Daniel:Notebook/ComboLock/2016-7-27: Difference between revisions
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=Latch 100 and Dextran Test (Started [[Daniel:Notebook/ComboLock/2016-7-25|7-25-2016]]= | =Latch 100 and Dextran Test (Started [[Daniel:Notebook/ComboLock/2016-7-25|7-25-2016]])= | ||
[[Daniel:Notebook/ComboLock|Back to Calendar]] | [[Daniel:Notebook/ComboLock|Back to Calendar]] | ||
==Library Prep (from [[Daniel:Notebook/ComboLock/2016-7-26|yesterday]]== | ==Library Prep (from [[Daniel:Notebook/ComboLock/2016-7-26|yesterday]])== | ||
<ol start="6"> | <ol start="6"> | ||
<li>Ethanol precipitation | <li>Ethanol precipitation</li> | ||
<ol type="A" start="3"> | <ol type="A" start="3"> | ||
<li>Centrifuge at 4 C for 25 minutes at 14000 rpm | <li>Centrifuge at 4 C for 25 minutes at 14000 rpm | ||
Line 37: | Line 37: | ||
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext> | {| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext> | ||
|- style="background-color:#DDD9C4;font-size:12pt;font-weight:bold" align="center" | |- style="background-color:#DDD9C4;font-size:12pt;font-weight:bold" align="center" | ||
| width=" | | width="76" height="30" | Sample | ||
| width="65" | ng/uL | | width="65" | ng/uL | ||
| width="65" | uL added | | width="65" | uL added | ||
Line 43: | Line 43: | ||
|- style="font-size:12pt" | |- style="font-size:12pt" | ||
| height=" | | height="30" | 7-26 Sample A | ||
| align="center" align="center" | | | align="center" align="center" | 31 | ||
| align="center" align="center" | 15 | | align="center" align="center" | 15 | ||
| align="center" align="center" | | | align="center" align="center" | 465 | ||
|- style="background-color:#D9D9D9;font-size:12pt" | |- style="background-color:#D9D9D9;font-size:12pt" | ||
| height=" | | height="30" | 7-26 Sample B | ||
| align="center" align="center" | | | align="center" align="center" | 19.3 | ||
| align="center" align="center | | align="center" align="center" | 15 | ||
| align="center" align="center | | align="center" align="center" | 289.5 | ||
|} | |} | ||
[[Category:ComboLock]] [[Category:20160725]] |
Latest revision as of 16:24, 28 July 2016
Latch 100 and Dextran Test (Started 7-25-2016)[edit]
Library Prep (from yesterday)[edit]
- Ethanol precipitation
- Centrifuge at 4 C for 25 minutes at 14000 rpm
- Remove supernatant and add 750 uL chilled 75% EtOH
- Centrifuge for 15 minutes at minutes at 4 C at 14000 rpm
- Remove supernatant and dry the pellet in the hood
- Resuspend pellet in 20 uL volume nfH20
- Measure in Nanodrop
- Combined 15 uL from each sample
- Store DNA at 4C
Nanodrop Results[edit]
Source | ng/uL |
7-26 (Latch100/Dextran) A | 31.0 |
7-26 (Latch100/Dextran) B | 19.3 |
Sample | ng/uL | uL added | final mass (ng) |
7-26 Sample A | 31 | 15 | 465 |
7-26 Sample B | 19.3 | 15 | 289.5 |