Hosuk:LabNotes/2014-9-5: Difference between revisions

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>Hosuki78
(Created page with "*LabNote ===PGP1F 2nd rolonyar20140414 – Decode again with 63x=== *63x, zoom=1, 2x2 tile (each tile index : s0~s3, --> converted to s1~s4 for analysis)...")
 
>Hosuki78
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====Merged, Tile, BF====
=====Merged and each Tile image of BF, 1st rolonies=====
*[[File:Merged_Tile_BF.png|600px]]
[[File:Merged_Tile_BF.png|800px]]
*[[File:Merged_Tile_1stRolony.png|600px]]
[[File:Merged_Tile_1stRolony.png|800px]]




*[[File:GeneMapTable_IntPlot_s1.png|600px]]
=====Mapped table and intensity plot=====
*[[File:GeneMapTable_IntPlot_s2.png|600px]]
[[File:GeneMapTable_IntPlot_s1.png|600px]]
*[[File:GeneMapTable_IntPlot_s3.png|600px]]
[[File:GeneMapTable_IntPlot_s2.png|600px]]
*[[File:GeneMapTable_IntPlot_s4.png|600px]]
[[File:GeneMapTable_IntPlot_s3.png|600px]]
[[File:GeneMapTable_IntPlot_s4.png|600px]]




*[[File:OverlapMark_s2.png|600px]]
=====Mapped gene position (S2)=====
[[File:OverlapMark_s2.png|600px]]




*[[File:GeneMapTable_All_AgiPPgDNACount.png|600px]]
=====Mapped gene count in all 4 tiles =====
*[[File:GeneMapTable_All_AgiPPgDNACount_Plot.png|600px]]
[[File:GeneMapTable_All_AgiPPgDNACount2.png|700px]]
 
 
=====Compare to gDNACount of Agi26k0gap Padlock probe =====
[[File:GeneMapTable_All_AgiPPgDNACount_Plot2.png|400px]]

Latest revision as of 20:44, 7 September 2014


PGP1F 2nd rolonyar20140414 – Decode again with 63x[edit]

  • 63x, zoom=1, 2x2 tile (each tile index : s0~s3, --> converted to s1~s4 for analysis)
  • Z=0.49um, ~22 images


Result[edit]

  • Image signal were too saturated… need to use less PMT gain… (this time it was 660, 4% laser)


Merged and each Tile image of BF, 1st rolonies[edit]

File:Merged Tile BF.png File:Merged Tile 1stRolony.png


Mapped table and intensity plot[edit]

File:GeneMapTable IntPlot s1.png File:GeneMapTable IntPlot s2.png File:GeneMapTable IntPlot s3.png File:GeneMapTable IntPlot s4.png


Mapped gene position (S2)[edit]

File:OverlapMark s2.png


Mapped gene count in all 4 tiles[edit]

File:GeneMapTable All AgiPPgDNACount2.png


Compare to gDNACount of Agi26k0gap Padlock probe[edit]

File:GeneMapTable All AgiPPgDNACount Plot2.png