1x seeding density 3x seeding density 10x seeding density
File:022214 H9DE 1x SOX17.png File:022214 H9DE 3x SOX17.png File:022214 H9DE 10x SOX17.png
File:022214 H9DE 1x DAPI.png File:022214 H9DE 3x DAPI.png File:022214 H9DE 10x DAPI.png

DE differentiation condition: D1 ActA100, D2-4 ActA100+0.2%FBS

1x seeding density: D6-D7 hES passage at 1:12-1:15 ratio

High seeding density results in high efficient DE differentiation


Return