Song Chen:Daily/NOTES/2014-2-21: Difference between revisions

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===Hepatic commitment from high-density DE differentiation===
<b>Hepatic commitment from high-density DE differentiation</b>
 
Setting: Expose 1000 ms, PFBH 406, 10x obj,
Setting: Expose 1000 ms, PFBH 406, 10x obj,
<font color=green>AFP</font><b>/</b><font color=red>HNF4A</font>;
<font color=green>AFP</font><b>/</b><font color=red>HNF6</font>;
<font color=green>AFP</font><b>/</b><font color=red>PROX1</font>;


{|{{table}}
{|{{table}}
|<b><font color=green>AFP</font><b>/</b><font color=red>HNF4A</font></b>
|<b><font color=green>AFP</font>/<font color=red>HNF4A</font></b>
|<b><font color=green>AFP</font><b>/</b><font color=red>HNF6</font></b>
|<b><font color=green>AFP</font>/<font color=red>HNF6</font></b>
|<b><font color=green>AFP</font><b>/</b><font color=red>PROX1</font></b>
|<b><font color=green>AFP</font>/<font color=red>PROX1</font></b>
|-
|-
|[[File:022114_H9DE_AFP.png|300px]]
|[[File:022114_H9DE_AFP.png|300px]]
Line 24: Line 22:


Successful hepatic differentiation of high-density DE differentiated cells
Successful hepatic differentiation of high-density DE differentiated cells
<b>hES change medium</b>:
#H9 p60 2*6 cm hES medium
#PGP1 iPS p30 1*6cm hES medium
#H9 p60 2*6 cm NHSM
<b>Hepatic differentiation from PGP1 iPS</b>:<br/>
:16 well*48 well plate in DMEM/ITS


<br/><b>[[Song:Daily_Notebook|Return]]</b>
<br/><b>[[Song:Daily_Notebook|Return]]</b>

Latest revision as of 23:09, 28 February 2014

Hepatic commitment from high-density DE differentiation

Setting: Expose 1000 ms, PFBH 406, 10x obj,

AFP/HNF4A AFP/HNF6 AFP/PROX1
File:022114 H9DE AFP.png File:022114 H9DE AFP.2.png File:022114 H9DE AFP.3.png
File:022114 H9DE HNF4A.png File:022114 H9DE HNF6.png File:022114 H9DE PROX1.png

DE differentiation condition: D1 ActA100, D2-4 ActA100+0.2%FBS

High seeding density: D6-D7 hES passage at 1:1.2-1:1.5 ratio

Successful hepatic differentiation of high-density DE differentiated cells


hES change medium:

  1. H9 p60 2*6 cm hES medium
  2. PGP1 iPS p30 1*6cm hES medium
  3. H9 p60 2*6 cm NHSM


Hepatic differentiation from PGP1 iPS:

16 well*48 well plate in DMEM/ITS


Return