Sam:LabNotes/Microbiome/2009-3-10: Difference between revisions

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>Sam Chiang
(New page: ='Perform MDA on human gDNA, diluted whole cells'''= ==Sample & Material== *Cell: GM18506 cell line, harvested, washed by UV-treated PBS and coundted to 200,000 cell/mL (200 cell/uL) **D...)
 
>Sam Chiang
(Removing all content from page)
 
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='Perform MDA on human gDNA, diluted whole cells'''=


==Sample & Material==
*Cell: GM18506 cell line, harvested, washed by UV-treated PBS and coundted to 200,000 cell/mL (200 cell/uL)
**Dilute to 10 cell/uL, 5 cell/uL, 0.5 cell/uL using UV-treated PBS
  Dilute factor    original    x 1/2  x 1/10  x 1/2    x 1/10
  conc.(cell/uL)      200/uL  100/uL    10/uL    5/uL    0.5/uL
  Transfering(uL)          50        10      50      10                   
  UVed-PBS(uL)                  50      90      50      90
                           
*Pos. control: human gDNA diluted to 3 ng/uL
**Measure the original (commercial human gDNA) using Nanodrop -> 309ng/uL
  Dilute factor    original      x 1/10      x 1/10    x 1/10    x 1/10
  conc.            309ng/uL      3ng/uL    300pg/uL    30pg/uL    3pg/uL
  Transfering(uL)          10          10          10        10                   
  UVed-PBS(uL)                    90          90        90        90

Latest revision as of 17:17, 18 March 2009