Hosuk:LabNotes/2014-7-9: Difference between revisions

From ZhangLabWiki
Jump to navigation Jump to search
>Hosuki78
No edit summary
>Hosuki78
No edit summary
 
Line 10: Line 10:
**Compare to fixing with 10% Formalin at 25C : [[Hosuk:LabNotes/2014-7-4|LabNote]]
**Compare to fixing with 10% Formalin at 25C : [[Hosuk:LabNotes/2014-7-4|LabNote]]


*Counts
**PGP1F, 20x obj = 22311
**PGP1F, 20x obj = 7064
**NCIH1975, 20x obj = 18134
**NCIH1975, 63x obj = 5493





Latest revision as of 21:23, 11 July 2014

NCIH1975, PGP1F with T48 RT Primer[edit]

  • Fix NCIH1975 and PGP1F with 4% Formalin at 37C
  • S1 : T48 RT primer, S2 : rHexamer

Result[edit]

  • Rolony with T48 : 30~50% less than rolony with rHex
  • Fixing with 4% Formalin at 37C seems better in terms of even distribution of rolonies, which meaning less localized in nuclear
    • Compare to fixing with 10% Formalin at 25C : LabNote


  • Counts
    • PGP1F, 20x obj = 22311
    • PGP1F, 20x obj = 7064
    • NCIH1975, 20x obj = 18134
    • NCIH1975, 63x obj = 5493


File:Images PGP1F NCIH1975 20140709 rHex 20x 63x.png


Tissue 11th[edit]

  • Tissue 11th: 10um thick, random hexamer, -a-dUTP

Result[edit]

  • Result : Tissue 11th: not as many as expected, similar to 9th result


File:Images Tissue 11th 20140709 rHex 20x Pos1 Pos2.png