AlanFung:Protocol/Cell Counting: Difference between revisions
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Suspend Cells | Suspend Cells | ||
Perform Cell Count | Perform Cell Count | ||
*Protocol | |||
Prime Amperature | |||
Turn on cell counter | |||
Fill small cup with 10mL balance solution | |||
Replace cleaning solution cup with | |||
*Prepare cups with 10ml balance solution(1cup/sample) | |||
*Prepare single cell suspension by repeat pipetting | |||
*Aspirate 100uL of single cell suspension into cup | |||
*Swirl cup | |||
*Place cup into holder | |||
*Hit <Setup> then <Enter> |
Latest revision as of 01:03, 17 June 2009
Cell Counting
- Material
Cell counter Small cup Balance solution 5mL pipette Pipette aid P200 pipette P200 pipette tips
- Overview
Prime Amperture Suspend Cells Perform Cell Count
- Protocol
Prime Amperature
Turn on cell counter Fill small cup with 10mL balance solution Replace cleaning solution cup with
- Prepare cups with 10ml balance solution(1cup/sample)
- Prepare single cell suspension by repeat pipetting
- Aspirate 100uL of single cell suspension into cup
- Swirl cup
- Place cup into holder
- Hit <Setup> then <Enter>