Song Chen:Daily/NOTES/2014-2-21: Difference between revisions
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>Chensong (Created page with "===Hepatic commitment from high-density DE differentiation=== Setting: Expose 1000 ms, PFBH 406, 10x obj, <font color=green>AFP</font><b>/</b><font color=red>HNF4A</font>; <fo...") |
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<b>Hepatic commitment from high-density DE differentiation</b> | |||
Setting: Expose 1000 ms, PFBH 406, 10x obj, | Setting: Expose 1000 ms, PFBH 406, 10x obj, | ||
{|{{table}} | {|{{table}} | ||
|<b><font color=green>AFP</font | |<b><font color=green>AFP</font>/<font color=red>HNF4A</font></b> | ||
|<b><font color=green>AFP</font | |<b><font color=green>AFP</font>/<font color=red>HNF6</font></b> | ||
|<b><font color=green>AFP</font | |<b><font color=green>AFP</font>/<font color=red>PROX1</font></b> | ||
|- | |- | ||
|[[File:022114_H9DE_AFP.png|300px]] | |[[File:022114_H9DE_AFP.png|300px]] | ||
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Successful hepatic differentiation of high-density DE differentiated cells | Successful hepatic differentiation of high-density DE differentiated cells | ||
<b>hES change medium</b>: | |||
#H9 p60 2*6 cm hES medium | |||
#PGP1 iPS p30 1*6cm hES medium | |||
#H9 p60 2*6 cm NHSM | |||
<b>Hepatic differentiation from PGP1 iPS</b>:<br/> | |||
:16 well*48 well plate in DMEM/ITS | |||
<br/><b>[[Song:Daily_Notebook|Return]]</b> | <br/><b>[[Song:Daily_Notebook|Return]]</b> |
Latest revision as of 23:09, 28 February 2014
Hepatic commitment from high-density DE differentiation
Setting: Expose 1000 ms, PFBH 406, 10x obj,
AFP/HNF4A | AFP/HNF6 | AFP/PROX1 |
File:022114 H9DE AFP.png | File:022114 H9DE AFP.2.png | File:022114 H9DE AFP.3.png |
File:022114 H9DE HNF4A.png | File:022114 H9DE HNF6.png | File:022114 H9DE PROX1.png |
DE differentiation condition: D1 ActA100, D2-4 ActA100+0.2%FBS
High seeding density: D6-D7 hES passage at 1:1.2-1:1.5 ratio
Successful hepatic differentiation of high-density DE differentiated cells
hES change medium:
- H9 p60 2*6 cm hES medium
- PGP1 iPS p30 1*6cm hES medium
- H9 p60 2*6 cm NHSM
Hepatic differentiation from PGP1 iPS:
- 16 well*48 well plate in DMEM/ITS