Daniel:Notebook/ComboLock/2016-9-6: Difference between revisions
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<li>Combined 15 uL from each of the two tubes above</li> | <li>Combined 15 uL from each of the two tubes above</li> | ||
<li>Stored at 4C in upcoming libraries box</li> | <li>Stored at 4C in upcoming libraries box</li> | ||
<li>Sample '''DEJ-CL-PC03'''</li> | |||
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[[Category:ComboLock]] [[Category:20160829]] |
Latest revision as of 20:13, 12 September 2016
X3 Dilution Test (8/29/2016)[edit]
Protocol[edit]
- Ethanol Precipitation
- Pellet by centrifuging for 25 minutes at 12000rpm at 4C
- Remove supernatant and add 750 uL chilled 70% EtOH
- Centrifuge for 12 minutes at 12000rpm at 4C
- Remove supernatant and spin in vacuum centrifuge for 10 minutes
- Add 20 uL nfH2O and measure in nanodrop
- Combined 15 uL from each of the two tubes above
- Stored at 4C in upcoming libraries box
- Sample DEJ-CL-PC03
Nanodrop Results[edit]
Sample | ng/uL |
Sample A | 31 |
Sample B | 19.3 |
Sequencing Library[edit]
Sample | ng/uL | uL added | final mass (ng) |
9-6 Sample A | 31 | 15 | 465 |
9-6 Sample B | 19.3 | 15 | 289.5 |