EricChu:LabNotesMDA/2014-3-7: Difference between revisions

From ZhangLabWiki
Jump to navigation Jump to search
>Ericchu
No edit summary
>Ericchu
No edit summary
 
(3 intermediate revisions by the same user not shown)
Line 1: Line 1:
==PGP1#22 Tagmentation with Pol1==
* Finished Tag PCR1 and PCR2.
* Finished qPCR of Alu and Mito
[[File:03062014TagPCR1withPol1.jpg]]
[[File:03062014TagPCR2withPol1.jpg]]
* Left to Right: sample 1-10
[[File:03062014TagGel1.jpg]]
* Left to right: sample 11-20
[[File:03062014TagGel2.jpg]]
* left to right: sample 21-25 + neg control
[[File:03062014TagGel3.jpg]]
[[File:03062014TagwithPol1Alu.jpg]]
[[File:03062014TagwithPol1Mito.jpg]]
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Chamber'''
| align="center" style="background:#f0f0f0;"|'''Alu'''
| align="center" style="background:#f0f0f0;"|'''Mito'''
| align="center" style="background:#f0f0f0;"|'''Mito/Alu'''
|-
| 1||5,327.00||1,201.00||0.23
|-
| 2||5,559.00||1,086.00||0.20
|-
| 3||5,129.00||1,554.00||0.30
|-
| 4||6,633.00||300.40||0.05
|-
| 5||5,784.00||932.90||0.16
|-
| 6||5,997.00||31,670.00||5.28
|-
| 7||6,684.00||3,826.00||0.57
|-
| 8||10,430.00||562.70||0.05
|-
| 9||17,210.00||64,750.00||3.76
|-
| 10||5,708.00||4,642.00||0.81
|-
| 11||15,520.00||29,400.00||1.89
|-
| 12||10,300.00||5,134.00||0.50
|-
| 13||11,640.00||25,770.00||2.21
|-
| 14||15,230.00||18,150.00||1.19
|-
| 15||12,500.00||9,584.00||0.77
|-
| 16||15,700.00||24,560.00||1.56
|-
| 17||27,860.00||40,710.00||1.46
|-
| 18||21,430.00||43,310.00||2.02
|-
| 19||12,150.00||181,100.00||14.91
|-
| 20||22,570.00||87,760.00||3.89
|-
| 21||12,540.00||22,580.00||1.80
|-
| 22||11,220.00||38,830.00||3.46
|-
| 23||10,140.00||44,380.00||4.38
|-
| 24||6,802.00||5,077.00||0.75
|-
| 25||7,127.00||97.81||0.01
|-
| 26||N/A||27.95||#VALUE!
|-
|
|}
* Samples were pooled into 3 libraries according to the Alu reading
* EC-PGP1#22 Tag Lo Mar7: Index 49-55, 58
* EC-PGP1#22 Tag M Mar7: Index 56, 57, 59-64, 67, 69-71
* Ec-PGP1#22 Tag Hi Mar7: Index 13, 65, 66, 68, 72
==Library Verification==
[[File:03072014PGP1.jpg]]
[[File:03072014PGP1.jpg]]

Latest revision as of 21:36, 17 March 2014

PGP1#22 Tagmentation with Pol1[edit]

  • Finished Tag PCR1 and PCR2.
  • Finished qPCR of Alu and Mito

File:03062014TagPCR1withPol1.jpg File:03062014TagPCR2withPol1.jpg

  • Left to Right: sample 1-10

File:03062014TagGel1.jpg

  • Left to right: sample 11-20

File:03062014TagGel2.jpg

  • left to right: sample 21-25 + neg control

File:03062014TagGel3.jpg File:03062014TagwithPol1Alu.jpg File:03062014TagwithPol1Mito.jpg

Chamber Alu Mito Mito/Alu
1 5,327.00 1,201.00 0.23
2 5,559.00 1,086.00 0.20
3 5,129.00 1,554.00 0.30
4 6,633.00 300.40 0.05
5 5,784.00 932.90 0.16
6 5,997.00 31,670.00 5.28
7 6,684.00 3,826.00 0.57
8 10,430.00 562.70 0.05
9 17,210.00 64,750.00 3.76
10 5,708.00 4,642.00 0.81
11 15,520.00 29,400.00 1.89
12 10,300.00 5,134.00 0.50
13 11,640.00 25,770.00 2.21
14 15,230.00 18,150.00 1.19
15 12,500.00 9,584.00 0.77
16 15,700.00 24,560.00 1.56
17 27,860.00 40,710.00 1.46
18 21,430.00 43,310.00 2.02
19 12,150.00 181,100.00 14.91
20 22,570.00 87,760.00 3.89
21 12,540.00 22,580.00 1.80
22 11,220.00 38,830.00 3.46
23 10,140.00 44,380.00 4.38
24 6,802.00 5,077.00 0.75
25 7,127.00 97.81 0.01
26 N/A 27.95 #VALUE!
  • Samples were pooled into 3 libraries according to the Alu reading
  • EC-PGP1#22 Tag Lo Mar7: Index 49-55, 58
  • EC-PGP1#22 Tag M Mar7: Index 56, 57, 59-64, 67, 69-71
  • Ec-PGP1#22 Tag Hi Mar7: Index 13, 65, 66, 68, 72

Library Verification[edit]

File:03072014PGP1.jpg