Noi/NOTES/2014-8-25: Difference between revisions
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= Additional cfDNA from Illumina (Nautica cfDNA) = | = Additional cfDNA from Illumina (Nautica cfDNA) = | ||
[[noi:DMR220k_LabNotes| '''Back to calendar''']] | |||
* Dr. Zhang received 3 additional cfDNA from Illumina | * Dr. Zhang received 3 additional cfDNA from Illumina | ||
* Sample info. from Rigo | * Sample info. from Rigo | ||
Line 16: | Line 17: | ||
* I am not sure how they measure DNA concentration. I will repeat measuring concentration again. | * I am not sure how they measure DNA concentration. I will repeat measuring concentration again. | ||
* To avoid losing too much DNA for re-quantification, I will add more ~7ul of EB buffer to bring the volume to 21ul and use 1ul for Qubit dsDNA quantification. | * To avoid losing too much DNA for re-quantification, I will add more ~7ul of EB buffer to bring the volume to 21ul and use 1ul for Qubit dsDNA quantification. | ||
'''2014-08-30''' | |||
==== Re-quantification of cfDNA by Qubit dsDNA HS assay ==== | |||
{| {{table}} class = wikitable | |||
| align="center" style="width:140px;background:#f0f0f0;"|'''Sample''' | |||
| align="center" style="width:100px;background:#f0f0f0;"|'''Conc. By Qubit (ng/ul)''' | |||
| align="center" style="width:100px;background:#f0f0f0;"|'''Amount in 20ul (ng)''' | |||
|- | |||
| NORM1-02 P1||1.66||33.20 | |||
|- | |||
| NORM1-03 P1||0.77||15.30 | |||
|- | |||
| COLO2-03||1.11||22.20 | |||
|} | |||
* I guess that the concentration of cfDNA from Rigo is by Nanodrop. Those numbers seemed to be over-estimated since the total amount by Qubit is much lower than that I got from Illumina. | |||
* Continue to WGBS library prep by me: [[http://genome-tech.ucsd.edu/LabNotes/index.php/Noi/NOTES/2014-9-8]] |
Latest revision as of 13:00, 24 December 2014
Additional cfDNA from Illumina (Nautica cfDNA)[edit]
- Dr. Zhang received 3 additional cfDNA from Illumina
- Sample info. from Rigo
Sample | Conc. (ng/ul) | Volume (ul) | Amount (ng) |
NORM1-02 P1 | 5.25 | 15.00 | 78.70 |
NORM1-03 P1 | 3.25 | 15.00 | 48.70 |
COLO2-03 | 3.05 | 15.00 | 45.80 |
- I am not sure how they measure DNA concentration. I will repeat measuring concentration again.
- To avoid losing too much DNA for re-quantification, I will add more ~7ul of EB buffer to bring the volume to 21ul and use 1ul for Qubit dsDNA quantification.
2014-08-30
Re-quantification of cfDNA by Qubit dsDNA HS assay[edit]
Sample | Conc. By Qubit (ng/ul) | Amount in 20ul (ng) |
NORM1-02 P1 | 1.66 | 33.20 |
NORM1-03 P1 | 0.77 | 15.30 |
COLO2-03 | 1.11 | 22.20 |
- I guess that the concentration of cfDNA from Rigo is by Nanodrop. Those numbers seemed to be over-estimated since the total amount by Qubit is much lower than that I got from Illumina.
- Continue to WGBS library prep by me: [[1]]