Daniel:Notebook/ComboLock/2016-10-20: Difference between revisions
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>Djacobse (Created page with "=Padlock0301 Test (Started 10/07/2016)= Back to Calendar ==Sequencing Results==") |
>Djacobse |
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==Sequencing Results== | ==Sequencing Results== | ||
Results from the 10/18 miseq. Recall that Idx24 (ATTCGC) is the experiment index and Idx25 (TACTGC) is the NTC index. | |||
===Alignment Results=== | |||
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext> | |||
|- style="background-color:#E6B8B7;font-size:12pt" | |||
| align="center" width="171" height="30" valign="bottom" | | |||
|style="font-weight:bold" width="65" align="center" | Length | |||
|style="font-weight:bold" width="53" align="center" | Idx 24 Hits | |||
|style="font-weight:bold" width="61" align="center" | Idx24 % Reads | |||
|style="font-weight:bold" width="65" align="center" | Idx 25 Reads | |||
|style="font-weight:bold" width="65" align="center" | Idx 25 % Reads | |||
|- style="font-size:12pt" valign="bottom" | |||
|style="font-weight:bold" height="15" | PosConAmpC1_C2 | |||
| align="center" | 160 | |||
| align="center" | 0 | |||
| align="center" | 0.0 | |||
| align="center" | 0 | |||
| align="center" | 0.0 | |||
|- style="background-color:#BFBFBF;font-size:12pt" valign="bottom" | |||
|style="font-weight:bold" height="15" | PosConAmpC3_C2 | |||
| align="center" | 160 | |||
| align="center" | 0 | |||
| align="center" | 0.0 | |||
| align="center" | 16 | |||
| align="center" | 32.7 | |||
|- style="font-size:12pt" valign="bottom" | |||
|style="font-weight:bold" height="15" | PosConAmpC1_C2_RevComp | |||
| align="center" | 160 | |||
| align="center" | 0 | |||
| align="center" | 0.0 | |||
| align="center" | 0 | |||
| align="center" | 0.0 | |||
|- style="background-color:#BFBFBF;font-size:12pt" valign="bottom" | |||
|style="font-weight:bold" height="15" | PosConAmpC3_C2_RevComp | |||
| align="center" | 160 | |||
| align="center" | 0 | |||
| align="center" | 0.0 | |||
| align="center" | 15 | |||
| align="center" | 30.6 | |||
|- style="font-size:12pt" valign="bottom" | |||
|style="font-weight:bold" height="15" | PosConPadlock0301_RevComp | |||
| align="center" | 147 | |||
| align="center" | 133 | |||
| align="center" | 12.7 | |||
| align="center" | 0 | |||
| align="center" | 0.0 | |||
|- style="background-color:#BFBFBF;font-size:12pt" valign="bottom" | |||
|style="font-weight:bold" height="15" | PosConPadlock0201_RevComp | |||
| align="center" | 152 | |||
| align="center" | 171 | |||
| align="center" | 16.3 | |||
| align="center" | 0 | |||
| align="center" | 0.0 | |||
|} | |||
===Base Quality=== | |||
<gallery perrow=2 heights=300px widths=300px> | |||
File:Miseq20161018.idx24.exp.basequalityhistogram.png|Index 24 (Experiment) Read Quality | |||
File:Miseq20161018.idx25.ntc.basequality.png|Index 25 (NTC) Read Quality | |||
</gallery> | |||
===Amplification Primers Gel=== | |||
This is a gel I ran to test the "cleanliness" of the amplification primers (no qPCR). The recipe for each lane is 2 uL (10 uM) sample, 8 uL TBE, 2 uL 6x dye, 10 uL added to lane. | |||
[[Image:2016-10-20-AmplificationPrimers.png|600px]] | |||
[[Category:ComboLock]] [[Category:20161007]] |
Latest revision as of 18:19, 24 October 2016
Padlock0301 Test (Started 10/07/2016)[edit]
Sequencing Results[edit]
Results from the 10/18 miseq. Recall that Idx24 (ATTCGC) is the experiment index and Idx25 (TACTGC) is the NTC index.
Alignment Results[edit]
Length | Idx 24 Hits | Idx24 % Reads | Idx 25 Reads | Idx 25 % Reads | |
PosConAmpC1_C2 | 160 | 0 | 0.0 | 0 | 0.0 |
PosConAmpC3_C2 | 160 | 0 | 0.0 | 16 | 32.7 |
PosConAmpC1_C2_RevComp | 160 | 0 | 0.0 | 0 | 0.0 |
PosConAmpC3_C2_RevComp | 160 | 0 | 0.0 | 15 | 30.6 |
PosConPadlock0301_RevComp | 147 | 133 | 12.7 | 0 | 0.0 |
PosConPadlock0201_RevComp | 152 | 171 | 16.3 | 0 | 0.0 |
Base Quality[edit]
- Miseq20161018.idx24.exp.basequalityhistogram.png
Index 24 (Experiment) Read Quality
- Miseq20161018.idx25.ntc.basequality.png
Index 25 (NTC) Read Quality
Amplification Primers Gel[edit]
This is a gel I ran to test the "cleanliness" of the amplification primers (no qPCR). The recipe for each lane is 2 uL (10 uM) sample, 8 uL TBE, 2 uL 6x dye, 10 uL added to lane.