Daniel:CellLines/U87MG: Difference between revisions

From ZhangLabWiki
Jump to navigation Jump to search
>Djacobse
>Djacobse
No edit summary
 
(One intermediate revision by the same user not shown)
Line 3: Line 3:
[[Daniel Jacobsen|Back to Main]]
[[Daniel Jacobsen|Back to Main]]


[http://www.sigmaaldrich.com/catalog/product/sigma/89081402?lang=en&region=US&gclid=CPrH4qzY6cECFQlgfgodcJcA3Q Info from Sigma Aldrich]
[http://www.atcc.org/products/all/HTB-14.aspx ATCC Website]


'''Cell Line Origin''':Human glioblastoma astrocytoma
[http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM587180 Gene Expression (NCBI)]


'''Cell Line Description''': Derived from a malignant glioma from a female patient by explant technique. It is reported to produce a malignant tumour consistent with glioblastoma in nude mice.
'''Morphology''': Epithelial Cell


'''Media''':EMEM (EBSS) +
'''Cell Line Description''': This is a hypodiploid human cell line with the modal chromosome number of 44 occurring in 48% of cells. The rate of higher ploidy was 5.9%., Twelve markers were common to all cells, including der(1)t(1;3) (p22;q21), der(16)t(1;16) (p22;p12), del(9) (p13) and nine others. The marker der(1) had two copies in most cells. There was only one copy of normal X. N1, N6 and N9 were not found. 
*2mM Glutamine
 
*1% Non Essential Amino Acids (NEAA)
'''Media''':EMEM (EBSS)
*1mM Sodium Pyruvate (NaP)  
*10% Fetal Bovine Serum (FBS)
*10% Fetal Bovine Serum (FBS)
*1% non-essential amino acids
*2 mM L-glutamine
*1 mM sodium pyruvate
*1500 mg/L sodium bicarbonate

Latest revision as of 23:18, 8 December 2014

U-87MG Cell Line[edit]

Back to Main

ATCC Website

Gene Expression (NCBI)

Morphology: Epithelial Cell

Cell Line Description: This is a hypodiploid human cell line with the modal chromosome number of 44 occurring in 48% of cells. The rate of higher ploidy was 5.9%., Twelve markers were common to all cells, including der(1)t(1;3) (p22;q21), der(16)t(1;16) (p22;p12), del(9) (p13) and nine others. The marker der(1) had two copies in most cells. There was only one copy of normal X. N1, N6 and N9 were not found.

Media:EMEM (EBSS)

  • 10% Fetal Bovine Serum (FBS)
  • 1% non-essential amino acids
  • 2 mM L-glutamine
  • 1 mM sodium pyruvate
  • 1500 mg/L sodium bicarbonate