Daniel:Notebook/ComboLock/2017-7-11: Difference between revisions
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[[Daniel:Notebook/ComboLock|Back to Calendar]] | [[Daniel:Notebook/ComboLock|Back to Calendar]] | ||
==Library Prep (Started [[Daniel:Notebook/ComboLock/2017-7-10|Yesterday]])== | ==Library Prep (Started [[Daniel:Notebook/ComboLock/2017-7-10|Yesterday]])== |
Latest revision as of 18:27, 11 July 2017
Oligo+Protein Test (Started Thursday July 6)[edit]
Library Prep (Started Yesterday)[edit]
- Ethanol Precipitation
- Remove from -80C and centrifuge for 30 minutes at 12000rpm at 4C
- Remove supernatant and add 700 uL chilled 70% EtOH
- Centrifuge for 12 minutes at 12000rpm at 4C
- Remove supernatant and dry in vacuum centrifuge for 10 minutes
- Resuspend and pool samples in 35 uL nfH2O
- Qubit Measurement
- Mix 597 uL Qubit buffer with 3 uL dye
- For standards, add 190 uL buffer mix and 10 uL standard to a Qubit tube
- For sample, add 199 uL buffer mix and 1 uL sample to a Qubit tube
- Let sit in the dark 5 minutes
- Measure in Qubit using new standards
DEJ20170711-MixPC-01: 5.13 ug/mL (ng/uL)