Daniel:Notebook/ComboLock/2016-6-25: Difference between revisions
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[[Category:ComboLock]] [[Category:20160625]] |
Latest revision as of 22:27, 6 July 2016
Stage 2 Test (Started yesterday)[edit]
Protocol[edit]
- Ethanol Precipitation
- Centrifuge at 4 C for 25 minutes at 14000 rpm
- Remove supernatant and add 750 uL chilled 75% EtOH
- Centrifuge for 15 minutes at minutes at 4 C at 14000 rpm
- Remove supernatant and dry the pellet in the hood
- Resuspend pellet in 20 uL volume nfH20
- Measure in Nanodrop
- Store DNA at 4C
Nanodrop Results[edit]
Batch Source | ng/uL |
Batch2 | 43.1 |
Batch3 | 11.1 |
Batch5 | 36.1 |
Batch6 | 18.3 |