Blue:RNA-Seq Experiments:03062015: Difference between revisions

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(Created page with "== C1 Tube Controls == *Testing the quality of RNA for the following C1 runs: # 20150205A: Naac bulk sorted nuclei (MSSM) # 20130223A: GTEX Patient 10A Nuclei combined aliqu...")
 
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# 20130223B: GTEX Patient 10B Nuclei combined aliquots
# 20130223B: GTEX Patient 10B Nuclei combined aliquots


* RNA extracted using Zymo micro RNA kit
* RNA extracted using Zymo micro RNA kit (eluted in 6ul)
 
 
RNA Concentrations:
 
# Naac Bulk sort - 18.2ng/ul
# GTEX 10A Bulk Sort (combined 5 aliquots) - 5.17ng/ul
# GTEX 10B Bulk Sort (combined 5 aliquots) - 5.10ng/ul
 
Diluted RNA 1:10 and ran on Bioanalyzer using Pico chip:
 
*Blue1 = Naac RNA
*Blue2 = GTEX 10A RNA
*Blue3 = GTEX 10B RNA
*Blue4 = BA8 sort pool RNA ([[http://genome-tech.ucsd.edu/LabNotes/index.php/Blue:RNA-Seq_Experiments:04302014]]
 
[[media:RNA_Pico_2015-03-06_14-36-22.pdf]]

Latest revision as of 01:12, 7 March 2015

C1 Tube Controls[edit]

  • Testing the quality of RNA for the following C1 runs:
  1. 20150205A: Naac bulk sorted nuclei (MSSM)
  2. 20130223A: GTEX Patient 10A Nuclei combined aliquots
  3. 20130223B: GTEX Patient 10B Nuclei combined aliquots
  • RNA extracted using Zymo micro RNA kit (eluted in 6ul)


RNA Concentrations:

  1. Naac Bulk sort - 18.2ng/ul
  2. GTEX 10A Bulk Sort (combined 5 aliquots) - 5.17ng/ul
  3. GTEX 10B Bulk Sort (combined 5 aliquots) - 5.10ng/ul

Diluted RNA 1:10 and ran on Bioanalyzer using Pico chip:

  • Blue1 = Naac RNA
  • Blue2 = GTEX 10A RNA
  • Blue3 = GTEX 10B RNA
  • Blue4 = BA8 sort pool RNA ([[1]]

media:RNA_Pico_2015-03-06_14-36-22.pdf