Kun:LabNotes/CpgSeq/2007-12-30: Difference between revisions

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Line 8: Line 8:
     50x SYBG I:    0.2ul
     50x SYBG I:    0.2ul
   
   
94C 3min -> (94C 30sec -> 72C 30sec) x 20 -> 72C 3min -> 4C hold.
94C 3min -> (94C 30sec -> 72C 30sec) x 18 -> 72C 3min -> 4C hold.
     Real-time Curves
     Real-time Curves
     [[Image:2007-12-30-CpG-PAGE-PCR.png|400px]]
     [[Image:2007-12-30-CpG-PAGE-PCR.png|400px]]

Revision as of 23:37, 30 December 2007

Exp.1 Perform PCR on the gel size selected fragment from the 12-23-07 PCR products

   Into 100ul of eluted DNA, add:
   25mM dNTP:     0.6ul
   100uM AmpSolF: 0.2ul
   100uM AmpSolR: 0.2ul
   JumpStart Taq: 0.5ul
   50x SYBG I:    0.2ul

94C 3min -> (94C 30sec -> 72C 30sec) x 18 -> 72C 3min -> 4C hold.

   Real-time Curves
   File:2007-12-30-CpG-PAGE-PCR.png