Daniel:Notebook/ComboLock/2017-7-11: Difference between revisions
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>Djacobse (Created page with "=Oligo+Protein Test (Started Thursday July 6)= Back to Calendar ==Library Prep (Started Daniel:Notebo...") |
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[[Daniel:Notebook/ComboLock|Back to Calendar]] | [[Daniel:Notebook/ComboLock|Back to Calendar]] | ||
==Library Prep (Started [[Daniel:Notebook/ComboLock/2017-7-10|Yesterday]])== | ==Library Prep (Started [[Daniel:Notebook/ComboLock/2017-7-10|Yesterday]])== | ||
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<li>Ethanol Precipitation</li> | <li>Ethanol Precipitation</li> | ||
<ol type="A"> | <ol type="A"> | ||
<li>Remove from -80C and centrifuge for 30 minutes at 12000rpm at 4C</li> | |||
<li>Remove supernatant and add 700 uL chilled 70% EtOH</li> | |||
<li>Centrifuge for 12 minutes at 12000rpm at 4C</li> | |||
<li>Remove supernatant and dry in vacuum centrifuge for 10 minutes</li> | |||
<li>Resuspend and pool samples in 35 uL nfH<sub>2</sub>O</li> | |||
</ol> | |||
<li>Qubit Measurement</li> | |||
<ol type="A"> | |||
<li>Mix 597 uL Qubit buffer with 3 uL dye</li> | |||
<li>For standards, add 190 uL buffer mix and 10 uL standard to a Qubit tube</li> | |||
<li>For sample, add 199 uL buffer mix and 1 uL sample to a Qubit tube</li> | |||
<li>Let sit in the dark 5 minutes</li> | |||
<li>Measure in Qubit using new standards</li> | |||
</ol> | </ol> | ||
</ol> | </ol> | ||
'''DEJ20170711-MixPC-01'''5.13 ug/mL (ng/uL) | '''DEJ20170711-MixPC-01''': 5.13 ug/mL (ng/uL) |
Latest revision as of 18:27, 11 July 2017
Oligo+Protein Test (Started Thursday July 6)[edit]
Library Prep (Started Yesterday)[edit]
- Ethanol Precipitation
- Remove from -80C and centrifuge for 30 minutes at 12000rpm at 4C
- Remove supernatant and add 700 uL chilled 70% EtOH
- Centrifuge for 12 minutes at 12000rpm at 4C
- Remove supernatant and dry in vacuum centrifuge for 10 minutes
- Resuspend and pool samples in 35 uL nfH2O
- Qubit Measurement
- Mix 597 uL Qubit buffer with 3 uL dye
- For standards, add 190 uL buffer mix and 10 uL standard to a Qubit tube
- For sample, add 199 uL buffer mix and 1 uL sample to a Qubit tube
- Let sit in the dark 5 minutes
- Measure in Qubit using new standards
DEJ20170711-MixPC-01: 5.13 ug/mL (ng/uL)