Daniel:Notebook/RNAFISH/2014-8-29: Difference between revisions

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>Djacobse
(Created page with "=Trial Run= Back to Calendar ==Tissue Section Preparation== Following Tissue FISH Protocol: #Fix tissue pieces ...")
 
>Djacobse
 
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Following [[Daniel:Protocols/TissueFISH|Tissue FISH Protocol]]:
Following [[Daniel:Protocols/TissueFISH|Tissue FISH Protocol]]:


#Fix tissue pieces of an appropriate size in freshly prepared 4% formaldehyde/PBS for 4–24 h
#Fix tissue pieces of an appropriate size in freshly prepared 4% formaldehyde/PBS overnight at 4 C (start time 2pm)
#Wash the tissue in PBS, 3 × 30 min. Tissue can be stored in PBS for few days at +4°C
#Continued [[Daniel:Notebook/RNAFISH/2014-8-30|tomorrow]]
 
[[Category:RNAFISH]] [[Category:20140829]]

Latest revision as of 17:11, 24 September 2014

Trial Run[edit]

Back to Calendar

Tissue Section Preparation[edit]

Following Tissue FISH Protocol:

  1. Fix tissue pieces of an appropriate size in freshly prepared 4% formaldehyde/PBS overnight at 4 C (start time 2pm)
  2. Continued tomorrow