Daniel:Notebook/ComboLock/2016-6-25: Difference between revisions

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(Created page with "=Stage 2 Test (Started yesterday)= Back to Calendar ==Protocol== <ol start="7"> <li>Ethanol Precipitat...")
 
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| height="15" | Batch2
| height="15" | Batch2
| align="center" | &nbsp;
| align="center" | 43.1


|- style="background-color:#D9D9D9;font-size:12pt"  valign="bottom"
|- style="background-color:#D9D9D9;font-size:12pt"  valign="bottom"
| height="15" | Batch3
| height="15" | Batch3
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| align="center" |11.1


|- style="font-size:12pt"  valign="bottom"
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| height="15" | Batch5
| align="center" | &nbsp;
| align="center" | 36.1


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| height="15" | Batch6
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| align="center" |18.3


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[[Category:ComboLock]] [[Category:20160625]]

Latest revision as of 22:27, 6 July 2016

Stage 2 Test (Started yesterday)[edit]

Back to Calendar

Protocol[edit]

  1. Ethanol Precipitation
    1. Centrifuge at 4 C for 25 minutes at 14000 rpm
    2. Remove supernatant and add 750 uL chilled 75% EtOH
    3. Centrifuge for 15 minutes at minutes at 4 C at 14000 rpm
    4. Remove supernatant and dry the pellet in the hood
    5. Resuspend pellet in 20 uL volume nfH20
    6. Measure in Nanodrop
    7. Store DNA at 4C

Nanodrop Results[edit]

Batch Source ng/uL
Batch2 43.1
Batch3 11.1
Batch5 36.1
Batch6 18.3