Daniel:Notebook/ComboLock/2016-10-20: Difference between revisions

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(Created page with "=Padlock0301 Test (Started 10/07/2016)= Back to Calendar ==Sequencing Results==")
 
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==Sequencing Results==
==Sequencing Results==
Results from the 10/18 miseq. Recall that Idx24 (ATTCGC) is the experiment index and Idx25 (TACTGC) is the NTC index.
===Alignment Results===
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext>
|- style="background-color:#E6B8B7;font-size:12pt"
| align="center" width="171" height="30"  valign="bottom" | &nbsp;
|style="font-weight:bold" width="65" align="center" | Length
|style="font-weight:bold" width="53" align="center" | Idx 24 Hits
|style="font-weight:bold" width="61" align="center" | Idx24 % Reads
|style="font-weight:bold" width="65" align="center" | Idx 25 Reads
|style="font-weight:bold" width="65" align="center" | Idx 25 % Reads
|- style="font-size:12pt"  valign="bottom"
|style="font-weight:bold" height="15" | PosConAmpC1_C2
| align="center" | 160
| align="center" | 0
| align="center" | 0.0
| align="center" | 0
| align="center" | 0.0
|- style="background-color:#BFBFBF;font-size:12pt"  valign="bottom"
|style="font-weight:bold" height="15" | PosConAmpC3_C2
| align="center" | 160
| align="center" | 0
| align="center" | 0.0
| align="center" | 16
| align="center" | 32.7
|- style="font-size:12pt"  valign="bottom"
|style="font-weight:bold" height="15" | PosConAmpC1_C2_RevComp
| align="center" | 160
| align="center" | 0
| align="center" | 0.0
| align="center" | 0
| align="center" | 0.0
|- style="background-color:#BFBFBF;font-size:12pt"  valign="bottom"
|style="font-weight:bold" height="15" | PosConAmpC3_C2_RevComp
| align="center" | 160
| align="center" | 0
| align="center" | 0.0
| align="center" | 15
| align="center" | 30.6
|- style="font-size:12pt"  valign="bottom"
|style="font-weight:bold" height="15" | PosConPadlock0301_RevComp
| align="center" | 147
| align="center" | 133
| align="center" | 12.7
| align="center" | 0
| align="center" | 0.0
|- style="background-color:#BFBFBF;font-size:12pt"  valign="bottom"
|style="font-weight:bold" height="15" | PosConPadlock0201_RevComp
| align="center" | 152
| align="center" | 171
| align="center" | 16.3
| align="center" | 0
| align="center" | 0.0
|}
===Base Quality===
<gallery perrow=2 heights=300px widths=300px>
File:Miseq20161018.idx24.exp.basequalityhistogram.png|Index 24 (Experiment) Read Quality
File:Miseq20161018.idx25.ntc.basequality.png|Index 25 (NTC) Read Quality
</gallery>
===Amplification Primers Gel===
This is a gel I ran to test the "cleanliness" of the amplification primers (no qPCR). The recipe for each lane is 2 uL (10 uM) sample, 8 uL TBE, 2 uL 6x dye, 10 uL added to lane.
[[Image:2016-10-20-AmplificationPrimers.png|600px]]
[[Category:ComboLock]] [[Category:20161007]]

Latest revision as of 18:19, 24 October 2016

Padlock0301 Test (Started 10/07/2016)[edit]

Back to Calendar

Sequencing Results[edit]

Results from the 10/18 miseq. Recall that Idx24 (ATTCGC) is the experiment index and Idx25 (TACTGC) is the NTC index.

Alignment Results[edit]

  Length Idx 24 Hits Idx24 % Reads Idx 25 Reads Idx 25 % Reads
PosConAmpC1_C2 160 0 0.0 0 0.0
PosConAmpC3_C2 160 0 0.0 16 32.7
PosConAmpC1_C2_RevComp 160 0 0.0 0 0.0
PosConAmpC3_C2_RevComp 160 0 0.0 15 30.6
PosConPadlock0301_RevComp 147 133 12.7 0 0.0
PosConPadlock0201_RevComp 152 171 16.3 0 0.0

Base Quality[edit]

Amplification Primers Gel[edit]

This is a gel I ran to test the "cleanliness" of the amplification primers (no qPCR). The recipe for each lane is 2 uL (10 uM) sample, 8 uL TBE, 2 uL 6x dye, 10 uL added to lane.

File:2016-10-20-AmplificationPrimers.png