Hosuk:LabNotes/2014-3-4: Difference between revisions
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===PGP1F cultured with noEGF media : Count Rolonies in 3D=== | ===PGP1F cultured with noEGF media : Count Rolonies in 3D=== | ||
*Data imgaes : [[Hosuk:LabNotes/2014-3-3|03/03]] | *Data imgaes : [[Hosuk:LabNotes/2014-3-3|03/03]] | ||
*'''Search range : +/- 3 pixels''' which is too wide! I re-scan with +/- 1 pixel (see [[Hosuk:LabNotes/2014-3-5|03/05]]) | |||
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*ACTB | *ACTB | ||
**Total count = 2164(~ 2.69x of MIP) | **Total count = 2164 (~ 2.69x of MIP) | ||
**Counted with MIP = 803 | **Counted with MIP = 803 | ||
*RAB7A | *RAB7A | ||
**Total count = 5005(~1.74x of MIP) | **Total count = 5005 (~1.74x of MIP) | ||
**Counted with MIP = 2865 | **Counted with MIP = 2865 | ||
*MALAT1 | *MALAT1 | ||
**Total count = 960(~ 8.89x of MIP) | **Total count = 960 (~ 8.89x of MIP) | ||
**Counted with MIP = 108 | **Counted with MIP = 108 |
Latest revision as of 18:15, 6 March 2014
PGP1F cultured with noEGF media : Count Rolonies in 3D[edit]
- Data imgaes : 03/03
- Search range : +/- 3 pixels which is too wide! I re-scan with +/- 1 pixel (see 03/05)
- 1st Rolony data
- Total count = 38303 (~ 1.51x of MIP)
- Counted with MIP = 25295
- ACTB
- Total count = 2164 (~ 2.69x of MIP)
- Counted with MIP = 803
- RAB7A
- Total count = 5005 (~1.74x of MIP)
- Counted with MIP = 2865
- MALAT1
- Total count = 960 (~ 8.89x of MIP)
- Counted with MIP = 108