Daniel:Notebook/ComboLock/2017-1-10: Difference between revisions
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'''DEJ20170109-CLv2-PC''' | '''DEJ20170109-CLv2-PC''' | ||
[[Category:ComboLock]] [[Category:20170106]] |
Latest revision as of 21:55, 13 January 2017
Version 2 Oligos (Started 1/6/2017)[edit]
Library Prep (Started Yesterday)[edit]
- Ethanol Precipitation
- Centrifuge for 25 minutes at 12000 rpm at 4C
- Remove supernatant and add 750 uL chilled 70% EtOH
- Centrifuge for 12 minutes at 12000 rpm at 4C
- Remove supernatant and spin in vacuum centrifuge for 10 minutes
- Add 30 uL nfH2O to resuspend; pool samples A and B by reusing the water
Sample | ng/uL | ug in 30 uL |
Sample Lanes (1A+1B) | 40.3 | 1.2 |
DEJ20170109-CLv2-PC