EricChu:LabNotesMDA/2014-3-7: Difference between revisions
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[[File:03062014TagPCR1withPol1.jpg]] | [[File:03062014TagPCR1withPol1.jpg]] | ||
[[File:03062014TagPCR2withPol1.jpg]] | [[File:03062014TagPCR2withPol1.jpg]] | ||
* Left to Right: sample 1-10 | |||
[[File:03062014TagGel1.jpg]] | |||
* Left to right: sample 11-20 | |||
[[File:03062014TagGel2.jpg]] | |||
* left to right: sample 21-25 + neg control | |||
[[File:03062014TagGel3.jpg]] | |||
[[File:03062014TagwithPol1Alu.jpg]] | [[File:03062014TagwithPol1Alu.jpg]] | ||
[[File:03062014TagwithPol1Mito.jpg]] | [[File:03062014TagwithPol1Mito.jpg]] | ||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Chamber''' | |||
| align="center" style="background:#f0f0f0;"|'''Alu''' | |||
| align="center" style="background:#f0f0f0;"|'''Mito''' | |||
| align="center" style="background:#f0f0f0;"|'''Mito/Alu''' | |||
|- | |||
| 1||5,327.00||1,201.00||0.23 | |||
|- | |||
| 2||5,559.00||1,086.00||0.20 | |||
|- | |||
| 3||5,129.00||1,554.00||0.30 | |||
|- | |||
| 4||6,633.00||300.40||0.05 | |||
|- | |||
| 5||5,784.00||932.90||0.16 | |||
|- | |||
| 6||5,997.00||31,670.00||5.28 | |||
|- | |||
| 7||6,684.00||3,826.00||0.57 | |||
|- | |||
| 8||10,430.00||562.70||0.05 | |||
|- | |||
| 9||17,210.00||64,750.00||3.76 | |||
|- | |||
| 10||5,708.00||4,642.00||0.81 | |||
|- | |||
| 11||15,520.00||29,400.00||1.89 | |||
|- | |||
| 12||10,300.00||5,134.00||0.50 | |||
|- | |||
| 13||11,640.00||25,770.00||2.21 | |||
|- | |||
| 14||15,230.00||18,150.00||1.19 | |||
|- | |||
| 15||12,500.00||9,584.00||0.77 | |||
|- | |||
| 16||15,700.00||24,560.00||1.56 | |||
|- | |||
| 17||27,860.00||40,710.00||1.46 | |||
|- | |||
| 18||21,430.00||43,310.00||2.02 | |||
|- | |||
| 19||12,150.00||181,100.00||14.91 | |||
|- | |||
| 20||22,570.00||87,760.00||3.89 | |||
|- | |||
| 21||12,540.00||22,580.00||1.80 | |||
|- | |||
| 22||11,220.00||38,830.00||3.46 | |||
|- | |||
| 23||10,140.00||44,380.00||4.38 | |||
|- | |||
| 24||6,802.00||5,077.00||0.75 | |||
|- | |||
| 25||7,127.00||97.81||0.01 | |||
|- | |||
| 26||N/A||27.95||#VALUE! | |||
|- | |||
| | |||
|} | |||
* Samples were pooled into 3 libraries according to the Alu reading | |||
* EC-PGP1#22 Tag Lo Mar7: Index 49-55, 58 | |||
* EC-PGP1#22 Tag M Mar7: Index 56, 57, 59-64, 67, 69-71 | |||
* Ec-PGP1#22 Tag Hi Mar7: Index 13, 65, 66, 68, 72 | |||
==Library Verification== | ==Library Verification== | ||
[[File:03072014PGP1.jpg]] | [[File:03072014PGP1.jpg]] |
Latest revision as of 21:36, 17 March 2014
PGP1#22 Tagmentation with Pol1[edit]
- Finished Tag PCR1 and PCR2.
- Finished qPCR of Alu and Mito
File:03062014TagPCR1withPol1.jpg File:03062014TagPCR2withPol1.jpg
- Left to Right: sample 1-10
- Left to right: sample 11-20
- left to right: sample 21-25 + neg control
File:03062014TagGel3.jpg File:03062014TagwithPol1Alu.jpg File:03062014TagwithPol1Mito.jpg
Chamber | Alu | Mito | Mito/Alu |
1 | 5,327.00 | 1,201.00 | 0.23 |
2 | 5,559.00 | 1,086.00 | 0.20 |
3 | 5,129.00 | 1,554.00 | 0.30 |
4 | 6,633.00 | 300.40 | 0.05 |
5 | 5,784.00 | 932.90 | 0.16 |
6 | 5,997.00 | 31,670.00 | 5.28 |
7 | 6,684.00 | 3,826.00 | 0.57 |
8 | 10,430.00 | 562.70 | 0.05 |
9 | 17,210.00 | 64,750.00 | 3.76 |
10 | 5,708.00 | 4,642.00 | 0.81 |
11 | 15,520.00 | 29,400.00 | 1.89 |
12 | 10,300.00 | 5,134.00 | 0.50 |
13 | 11,640.00 | 25,770.00 | 2.21 |
14 | 15,230.00 | 18,150.00 | 1.19 |
15 | 12,500.00 | 9,584.00 | 0.77 |
16 | 15,700.00 | 24,560.00 | 1.56 |
17 | 27,860.00 | 40,710.00 | 1.46 |
18 | 21,430.00 | 43,310.00 | 2.02 |
19 | 12,150.00 | 181,100.00 | 14.91 |
20 | 22,570.00 | 87,760.00 | 3.89 |
21 | 12,540.00 | 22,580.00 | 1.80 |
22 | 11,220.00 | 38,830.00 | 3.46 |
23 | 10,140.00 | 44,380.00 | 4.38 |
24 | 6,802.00 | 5,077.00 | 0.75 |
25 | 7,127.00 | 97.81 | 0.01 |
26 | N/A | 27.95 | #VALUE! |
- Samples were pooled into 3 libraries according to the Alu reading
- EC-PGP1#22 Tag Lo Mar7: Index 49-55, 58
- EC-PGP1#22 Tag M Mar7: Index 56, 57, 59-64, 67, 69-71
- Ec-PGP1#22 Tag Hi Mar7: Index 13, 65, 66, 68, 72