Daniel:Notebook/ComboLock/2017-6-20: Difference between revisions
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= | =Antibodies Test (Started [[Daniel:Notebook/ComboLock/2017-6-14|Wednesday 6/14]])= | ||
[[Daniel:Notebook/ComboLock|Back to Calendar]] | [[Daniel:Notebook/ComboLock|Back to Calendar]] |
Latest revision as of 22:44, 28 June 2017
Antibodies Test (Started Wednesday 6/14)[edit]
Library Prep (Started Yesterday)[edit]
- Ethanol Precipitation
- Remove from -80C and centrifuge for 30 minutes at 12000rpm at 4C
- Remove supernatant and add 700 uL chilled 70% EtOH
- Centrifuge for 12 minutes at 12000rpm at 4C
- Remove supernatant and dry in vacuum centrifuge for 10 minutes
- Resuspend and pool samples in 30 uL nfH2O
- Qubit Measurement
- Mix 597 uL Qubit buffer with 3 uL dye
- For standards, add 190 uL buffer mix and 10 uL standard to a Qubit tube
- For sample, add 199 uL buffer mix and 1 uL sample to a Qubit tube
- Let sit in the dark 5 minutes
- Measure in Qubit using new standards
Library DEJ20170610-CLAbPC-01
1.22 ug/mL