EricChu:LabNotesMDA/2014-3-5: Difference between revisions
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>Ericchu (Created page with "==PGP1#22 Tagmentation with Pol1/DNA precipitation== * 1ul of each sample was prepped with Pol1. They were all saved at 4°C. ==PGP1#22 Tagmentation without Pol1== * Started ...") |
>Ericchu No edit summary |
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==PGP1#22 Tagmentation without Pol1== | ==PGP1#22 Tagmentation without Pol1== | ||
* Started the sample tagmentation with transposases. | * Started the sample tagmentation with transposases. | ||
* Nextera indexes 49-72 were used for sample 1-24. Index 13 was assigned to sample 25 (ring) and index 14 to neg control. | |||
* Here are Tag PCR1 and PCR2. | * Here are Tag PCR1 and PCR2. | ||
[[File:03052014TagPCR1-NoPol.jpg]] | [[File:03052014TagPCR1-NoPol.jpg]] | ||
[[File:03052014TagPCR2-NoPol.jpg]] | [[File:03052014TagPCR2-NoPol.jpg]] |
Latest revision as of 21:03, 17 March 2014
PGP1#22 Tagmentation with Pol1/DNA precipitation[edit]
- 1ul of each sample was prepped with Pol1. They were all saved at 4°C.
PGP1#22 Tagmentation without Pol1[edit]
- Started the sample tagmentation with transposases.
- Nextera indexes 49-72 were used for sample 1-24. Index 13 was assigned to sample 25 (ring) and index 14 to neg control.
- Here are Tag PCR1 and PCR2.
File:03052014TagPCR1-NoPol.jpg File:03052014TagPCR2-NoPol.jpg