EricChu:LabNotesMDA/2014-3-5: Difference between revisions

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>Ericchu
(Created page with "==PGP1#22 Tagmentation with Pol1/DNA precipitation== * 1ul of each sample was prepped with Pol1. They were all saved at 4°C. ==PGP1#22 Tagmentation without Pol1== * Started ...")
 
>Ericchu
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Line 4: Line 4:
==PGP1#22 Tagmentation without Pol1==
==PGP1#22 Tagmentation without Pol1==
* Started the sample tagmentation with transposases.
* Started the sample tagmentation with transposases.
* Nextera indexes 49-72 were used for sample 1-24. Index 13 was assigned to sample 25 (ring) and index 14 to neg control.
* Here are Tag PCR1 and PCR2.
* Here are Tag PCR1 and PCR2.
[[File:03052014TagPCR1-NoPol.jpg]]
[[File:03052014TagPCR1-NoPol.jpg]]
[[File:03052014TagPCR2-NoPol.jpg]]
[[File:03052014TagPCR2-NoPol.jpg]]

Latest revision as of 21:03, 17 March 2014

PGP1#22 Tagmentation with Pol1/DNA precipitation[edit]

  • 1ul of each sample was prepped with Pol1. They were all saved at 4°C.

PGP1#22 Tagmentation without Pol1[edit]

  • Started the sample tagmentation with transposases.
  • Nextera indexes 49-72 were used for sample 1-24. Index 13 was assigned to sample 25 (ring) and index 14 to neg control.
  • Here are Tag PCR1 and PCR2.

File:03052014TagPCR1-NoPol.jpg File:03052014TagPCR2-NoPol.jpg