Alice:LabNotes/2009-5-29: Difference between revisions
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>Zsakura2 (New page: ===PCR with AmpF/R6.3Sol for end sequencing (done by Ida)=== detailed information on the templates can be found here:[[http://genome-tech.ucsd.edu/LabNotes/index.php/Alice:LabNotes/2009-5...) |
>Zsakura2 |
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Line 16: | Line 16: | ||
TBE gel result: | TBE gel result: | ||
[[Image:ZhangLab_2 2009-05-29 11hr 43min.jpg]] | [[Image:ZhangLab_2 2009-05-29 11hr 43min.jpg]] | ||
Qiaquick column purification and elute in 30ul of ddH2O. | |||
Nanodrop: | |||
b: 22ng/ul | |||
c: 25ng/ul | |||
The samples were then sent to Gao Yuan for end-sequencing: | |||
b - PGP2_052909A: 111nM x 15ul | |||
c - PGP3_052909A: 126nM x 15ul |
Latest revision as of 23:35, 29 May 2009
PCR with AmpF/R6.3Sol for end sequencing (done by Ida)[edit]
detailed information on the templates can be found here:[[1]] b - exome probe sets captured with PGP2 (0.25ng/ul) c - exome probe sets captured with PGP3 (0.26ng/ul) x2 Template (b/c) 1ul 2X iProof Mastermix 50ul 100ul AmpF6.3SoL (10uM) 4ul 8ul AmpR6.3SoL (10uM) 4ul 8ul 50X SYBG I 0.8ul 1.6ul H2O 41.2ul 82.4ul 98C 30S -> (98C 10S -> 58C 20S -> 72C 20S) x 4 -> (98C 10S -> 72C 20S) x 5 -> 72C 3 min -> 15C hold.
TBE gel result: File:ZhangLab 2 2009-05-29 11hr 43min.jpg
Qiaquick column purification and elute in 30ul of ddH2O. Nanodrop: b: 22ng/ul c: 25ng/ul
The samples were then sent to Gao Yuan for end-sequencing: b - PGP2_052909A: 111nM x 15ul c - PGP3_052909A: 126nM x 15ul