Daniel:Notebook/ComboLock/2017-1-10: Difference between revisions

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'''DEJ20170109-CLv2-PC'''
'''DEJ20170109-CLv2-PC'''
[[Category:ComboLock]] [[Category:20170106]]

Latest revision as of 21:55, 13 January 2017

Version 2 Oligos (Started 1/6/2017)[edit]

Back to Calendar

Library Prep (Started Yesterday)[edit]

  1. Ethanol Precipitation
    1. Centrifuge for 25 minutes at 12000 rpm at 4C
    2. Remove supernatant and add 750 uL chilled 70% EtOH
    3. Centrifuge for 12 minutes at 12000 rpm at 4C
    4. Remove supernatant and spin in vacuum centrifuge for 10 minutes
    5. Add 30 uL nfH2O to resuspend; pool samples A and B by reusing the water
Sample ng/uL ug in 30 uL
Sample Lanes (1A+1B) 40.3 1.2

DEJ20170109-CLv2-PC