Alice:LabNotes/2009-5-29: Difference between revisions

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>Zsakura2
(New page: ===PCR with AmpF/R6.3Sol for end sequencing (done by Ida)=== detailed information on the templates can be found here:[[http://genome-tech.ucsd.edu/LabNotes/index.php/Alice:LabNotes/2009-5...)
 
>Zsakura2
 
Line 16: Line 16:
  TBE gel result:
  TBE gel result:
  [[Image:ZhangLab_2 2009-05-29 11hr 43min.jpg]]
  [[Image:ZhangLab_2 2009-05-29 11hr 43min.jpg]]
Qiaquick column purification and elute in 30ul of ddH2O.
Nanodrop:
b: 22ng/ul
c: 25ng/ul
The samples were then sent to Gao Yuan for end-sequencing:
b - PGP2_052909A: 111nM x 15ul
c - PGP3_052909A: 126nM x 15ul

Latest revision as of 23:35, 29 May 2009

PCR with AmpF/R6.3Sol for end sequencing (done by Ida)[edit]

detailed information on the templates can be found here:[[1]]

b - exome probe sets captured with PGP2 (0.25ng/ul)
c - exome probe sets captured with PGP3 (0.26ng/ul)
                                      x2
   Template (b/c)           1ul      
   2X iProof Mastermix     50ul      100ul
   AmpF6.3SoL (10uM)        4ul       8ul
   AmpR6.3SoL (10uM)        4ul       8ul   
   50X SYBG I             0.8ul      1.6ul
   H2O                   41.2ul     82.4ul
   
98C 30S -> (98C 10S -> 58C 20S -> 72C 20S) x 4 -> (98C 10S -> 72C 20S) x 5 -> 72C 3 min -> 15C hold.
TBE gel result:
File:ZhangLab 2 2009-05-29 11hr 43min.jpg
Qiaquick column purification and elute in 30ul of ddH2O.
Nanodrop:
b: 22ng/ul
c: 25ng/ul
The samples were then sent to Gao Yuan for end-sequencing:
b - PGP2_052909A: 111nM x 15ul
c - PGP3_052909A: 126nM x 15ul