Noi/NOTES/2014-8-13: Difference between revisions
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>Noi (Created page with "* noi:DMR220k_LabNotes * 2014-08-13: Received MOMOD V4 Oligo pool from CustomAraay === Oligo info. === * Name: 90K_ologos_04Aug2014 * Conc. 20.53ng/ul, vol. 80ul, amount 1...") |
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* [[noi:DMR220k_LabNotes]] | = Expansion PCR of new probe set from CustomArrays (90K_ologos_04Aug2014) = | ||
* 2014-08-13: Received MOMOD V4 Oligo pool from CustomAraay | * [[noi:DMR220k_LabNotes| '''Link to calendar''']] | ||
* 2014-08-13: Received MOMOD V4 Oligo (Dr. Zhang recently called V5) pool from CustomAraay | |||
=== Oligo info. === | === Oligo info. === | ||
* Name: 90K_ologos_04Aug2014 | * Name: 90K_ologos_04Aug2014 | ||
* Conc. 20.53ng/ul, | * Conc. 20.53ng/ul, | ||
* Volume 80ul in TE buffer. Total amount 1.64ug | |||
* Info from Dr. Zhang's note | * Info from Dr. Zhang's note | ||
Description # oligos Length Amp. Primers Adaptor Removal | Description # oligos Length Amp. Primers Adaptor Removal | ||
Line 11: | Line 13: | ||
Total: 89,107 | Total: 89,107 | ||
* will be working on 73K probe set | * I will be working on 73K probe set | ||
* Length 148 mer (MW = 148 * 330Da/bp = 48,840 Da or g/mole | |||
* Conc. 20.53ng/ul = 420.35nM (20.53ng/ul/ 48,840g/mole) | |||
== Expansion PCR == | |||
95C 30sec -> (95C 30sec -> 54C 45sec-> 72C 45sec) X 16-> 72C 3min -> 15C hold<br> | |||
:'''-There is something wrong with the oligo pool since I can not amplify all the three subsets of the probes, but it worked for positive control of the three subsets.''' | |||
:'''-We will get CustomArray to re-synthesize the probes''' |
Latest revision as of 17:02, 24 December 2014
Expansion PCR of new probe set from CustomArrays (90K_ologos_04Aug2014)[edit]
- Link to calendar
- 2014-08-13: Received MOMOD V4 Oligo (Dr. Zhang recently called V5) pool from CustomAraay
Oligo info.[edit]
- Name: 90K_ologos_04Aug2014
- Conc. 20.53ng/ul,
- Volume 80ul in TE buffer. Total amount 1.64ug
- Info from Dr. Zhang's note
Description # oligos Length Amp. Primers Adaptor Removal Set A: BSPP LMS cluster probes 73,574 148nt V6 USER/DpnII Set B: Selector LMS cluster 12,994 148nt V4 USER/DpnII Set C: Selector chr21 unique 2,539 148nt eMIP_CA_F/R Nt.AlwI/Nb.BrsDI Total: 89,107
- I will be working on 73K probe set
- Length 148 mer (MW = 148 * 330Da/bp = 48,840 Da or g/mole
- Conc. 20.53ng/ul = 420.35nM (20.53ng/ul/ 48,840g/mole)
Expansion PCR[edit]
95C 30sec -> (95C 30sec -> 54C 45sec-> 72C 45sec) X 16-> 72C 3min -> 15C hold
- -There is something wrong with the oligo pool since I can not amplify all the three subsets of the probes, but it worked for positive control of the three subsets.
- -We will get CustomArray to re-synthesize the probes