Daniel:Notebook/ComboLock: Difference between revisions

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[[Daniel Jacobsen|Back to Main]]
[[Daniel Jacobsen|Back to Main]]


This page describes experiments done for the Combo-lock project. The overview of the project is to encapsulate cells (or nuclei) in droplets using a Drop Seq type device. The device will include bis/acrylamide gel formation, and formaldehyde to bond the nucleotides/protein to e gel matrix. following that, attachment of barcoded RNA/antibodyprobes will occur. The antibodies will contain nucleotide barcodes. The nucleotide barcodes will be read by universl pdlock probes, which will then be ligated/cicularized. following ligation, RCA will be performed, and the RCA product isolated for sequencing.
This page describes experiments done for the Combo-lock project. The overview of the project is to encapsulate cells (or nuclei) in droplets using a Drop Seq type device. The device will include bis/acrylamide gel formation, and formaldehyde to bond the nucleotides/protein to e gel matrix. following that, attachment of barcoded RNA/antibody probes will occur. The antibodies will contain nucleotide barcodes. The nucleotide barcodes will be read by universal padlock probes, which will then be ligated/cicularized. following ligation, RCA will be performed, and the RCA product isolated for sequencing.


==Assorted Lab Notes==
==Assorted Lab Notes==
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[[Daniel:Notebook/ComboLock/CProbeDesign|C Probe Design]]
[[Daniel:Notebook/ComboLock/CProbeDesign|C Probe Design]]
[[Daniel:Notebook/ComboLock/ExperimentList|Experiment List]]
[[Daniel:Notebook/ComboLock/Sequencing|Sequencing Lists]]


==Calendar==
==Calendar==
<calendar>
name=Daniel:Notebook/ComboLock
format=%name/%year-%month-%day
date=2017/02/01
view=oneyear
</calendar>


<calendar>
<calendar>

Latest revision as of 23:42, 5 January 2017

Combo-Lock[edit]

Back to Main

This page describes experiments done for the Combo-lock project. The overview of the project is to encapsulate cells (or nuclei) in droplets using a Drop Seq type device. The device will include bis/acrylamide gel formation, and formaldehyde to bond the nucleotides/protein to e gel matrix. following that, attachment of barcoded RNA/antibody probes will occur. The antibodies will contain nucleotide barcodes. The nucleotide barcodes will be read by universal padlock probes, which will then be ligated/cicularized. following ligation, RCA will be performed, and the RCA product isolated for sequencing.

Assorted Lab Notes[edit]

IP Presentations

PA Gel Formulas

Microfluidic Devices

C Probe Design

Experiment List

Sequencing Lists

Calendar[edit]

<calendar> name=Daniel:Notebook/ComboLock format=%name/%year-%month-%day date=2017/02/01 view=oneyear </calendar>

<calendar> name=Daniel:Notebook/ComboLock format=%name/%year-%month-%day date=2016/02/01 view=oneyear </calendar>