Noi/NOTES/2014-8-25: Difference between revisions

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= Additional cfDNA from Illumina (Nautica cfDNA) =
= Additional cfDNA from Illumina (Nautica cfDNA) =
[[noi:DMR220k_LabNotes| '''Back to calendar''']]
* Dr. Zhang received 3 additional cfDNA from Illumina  
* Dr. Zhang received 3 additional cfDNA from Illumina  
* Sample info. from Rigo
* Sample info. from Rigo
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* I am not sure how they measure DNA concentration. I will repeat measuring concentration again.
* I am not sure how they measure DNA concentration. I will repeat measuring concentration again.
* To avoid losing too much DNA for re-quantification, I will add more ~7ul of EB buffer to bring the volume to 21ul and use 1ul for Qubit dsDNA quantification.
* To avoid losing too much DNA for re-quantification, I will add more ~7ul of EB buffer to bring the volume to 21ul and use 1ul for Qubit dsDNA quantification.
'''2014-08-30'''
==== Re-quantification of cfDNA by Qubit dsDNA HS assay ====
{| {{table}} class = wikitable
| align="center" style="width:140px;background:#f0f0f0;"|'''Sample'''
| align="center" style="width:100px;background:#f0f0f0;"|'''Conc. By Qubit (ng/ul)'''
| align="center" style="width:100px;background:#f0f0f0;"|'''Amount in 20ul (ng)'''
|-
| NORM1-02 P1||1.66||33.20
|-
| NORM1-03 P1||0.77||15.30
|-
| COLO2-03||1.11||22.20
|}
* I guess that the concentration of cfDNA from Rigo is by Nanodrop. Those numbers seemed to be over-estimated since the total amount by Qubit is much lower than that I got from Illumina.
* Continue to WGBS library prep by me: [[http://genome-tech.ucsd.edu/LabNotes/index.php/Noi/NOTES/2014-9-8]]

Latest revision as of 13:00, 24 December 2014

Additional cfDNA from Illumina (Nautica cfDNA)[edit]

Back to calendar

  • Dr. Zhang received 3 additional cfDNA from Illumina
  • Sample info. from Rigo
Sample Conc. (ng/ul) Volume (ul) Amount (ng)
NORM1-02 P1 5.25 15.00 78.70
NORM1-03 P1 3.25 15.00 48.70
COLO2-03 3.05 15.00 45.80
  • I am not sure how they measure DNA concentration. I will repeat measuring concentration again.
  • To avoid losing too much DNA for re-quantification, I will add more ~7ul of EB buffer to bring the volume to 21ul and use 1ul for Qubit dsDNA quantification.

2014-08-30

Re-quantification of cfDNA by Qubit dsDNA HS assay[edit]

Sample Conc. By Qubit (ng/ul) Amount in 20ul (ng)
NORM1-02 P1 1.66 33.20
NORM1-03 P1 0.77 15.30
COLO2-03 1.11 22.20
  • I guess that the concentration of cfDNA from Rigo is by Nanodrop. Those numbers seemed to be over-estimated since the total amount by Qubit is much lower than that I got from Illumina.
  • Continue to WGBS library prep by me: [[1]]