Hosuk:LabNotes/2014-9-5: Difference between revisions
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>Hosuki78 (Created page with "*LabNote ===PGP1F 2nd rolonyar20140414 – Decode again with 63x=== *63x, zoom=1, 2x2 tile (each tile index : s0~s3, --> converted to s1~s4 for analysis)...") |
>Hosuki78 No edit summary |
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====Merged | =====Merged and each Tile image of BF, 1st rolonies===== | ||
[[File:Merged_Tile_BF.png|800px]] | |||
[[File:Merged_Tile_1stRolony.png|800px]] | |||
=====Mapped table and intensity plot===== | |||
[[File:GeneMapTable_IntPlot_s1.png|600px]] | |||
[[File:GeneMapTable_IntPlot_s2.png|600px]] | |||
[[File:GeneMapTable_IntPlot_s3.png|600px]] | |||
[[File:GeneMapTable_IntPlot_s4.png|600px]] | |||
=====Mapped gene position (S2)===== | |||
[[File:OverlapMark_s2.png|600px]] | |||
=====Mapped gene count in all 4 tiles ===== | |||
[[File:GeneMapTable_All_AgiPPgDNACount2.png|700px]] | |||
=====Compare to gDNACount of Agi26k0gap Padlock probe ===== | |||
[[File:GeneMapTable_All_AgiPPgDNACount_Plot2.png|400px]] |
Latest revision as of 20:44, 7 September 2014
PGP1F 2nd rolonyar20140414 – Decode again with 63x[edit]
- 63x, zoom=1, 2x2 tile (each tile index : s0~s3, --> converted to s1~s4 for analysis)
- Z=0.49um, ~22 images
Result[edit]
- Image signal were too saturated… need to use less PMT gain… (this time it was 660, 4% laser)
Merged and each Tile image of BF, 1st rolonies[edit]
File:Merged Tile BF.png File:Merged Tile 1stRolony.png
Mapped table and intensity plot[edit]
File:GeneMapTable IntPlot s1.png File:GeneMapTable IntPlot s2.png File:GeneMapTable IntPlot s3.png File:GeneMapTable IntPlot s4.png
Mapped gene position (S2)[edit]
Mapped gene count in all 4 tiles[edit]
File:GeneMapTable All AgiPPgDNACount2.png