Daniel:Notebook/ComboLock/2016-7-27: Difference between revisions

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=Latch 100 and Dextran Test (Started [[Daniel:Notebook/ComboLock/2016-7-25|7-25-2016]]=
=Latch 100 and Dextran Test (Started [[Daniel:Notebook/ComboLock/2016-7-25|7-25-2016]])=


[[Daniel:Notebook/ComboLock|Back to Calendar]]
[[Daniel:Notebook/ComboLock|Back to Calendar]]


==Library Prep (from [[Daniel:Notebook/ComboLock/2016-7-26|yesterday]]==
==Library Prep (from [[Daniel:Notebook/ComboLock/2016-7-26|yesterday]])==


<ol start="6">
<ol start="6">
<li>Ethanol precipitation (from [[Daniel:Notebook/ComboLock/2016-7-12|yesterday]])</li>
<li>Ethanol precipitation</li>
<ol type="A" start="3">
<ol type="A" start="3">
<li>Centrifuge at 4 C for 25 minutes at 14000 rpm
<li>Centrifuge at 4 C for 25 minutes at 14000 rpm
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{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext>
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext>
|- style="background-color:#DDD9C4;font-size:12pt;font-weight:bold" align="center"
|- style="background-color:#DDD9C4;font-size:12pt;font-weight:bold" align="center"
| width="106" height="30" | Sample
| width="76" height="30" | Sample
| width="65" | ng/uL
| width="65" | ng/uL
| width="65" | uL added
| width="65" | uL added
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|- style="font-size:12pt"
|- style="font-size:12pt"
| height="15"  | 7-21 Sample A
| height="30"  | 7-26 Sample A
| align="center" align="center" | 44.5
| align="center" align="center" | 31
| align="center" align="center" | 15
| align="center" align="center" | 15
| align="center" align="center" | 667.5
| align="center" align="center" | 465


|- style="background-color:#D9D9D9;font-size:12pt"
|- style="background-color:#D9D9D9;font-size:12pt"
| height="15"  | 7-21 Sample B
| height="30"  | 7-26 Sample B
| align="center" align="center" | 13.9
| align="center" align="center" | 19.3
| align="center" align="center" valign="bottom" | 15
| align="center" align="center" | 15
| align="center" align="center" valign="bottom" | 208.5
| align="center" align="center" | 289.5


|}
|}
[[Category:ComboLock]] [[Category:20160725]]

Latest revision as of 16:24, 28 July 2016

Latch 100 and Dextran Test (Started 7-25-2016)[edit]

Back to Calendar

Library Prep (from yesterday)[edit]

  1. Ethanol precipitation
    1. Centrifuge at 4 C for 25 minutes at 14000 rpm
    2. Remove supernatant and add 750 uL chilled 75% EtOH
    3. Centrifuge for 15 minutes at minutes at 4 C at 14000 rpm
    4. Remove supernatant and dry the pellet in the hood
    5. Resuspend pellet in 20 uL volume nfH20
    6. Measure in Nanodrop
    7. Combined 15 uL from each sample
    8. Store DNA at 4C

Nanodrop Results[edit]

Source ng/uL
7-26 (Latch100/Dextran) A 31.0
7-26 (Latch100/Dextran) B 19.3
Sample ng/uL uL added final mass (ng)
7-26 Sample A 31 15 465
7-26 Sample B 19.3 15 289.5