Kun:LabNotes/ExonomeSeq/2007-12-23: Difference between revisions
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(New page: ==Exp.1 PCR on the capturing reaction started on 12/21== Template: 10ul 10X JumpStart buffer: 9ul 25mM dNTP 1ul 100uM AmpFV6.2Sol 0.4ul...) |
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Line 5: | Line 5: | ||
100uM AmpFV6.2Sol 0.4ul | 100uM AmpFV6.2Sol 0.4ul | ||
100uM AmpRV6.2Sol 0.4ul | 100uM AmpRV6.2Sol 0.4ul | ||
50X SYBG I 0. | 50X SYBG I 0.4ul | ||
JumpStart Taq 1ul | |||
H2O 79ul | H2O 79ul | ||
94C 3min -> (94C 45sec -> 56C 1min -> 72C 1min) x 8 -> (94C 30sec -> 72C 1min) x 10 -> 72C 3min -> 4C hold. | 94C 3min -> (94C 45sec -> 56C 1min -> 72C 1min) x 8 -> (94C 30sec -> 72C 1min) x 10 -> 72C 3min -> 4C hold. | ||
[[Image:2007-12-24-E55kv1-exon-capture-PCR.png|300px]] | |||
PAGE purification. | |||
[[Image:2007-12-27-E55kv1-PCR-PAGE.png|300px]] |
Latest revision as of 02:34, 28 December 2007
Exp.1 PCR on the capturing reaction started on 12/21[edit]
Template: 10ul 10X JumpStart buffer: 9ul 25mM dNTP 1ul 100uM AmpFV6.2Sol 0.4ul 100uM AmpRV6.2Sol 0.4ul 50X SYBG I 0.4ul JumpStart Taq 1ul H2O 79ul
94C 3min -> (94C 45sec -> 56C 1min -> 72C 1min) x 8 -> (94C 30sec -> 72C 1min) x 10 -> 72C 3min -> 4C hold.
File:2007-12-24-E55kv1-exon-capture-PCR.png
PAGE purification.
File:2007-12-27-E55kv1-PCR-PAGE.png