Daniel:Notebook/RNAFACS/2015-10-23: Difference between revisions

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This is a good result.  The field 1 and field 2 images show some nuclei with both FEZF2 and NeuN signal, whereas field 3 only has NeuN.  We expect to see a large number of nuclei with signal in NeuN and fewer with FEZF2.
This is a good result.  The field 1 and field 2 images show some nuclei with both FEZF2 and NeuN signal, whereas field 3 only has NeuN.  We expect to see a large number of nuclei with signal in NeuN and fewer with FEZF2.


[[Category:RNAScope]] [[Category:Microscopy]] [[Category:RNAFACS]]
[[Category:RNAScope]] [[Category:Microscopy]] [[Category:RNAFACS]] [[Category:PretreatII]]

Latest revision as of 18:37, 27 October 2015

RNA Scope Trial Run 5: Pretreat II (Started 10/22/2015)[edit]

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Microscopy Results[edit]

Here are the microscopy results from the trial today:

For future use: using line average (when signal is strong) can reduce the amount of background noise seen. The field 1 image has the most background and the highest threshold (~50 for the Green/Yellow), whereas field 2 and field 3 only had around 20 as the intensity threshold (same gain, 750, for all fields/channels).

This is a good result. The field 1 and field 2 images show some nuclei with both FEZF2 and NeuN signal, whereas field 3 only has NeuN. We expect to see a large number of nuclei with signal in NeuN and fewer with FEZF2.