Arichard:Notebook/bulk150125: Difference between revisions

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==January 25, 2015 Bulk Nextera==
==January 25, 2015 Bulk Nextera==


Bulk Nextera from [http://genome-tech.ucsd.edu/LabNotes/index.php/Arichard:Notebook/bulk150122|January 22] worked on 2/3 samples (SW480 2N Full), so I decided to continue with all the SW480 (including the 2N Full again to get at least N = 3), as well as the lymphocytes (all sorted October 21, 2014).
Bulk Nextera from [http://genome-tech.ucsd.edu/LabNotes/index.php/Arichard:Notebook/bulk150122| January 22] worked on 2/3 samples (SW480 2N Full), so I decided to continue with all the SW480 (including the 2N Full again to get at least N = 3), as well as the lymphocytes (all sorted October 21, 2014).


[[File:bulk150125Slide1.jpg|400px]]
[[File:bulk150125Slide1.jpg|400px]]
[[File:bulk150125Slide2.jpg|400px]]
[[File:bulk150125Slide2.jpg|400px]]
[[File:bulk150125Slide3.jpg|400px]]
[[File:bulk150125Slide3.jpg|400px]]
[[File:bulk150125Slide4.jpg|400px]]
[[File:bulk150125Slide4.jpg|400px]]

Latest revision as of 01:31, 5 February 2015

January 25, 2015 Bulk Nextera[edit]

Bulk Nextera from January 22 worked on 2/3 samples (SW480 2N Full), so I decided to continue with all the SW480 (including the 2N Full again to get at least N = 3), as well as the lymphocytes (all sorted October 21, 2014).

File:Bulk150125Slide1.jpg File:Bulk150125Slide2.jpg

File:Bulk150125Slide3.jpg File:Bulk150125Slide4.jpg

Qiming likely added 2 barcodes to the same sample, so we need to figure out which is which. I will try to spike in the suspect libraries to different lanes to determine which barcodes belong to which libraries.

5 libraries ' 6 barcodes
QJ11-bulk-03Feb15-nxt14m 14 or 15
QJ12-bulk-03Feb15-nxt15m 15 or 16
QJ13-bulk-03Feb15-nxt16m 16 or 17
QJ14-bulk-03Feb15-nxt17m 17 or 18
QJ15-bulk-03Feb15-nxt18m 18 or 19

The m after the index (e.g., nxt14m) denotes "maybe", meaning that it could be either 14 or 15.