Hosuk:LabNotes/2014-3-4: Difference between revisions

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===PGP1F cultured with noEGF media : Count Rolonies in 3D===
===PGP1F cultured with noEGF media : Count Rolonies in 3D===
*Data imgaes : [[Hosuk:LabNotes/2014-3-3|03/03]]
*Data imgaes : [[Hosuk:LabNotes/2014-3-3|03/03]]
*'''Search range : +/- 3 pixels''' which is too wide! I re-scan with +/- 1 pixel (see [[Hosuk:LabNotes/2014-3-5|03/05]])




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*ACTB
*ACTB
**Total count = 2164(~ 2.69x of MIP), back multi = 5
**Total count = 2164 (~ 2.69x of MIP)
**Counted with MIP = 803
**Counted with MIP = 803




*RAB7A
*RAB7A
**Total count = 5005(~1.74x of MIP), back multi = 5
**Total count = 5005 (~1.74x of MIP)
**Counted with MIP = 2865
**Counted with MIP = 2865




*MALAT1
*MALAT1
**Total count = 960(~ 8.89x of MIP), back multi = 5
**Total count = 960 (~ 8.89x of MIP)
**Counted with MIP = 108
**Counted with MIP = 108

Latest revision as of 18:15, 6 March 2014

PGP1F cultured with noEGF media : Count Rolonies in 3D[edit]

  • Data imgaes : 03/03
  • Search range : +/- 3 pixels which is too wide! I re-scan with +/- 1 pixel (see 03/05)


  • 1st Rolony data
    • Total count = 38303 (~ 1.51x of MIP)
    • Counted with MIP = 25295


  • ACTB
    • Total count = 2164 (~ 2.69x of MIP)
    • Counted with MIP = 803


  • RAB7A
    • Total count = 5005 (~1.74x of MIP)
    • Counted with MIP = 2865


  • MALAT1
    • Total count = 960 (~ 8.89x of MIP)
    • Counted with MIP = 108