Arichard:Notebook/midas150109: Difference between revisions

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==MIDAS January 09, 2015==
Overnight MDA 1/9 to 1/10
96 extractions 1/10, stored at 4 degC overnight
* Xenoworks INJECT line flooded with solution, leading to errors in the remote controller.
* Drierite also completely purple
Nextera and Ampure XP on 1/11
1st gel run on 1/12, random sample of 8 reactions. No positives.
Large amount of high-mass material in each lane, including empty lanes. Likely DNA contamination in TBE buffer.
Remaining gels run 1/16 with fresh buffer. No positives
[[File:midasGels150109Slide1.jpg|400px]]
[[File:midasGels150109Slide1.jpg|400px]]
[[File:midasGels150109Slide2.jpg|400px]]
[[File:midasGels150109Slide2.jpg|400px]]
[[File:midasGels150109Slide3.jpg|400px]]
[[File:midasGels150109Slide3.jpg|400px]]
[[File:midasGels150109Slide4.jpg|400px]]
[[File:midasGels150109Slide5.jpg|400px]]


[[File:midasGels150109Slide4.jpg|400px]]
No positives, same as last experiment. Need to run positive controls next time.


[[File:midasGels150109Slide5.jpg|400px]]
Regenerated drierite (200 degC for 1 hour)

Latest revision as of 18:11, 21 January 2015

MIDAS January 09, 2015[edit]

Overnight MDA 1/9 to 1/10

96 extractions 1/10, stored at 4 degC overnight

  • Xenoworks INJECT line flooded with solution, leading to errors in the remote controller.
  • Drierite also completely purple

Nextera and Ampure XP on 1/11

1st gel run on 1/12, random sample of 8 reactions. No positives. Large amount of high-mass material in each lane, including empty lanes. Likely DNA contamination in TBE buffer.

Remaining gels run 1/16 with fresh buffer. No positives

File:MidasGels150109Slide1.jpg File:MidasGels150109Slide2.jpg File:MidasGels150109Slide3.jpg File:MidasGels150109Slide4.jpg File:MidasGels150109Slide5.jpg

No positives, same as last experiment. Need to run positive controls next time.

Regenerated drierite (200 degC for 1 hour)